Restriction map for
pFN21AE5410
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 766 GTCTAC GT^[AC][GT]AC
AfaI 171 GTAC GT^AC
AlwI 582 GATCC GGATC(4/5)
AocI 867 CCTGAGG CC^T[ACGT]AGG
ApaI 252 GGGCCC GGGCC^C
AvaI 54 CCCGGG C^[CT]CG[GA]G
BbvCI 669 GCTGAGG CCTCAGC(-5/-2)
BbvI 420 GCAGC GCAGC(8/12)
BglII 293 AGATCT A^GATCT
BpmI 772 CTCCAG CTGGAG(16/14)
BsaAI 361 TACGTG [CT]AC^GT[GA]
BsaBI 294 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 817 GGTCTC GGTCTC(1/5)
BseRI 717 CTCCTC GAGGAG(10/8)
BsgI 484 CTGCAC GTGCAG(16/14)
BsiEI 504 CGATCG CG[GA][CT]^CG
BsmI 892 GCATTC GAATGC(1/-1)
BsoBI 54 CCCGGG C^[CT]CG[GA]G
BsrDI 759 CATTGC GCAATG(2/0)
BsrI 599 CCAGT ACTGG(1/-1)
BssSI 216 CACGAG CACGAG(-5/-1)
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
CfoI 142 GCGC GCG^C
DrdI 280 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
FauI 407 CCCGC CCCGC(4/6)
Fnu4HI 420 GCAGC GC^[ACGT]GC
HhaI 142 GCGC GCG^C
HinP1I 142 GCGC G^CGC
HindIII 701 AAGCTT A^AGCTT
HpaI 4 GTTAAC GTT^AAC
ItaI 420 GCAGC GC^[ACGT]GC
MaeII 362 ACGT A^CGT
MamI 294 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 421 CAGCTG C[AC]G^C[GT]G
PvuI 504 CGATCG CGAT^CG
PvuII 421 CAGCTG CAG^CTG
RsaI 171 GTAC GT^AC
SfcI 747 CTACAG C^T[GA][CT]AG
SmaI 54 CCCGGG CCC^GGG
TaqI 549 TCGA T^CGA
TseI 420 GCAGC G^C[AT]GC
Tsp45I 736 GTCAC ^GT[GC]AC
Tsp509I 546 AATT ^AATT
TthHB8I 549 TCGA T^CGA
XcmI 266 CCATCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 54 CCCGGG C^CCGGG
XmaI 54 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 408 CCGC CCGC(-3/-1)
693 GCGG
BanII 252 GGGCCC G[GA]GC[CT]^C
922 GGGCTC
BfaI 109 CTAG C^TAG
451 CTAG
BsmAI 640 GTCTC GTCTC(1/5)
818 GTCTC
BstEII 735 GGTCACC G^GT[ACGT]ACC
882 GGTAACC
BstPI 735 GGTCACC G^GT[ACGT]ACC
882 GGTAACC
BstYI 293 AGATCT [GA]^GATC[CT]
581 AGATCC
EcoO65I 735 GGTCACC G^GT[ACGT]ACC
882 GGTAACC
HaeIII 253 GGCC GG^CC
435 GGCC
HapII 22 CCGG C^CGG
55 CCGG
HincII 4 GTTAAC GT[CT]^[GA]AC
241 GTCAAC
HindII 4 GTTAAC GT[CT]^[GA]AC
241 GTCAAC
HpaII 22 CCGG C^CGG
55 CCGG
HphI 212 TCACC GGTGA(8/7)
737 TCACC
MaeI 109 CTAG C^TAG
451 CTAG
MaeIII 736 GTCAC ^GT[ACGT]AC
883 GTAAC
MflI 293 AGATCT [GA]^GATC[CT]
581 AGATCC
MspI 22 CCGG C^CGG
55 CCGG
MwoI 135 GCTGTCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
427 GCATCTGTGGC
PflMI 231 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
653 CCATTACCTGG
PpuMI 533 AGGTCCC [GA]G^G[AT]CC[CT]
709 GGGACCT
SfaNI 329 GCATC GCATC(5/9)
427 GCATC
Van91I 231 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
653 CCATTACCTGG
XhoII 293 AGATCT [GA]^GATC[CT]
581 AGATCC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AvaII BbsI BcnI BmyI
BpuAI BsaJI BsiYI BslI BsmFI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FokI HinfI
Ksp632I MboI MboII MnlI MseI
MvaI NciI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI Tru9I TspRI