Restriction map for
pFN21AE9603
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstF5I BstPI BstUI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FokI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AfaI 305 GTAC GT^AC
AhdI 418 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 418 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 540 GAGCCC G[GA]GC[CT]^C
BcgI 553 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 140 TGATCA T^GATCA
BfaI 40 CTAG C^TAG
BmyI 540 GAGCCC G[AGT]GC[ACT]^C
BsaWI 316 TCCGGT [AT]^CCGG[AT]
BsiYI 543 CCCAGTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 543 CCCAGTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 540 GAGCCC G[AGT]GC[ACT]^C
BspHI 585 TCATGA T^CATGA
BsrBI 495 GAGCGG CCGCTC(-3/-3)
BsrFI 84 GCCGGC [GA]^CCGG[CT]
BstAPI 19 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 561 TTCGAA TT^CGAA
BstXI 395 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 193 GCGC GCG^C
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
Cfr13I 150 GGCCC G^G[ACGT]CC
DpnII 141 GATC ^GATC
DraII 149 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 209 TGGCCG [CT]^GGCC[GA]
Eam1105I 418 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 537 GAAGAG CTCTTC(1/4)
EcoO109I 149 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 518 CCAAGG C^C[AT][AT]GG
FauI 497 GCGGG CCCGC(4/6)
FbaI 140 TGATCA T^GATCA
HhaI 193 GCGC GCG^C
HinP1I 193 GCGC G^CGC
HindIII 328 AAGCTT A^AGCTT
Ksp632I 537 GAAGAG CTCTTC(1/4)
MaeI 40 CTAG C^TAG
MaeII 227 ACGT A^CGT
MboI 141 GATC ^GATC
MspA1I 212 CCGCTG C[AC]G^C[GT]G
NaeI 84 GCCGGC GCC^GGC
NdeII 141 GATC ^GATC
NgoMIV 84 GCCGGC G^CCGGC
NlaIII 586 CATG CATG^
NspV 561 TTCGAA TT^CGAA
PleI 418 GACTC GAGTC(4/5)
PstI 17 CTGCAG CTGCA^G
RcaI 585 TCATGA T^CATGA
RsaI 305 GTAC GT^AC
SapI 537 GAAGAGC GCTCTTC(1/4)
Sau3AI 141 GATC ^GATC
Sau96I 150 GGCCC G^G[ACGT]CC
SfaNI 248 GCATC GCATC(5/9)
SfcI 17 CTGCAG C^T[GA][CT]AG
SfuI 561 TTCGAA TT^CGAA
StyI 518 CCAAGG C^C[AT][AT]GG
TaqI 562 TCGA T^CGA
Tsp509I 572 AATT ^AATT
TthHB8I 562 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwNI 20 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
380 CAGTTTCTG
BbvI 19 GCAGC GCAGC(8/12)
214 GCTGC
BsmFI 416 GGGAC GGGAC(10/14)
258 GTCCC
HapII 85 CCGG C^CGG
317 CCGG
HpaII 85 CCGG C^CGG
317 CCGG
MaeIII 162 GTCAC ^GT[ACGT]AC
426 GTCAC
MboII 234 GAAGA GAAGA(8/7)
537 GAAGA
MseI 326 TTAA T^TAA
593 TTAA
MspI 85 CCGG C^CGG
317 CCGG
NlaIV 319 GGTTCC GG[ACGT]^[ACGT]CC
514 GGAGCC
SmlI 196 CTCAAG C^T[CT][GA]AG
262 CTCAAG
TfiI 284 GAATC G^A[AT]TC
345 GATTC
Tru9I 326 TTAA T^TAA
593 TTAA
TseI 19 GCAGC G^C[AT]GC
214 GCTGC
Tsp45I 162 GTCAC ^GT[GC]AC
426 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BsaJI BsrI BssKI
BstNI Cac8I DdeI EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MnlI
MvaI MwoI ScrFI TspRI