Restriction map for
pFN21AE1694
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbsI
BcgI BciVI BfrI BglI BglII
BlnI BpmI BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MseI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I TspRI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 225 CCGC CCGC(-3/-1)
AcyI 304 GACGCC G[GA]^CG[CT]C
AflIII 317 ACGTGT A^C[GA][CT]GT
BalI 143 TGGCCA TGG^CCA
BanII 567 GAGCTC G[GA]GC[CT]^C
BbrPI 159 CACGTG CAC^GTG
BbvCI 394 CCTCAGC CCTCAGC(-5/-2)
BclI 26 TGATCA T^GATCA
BcnI 285 CCGGG CC^[GC]GG
BfaI 400 CTAG C^TAG
BsaHI 304 GACGCC G[GA]^CG[CT]C
BseRI 418 CTCCTC GAGGAG(10/8)
BsmAI 138 GAGAC GTCTC(1/5)
BsrFI 528 GCCGGC [GA]^CCGG[CT]
CfoI 544 GCGC GCG^C
Cfr10I 528 GCCGGC [GA]^CCGG[CT]
DraIII 557 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 464 CCGTGG C^C[GA][CT]GG
EcoNI 394 CCTCAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 26 TGATCA T^GATCA
HaeII 543 AGCGCC [GA]GCGC^[CT]
HgaI 304 GACGC GACGC(5/10)
HhaI 544 GCGC GCG^C
Hin1I 304 GACGCC G[GA]^CG[CT]C
HinP1I 544 GCGC G^CGC
HphI 195 GGTGA GGTGA(8/7)
MaeI 400 CTAG C^TAG
MaeIII 157 GTCAC ^GT[ACGT]AC
MboII 244 TCTTC GAAGA(8/7)
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
MspA1I 523 CAGCTG C[AC]G^C[GT]G
MwoI 232 GCCCCACTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 528 GCCGGC GCC^GGC
NciI 285 CCGGG CC^[GC]GG
NgoMIV 528 GCCGGC G^CCGGC
PflMI 556 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 366 GACTC GAGTC(4/5)
PmaCI 159 CACGTG CAC^GTG
PmlI 159 CACGTG CAC^GTG
PvuII 523 CAGCTG CAG^CTG
SacI 567 GAGCTC GAGCT^C
Tsp45I 157 GTCAC ^GT[GC]AC
Van91I 556 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 83 GTAC GT^AC
410 GTAC
AspHI 50 GTGCTC G[AT]GC[AT]^C
567 GAGCTC
BamHI 43 GGATCC G^GATCC
94 GGATCC
BlpI 271 GCTGAGC GC^T[ACGT]AGC
569 GCTCAGC
BmyI 50 GTGCTC G[AGT]GC[ACT]^C
567 GAGCTC
Bpu1102I 271 GCTGAGC GC^T[ACGT]AGC
569 GCTCAGC
BsaAI 159 CACGTG [CT]AC^GT[GA]
316 TACGTG
BsgI 277 CTGCAC GTGCAG(16/14)
321 GTGCAG
BsiHKAI 50 GTGCTC G[AT]GC[AT]^C
567 GAGCTC
Bsp1286I 50 GTGCTC G[AGT]GC[ACT]^C
567 GAGCTC
BsrI 141 ACTGG ACTGG(1/-1)
589 CCAGT
BstYI 43 GGATCC [GA]^GATC[CT]
94 GGATCC
CelII 271 GCTGAGC GC^T[ACGT]AGC
569 GCTCAGC
EaeI 143 TGGCCA [CT]^GGCC[GA]
461 TGGCCG
EcoT14I 132 CCAAGG C^C[AT][AT]GG
342 CCTTGG
HapII 285 CCGG C^CGG
529 CCGG
HpaII 285 CCGG C^CGG
529 CCGG
MflI 43 GGATCC [GA]^GATC[CT]
94 GGATCC
MslI 312 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
554 CACCACCATG
MspI 285 CCGG C^CGG
529 CCGG
RsaI 83 GTAC GT^AC
410 GTAC
SfaNI 9 GATGC GCATC(5/9)
311 GCATC
StyI 132 CCAAGG C^C[AT][AT]GG
342 CCTTGG
TfiI 78 GATTC G^A[AT]TC
434 GATTC
XhoII 43 GGATCC [GA]^GATC[CT]
94 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeII MboI MnlI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI