Restriction map for
pFN21AE5438
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BbeI BbrPI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII ItaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I TseI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AcsI 37 GAATTT [GA]^AATT[CT]
AfaI 423 GTAC GT^AC
ApaI 412 GGGCCC GGGCC^C
ApoI 37 GAATTT [GA]^AATT[CT]
BamHI 573 GGATCC G^GATCC
BanII 412 GGGCCC G[GA]GC[CT]^C
BbvCI 498 GCTGAGG CCTCAGC(-5/-2)
BmyI 412 GGGCCC G[AGT]GC[ACT]^C
BsmAI 10 GTCTC GTCTC(1/5)
BsmI 334 GAATGC GAATGC(1/-1)
Bsp1286I 412 GGGCCC G[AGT]GC[ACT]^C
BspHI 767 TCATGA T^CATGA
BstAPI 784 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 63 CATCC GGATG(2/0)
BstXI 659 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 573 GGATCC [GA]^GATC[CT]
DraII 411 GGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 853 CTCTTC CTCTTC(1/4)
EcoNI 810 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 411 GGGGCCC [GA]G^G[ACGT]CC[CT]
FokI 63 CATCC GGATG(9/13)
Ksp632I 853 CTCTTC CTCTTC(1/4)
MflI 573 GGATCC [GA]^GATC[CT]
NdeI 359 CATATG CA^TATG
NspI 721 ACATGC [GA]CATG^[CT]
RcaI 767 TCATGA T^CATGA
RsaI 423 GTAC GT^AC
SfcI 740 CTGTAG C^T[GA][CT]AG
XhoII 573 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 230 CCGC CCGC(-3/-1)
366 CCGC
AlwI 574 GATCC GGATC(4/5)
573 GGATC
BbsI 112 GTCTTC GAAGAC(2/6)
820 GTCTTC
BfaI 322 CTAG C^TAG
736 CTAG
BpmI 298 CTCCAG CTGGAG(16/14)
250 CTGGAG
BpuAI 112 GTCTTC GAAGAC(2/6)
820 GTCTTC
BseRI 726 CTCCTC GAGGAG(10/8)
808 CTCCTC
BstEII 489 GGTGACC G^GT[ACGT]ACC
843 GGTCACC
BstPI 489 GGTGACC G^GT[ACGT]ACC
843 GGTCACC
EcoO65I 489 GGTGACC G^GT[ACGT]ACC
843 GGTCACC
EcoT14I 48 CCTTGG C^C[AT][AT]GG
815 CCAAGG
MaeI 322 CTAG C^TAG
736 CTAG
MspA1I 329 CAGCTG C[AC]G^C[GT]G
496 CAGCTG
PvuII 329 CAGCTG CAG^CTG
496 CAGCTG
SfaNI 237 GATGC GCATC(5/9)
894 GCATC
StyI 48 CCTTGG C^C[AT][AT]GG
815 CCAAGG
XcmI 416 CCAGACGGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
659 CCAGAATTGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EcoRII HaeIII HinfI HphI
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TfiI Tsp45I Tsp509I
TspRI