Restriction map for
pFN21AE5975
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BalI
BbrPI BbsI BbvCI BcgI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FseI HgaI HincII HindII
HindIII HinfI HpaI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI MvnI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 419 GTCTAC GT^[AC][GT]AC
AccIII 674 TCCGGA T^CCGGA
AfaI 174 GTAC GT^AC
AocI 781 CCTAAGG CC^T[ACGT]AGG
AspHI 287 GTGCTC G[AT]GC[AT]^C
AvaII 228 GGTCC G^G[AT]CC
AviII 185 TGCGCA TGC^GCA
BamHI 677 GGATCC G^GATCC
BanII 642 GGGCTC G[GA]GC[CT]^C
BciVI 656 GTATCC GTATCC(6/5)
BclI 731 TGATCA T^GATCA
BcnI 411 CCGGG CC^[GC]GG
BfaI 427 CTAG C^TAG
BglI 623 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 402 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC
BsaWI 674 TCCGGA [AT]^CCGG[AT]
BseAI 674 TCCGGA T^CCGGA
BsiHKAI 287 GTGCTC G[AT]GC[AT]^C
BspEI 674 TCCGGA T^CCGGA
BsrFI 260 GCCGGC [GA]^CCGG[CT]
BstEII 837 GGTCACC G^GT[ACGT]ACC
BstPI 837 GGTCACC G^GT[ACGT]ACC
Bsu36I 781 CCTAAGG CC^T[ACGT]AGG
CelII 402 GCTGAGC GC^T[ACGT]AGC
Cfr10I 260 GCCGGC [GA]^CCGG[CT]
Eco81I 781 CCTAAGG CC^T[ACGT]AGG
EcoO65I 837 GGTCACC G^GT[ACGT]ACC
FbaI 731 TGATCA T^GATCA
FspI 185 TGCGCA TGC^GCA
MaeI 427 CTAG C^TAG
MaeII 217 ACGT A^CGT
MroI 674 TCCGGA T^CCGGA
MseI 578 TTAA T^TAA
MspA1I 553 CAGCTG C[AC]G^C[GT]G
NaeI 260 GCCGGC GCC^GGC
NciI 411 CCGGG CC^[GC]GG
NgoMIV 260 GCCGGC G^CCGGC
NheI 426 GCTAGC G^CTAGC
PvuII 553 CAGCTG CAG^CTG
RsaI 174 GTAC GT^AC
SapI 75 GCTCTTC GCTCTTC(1/4)
SfaNI 151 GATGC GCATC(5/9)
StuI 704 AGGCCT AGG^CCT
Tru9I 578 TTAA T^TAA
Tsp509I 121 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 263 GGCGCC G[GA]^CG[CT]C
319 GGCGCC
BbeI 263 GGCGCC GGCGC^C
319 GGCGCC
BmyI 287 GTGCTC G[AGT]GC[ACT]^C
642 GGGCTC
BsaHI 263 GGCGCC G[GA]^CG[CT]C
319 GGCGCC
BsaI 141 GGTCTC GGTCTC(1/5)
738 GGTCTC
BseRI 610 CTCCTC GAGGAG(10/8)
720 CTCCTC
Bsp1286I 287 GTGCTC G[AGT]GC[ACT]^C
642 GGGCTC
BspMI 101 ACCTGC ACCTGC(4/8)
715 ACCTGC
BsrI 256 ACTGG ACTGG(1/-1)
162 CCAGT
BstYI 536 AGATCC [GA]^GATC[CT]
677 GGATCC
DraIII 324 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
409 CACCGGGTG
FauI 519 CCCGC CCCGC(4/6)
691 GCGGG
HaeII 263 GGCGCC [GA]GCGC^[CT]
319 GGCGCC
Hin1I 263 GGCGCC G[GA]^CG[CT]C
319 GGCGCC
KasI 263 GGCGCC G^GCGCC
319 GGCGCC
MflI 536 AGATCC [GA]^GATC[CT]
677 GGATCC
MslI 12 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
129 CATTGTGGTG
NarI 263 GGCGCC GG^CGCC
319 GGCGCC
NcoI 17 CCATGG C^CATGG
347 CCATGG
PstI 550 CTGCAG CTGCA^G
586 CTGCAG
SfoI 263 GGCGCC GGC^GCC
319 GGCGCC
XcmI 246 CCACCTCCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
803 CCACTGACCCTCTGG
XhoII 536 AGATCC [GA]^GATC[CT]
677 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI BanI BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfcI StyI TseI
Tsp45I TspRI