Restriction map for
pFN21AE3176
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AvrII BbeI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI FbaI
FseI HaeII Hin1I HindIII HpaI
KasI KspI MaeI MaeII MamI
MfeI MluI MseI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 199 GGTACC G^GTACC
AccIII 881 TCCGGA T^CCGGA
Alw44I 211 GTGCAC G^TGCAC
ApaI 920 GGGCCC GGGCC^C
ApaLI 211 GTGCAC G^TGCAC
Asp718I 199 GGTACC G^GTACC
AvaI 134 CCCGGG C^[CT]CG[GA]G
BamHI 1202 GGATCC G^GATCC
BanI 199 GGTACC G^G[CT][GA]CC
BbsI 475 GAAGAC GAAGAC(2/6)
BbvCI 728 CCTCAGC CCTCAGC(-5/-2)
BcgI 119 GCAAGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 385 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 475 GAAGAC GAAGAC(2/6)
BsaWI 881 TCCGGA [AT]^CCGG[AT]
BseAI 881 TCCGGA T^CCGGA
BsmBI 129 CGTCTC CGTCTC(1/5)
BsoBI 134 CCCGGG C^[CT]CG[GA]G
BspEI 881 TCCGGA T^CCGGA
BspMI 762 GCAGGT ACCTGC(4/8)
BsrFI 340 ACCGGC [GA]^CCGG[CT]
BssSI 1036 CACGAG CACGAG(-5/-1)
BstAPI 353 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 340 ACCGGC [GA]^CCGG[CT]
EcoNI 63 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1213 GATATC GAT^ATC
EcoT22I 677 ATGCAT ATGCA^T
HgaI 324 GACGC GACGC(5/10)
HincII 1087 GTTGAC GT[CT]^[GA]AC
HindII 1087 GTTGAC GT[CT]^[GA]AC
KpnI 199 GGTACC GGTAC^C
MroI 881 TCCGGA T^CCGGA
NdeI 315 CATATG CA^TATG
NsiI 677 ATGCAT ATGCA^T
PflMI 1162 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 298 GAGTC GAGTC(4/5)
PpuMI 655 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1207 CTGCAG CTGCA^G
SacI 889 GAGCTC GAGCT^C
SbfI 1206 CCTGCAGG CCTGCA^GG
SfcI 1207 CTGCAG C^T[GA][CT]AG
SmaI 134 CCCGGG CCC^GGG
Sse8387I 1206 CCTGCAGG CCTGCA^GG
TfiI 154 GAATC G^A[AT]TC
Van91I 1162 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 354 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
1163 CAGACCCTG
Asp700I 174 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
504 GAAGAGCTTC
AviII 717 TGCGCA TGC^GCA
746 TGCGCA
BalI 167 TGGCCA TGG^CCA
311 TGGCCA
BsaI 288 GGTCTC GGTCTC(1/5)
1075 GGTCTC
BseRI 925 CTCCTC GAGGAG(10/8)
685 GAGGAG
BsmFI 29 GGGAC GGGAC(10/14)
655 GGGAC
BsrDI 347 GCAATG GCAATG(2/0)
528 GCAATG
BstYI 642 GGATCT [GA]^GATC[CT]
1202 GGATCC
DpnII 643 GATC ^GATC
1203 GATC
DsaI 517 CCGTGG C^C[GA][CT]GG
1056 CCACGG
EarI 425 CTCTTC CTCTTC(1/4)
504 GAAGAG
FspI 717 TGCGCA TGC^GCA
746 TGCGCA
HinfI 154 GAATC G^A[ACGT]TC
298 GAGTC
Ksp632I 425 CTCTTC CTCTTC(1/4)
504 GAAGAG
MaeIII 205 GTCAC ^GT[ACGT]AC
615 GTCAC
MboI 643 GATC ^GATC
1203 GATC
MflI 642 GGATCT [GA]^GATC[CT]
1202 GGATCC
MluNI 167 TGGCCA TGG^CCA
311 TGGCCA
MscI 167 TGGCCA TGG^CCA
311 TGGCCA
MspA1I 49 CAGCTG C[AC]G^C[GT]G
388 CAGCTG
NdeII 643 GATC ^GATC
1203 GATC
NspI 381 GCATGC [GA]CATG^[CT]
758 GCATGC
PvuII 49 CAGCTG CAG^CTG
388 CAGCTG
SapI 504 GAAGAGC GCTCTTC(1/4)
424 GCTCTTC
Sau3AI 643 GATC ^GATC
1203 GATC
ScaI 785 AGTACT AGT^ACT
806 AGTACT
SexAI 68 ACCAGGT A^CC[AT]GGT
491 ACCAGGT
SphI 381 GCATGC GCATG^C
758 GCATGC
Tsp45I 205 GTCAC ^GT[GC]AC
615 GTCAC
XhoII 642 GGATCT [GA]^GATC[CT]
1202 GGATCC
XmnI 174 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
504 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI StyI
TseI TspRI