Restriction map for
pFN21AE1944
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BcgI BciVI BclI BfaI BfrI
BlnI BsaBI BsiWI BsmI BspDI
BspLU11I BspMI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
EarI Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Alw44I 1311 GTGCAC G^TGCAC
ApaLI 1311 GTGCAC G^TGCAC
AspHI 1311 GTGCAC G[AT]GC[AT]^C
BalI 232 TGGCCA TGG^CCA
BamHI 375 GGATCC G^GATCC
BbeI 314 GGCGCC GGCGC^C
BbrPI 718 CACGTG CAC^GTG
BbsI 601 GAAGAC GAAGAC(2/6)
BglI 952 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1469 AGATCT A^GATCT
BlpI 729 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 729 GCTGAGC GC^T[ACGT]AGC
BpuAI 601 GAAGAC GAAGAC(2/6)
BsaAI 718 CACGTG [CT]AC^GT[GA]
BsaI 1342 GGTCTC GGTCTC(1/5)
BsiEI 397 CGGCCG CG[GA][CT]^CG
BsiHKAI 1311 GTGCAC G[AT]GC[AT]^C
BsmBI 1411 GAGACG CGTCTC(1/5)
BsmFI 573 GGGAC GGGAC(10/14)
Bsp1407I 903 TGTACA T^GTACA
BspHI 804 TCATGA T^CATGA
BsrDI 1125 CATTGC GCAATG(2/0)
BsrGI 903 TGTACA T^GTACA
BssHII 261 GCGCGC G^CGCGC
CelII 729 GCTGAGC GC^T[ACGT]AGC
DraIII 1362 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 778 GACACGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 397 CGGCCG C^GGCCG
EclXI 397 CGGCCG C^GGCCG
Eco52I 397 CGGCCG C^GGCCG
FseI 398 GGCCGGCC GGCCGG^CC
KasI 314 GGCGCC G^GCGCC
MaeII 719 ACGT A^CGT
MluNI 232 TGGCCA TGG^CCA
MscI 232 TGGCCA TGG^CCA
NarI 314 GGCGCC GG^CGCC
PflMI 1011 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 718 CACGTG CAC^GTG
PmlI 718 CACGTG CAC^GTG
RcaI 804 TCATGA T^CATGA
SfcI 964 CTACAG C^T[GA][CT]AG
SfoI 314 GGCGCC GGC^GCC
SphI 1298 GCATGC GCATG^C
SspBI 903 TGTACA T^GTACA
Tsp45I 980 GTGAC ^GT[GC]AC
Tsp509I 1160 AATT ^AATT
Van91I 1011 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 459 CCAGTACGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 314 GGCGCC G[GA]^CG[CT]C
1413 GACGCC
AlwI 376 GATCC GGATC(4/5)
375 GGATC
Aor51HI 237 AGCGCT AGC^GCT
1218 AGCGCT
BbvCI 736 CCTCAGC CCTCAGC(-5/-2)
685 GCTGAGG
BsaHI 314 GGCGCC G[GA]^CG[CT]C
1413 GACGCC
BstYI 375 GGATCC [GA]^GATC[CT]
1469 AGATCT
DpnII 376 GATC ^GATC
1470 GATC
DsaI 304 CCGCGG C^C[GA][CT]GG
410 CCGCGG
Eco47III 237 AGCGCT AGC^GCT
1218 AGCGCT
EcoNI 1266 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1425 CCTGACTGAGG
HgaI 604 GACGC GACGC(5/10)
1413 GACGC
Hin1I 314 GGCGCC G[GA]^CG[CT]C
1413 GACGCC
HphI 979 GGTGA GGTGA(8/7)
650 TCACC
KspI 304 CCGCGG CCGC^GG
410 CCGCGG
MaeIII 214 GTAAC ^GT[ACGT]AC
980 GTGAC
MboI 376 GATC ^GATC
1470 GATC
MflI 375 GGATCC [GA]^GATC[CT]
1469 AGATCT
MslI 135 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1185 CATTGATGTG
NaeI 265 GCCGGC GCC^GGC
399 GCCGGC
NdeII 376 GATC ^GATC
1470 GATC
NgoMIV 265 GCCGGC G^CCGGC
399 GCCGGC
NspI 416 GCATGT [GA]CATG^[CT]
1298 GCATGC
SacII 304 CCGCGG CCGC^GG
410 CCGCGG
Sau3AI 376 GATC ^GATC
1470 GATC
SfaNI 1291 GATGC GCATC(5/9)
1352 GCATC
XhoII 375 GGATCC [GA]^GATC[CT]
1469 AGATCT
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwNI ApaI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsaWI BseAI BseRI BsgI
BsiYI BslI BsmAI BsoBI Bsp1286I
BspEI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboII MnlI
MroI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau96I ScrFI
SmaI TfiI TseI TspRI XmaCI
XmaI