Restriction map for
pFN21AE5232
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HindIII
HpaI HphI KasI KpnI KspI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SapI SbfI
ScaI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI SwaI TfiI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 571 GTCGAC GT^[AC][GT]AC
AcyI 463 GACGCC G[GA]^CG[CT]C
AluI 668 AGCT AG^CT
AvaI 325 CCCGAG C^[CT]CG[GA]G
BanI 723 GGCACC G^G[CT][GA]CC
BbvCI 649 CCTCAGC CCTCAGC(-5/-2)
BfaI 620 CTAG C^TAG
BsaHI 463 GACGCC G[GA]^CG[CT]C
BsiEI 566 CGGCCG CG[GA][CT]^CG
BsoBI 325 CCCGAG C^[CT]CG[GA]G
Bsp1407I 23 TGTACA T^GTACA
BsrBI 30 GAGCGG CCGCTC(-3/-3)
BsrFI 75 GCCGGC [GA]^CCGG[CT]
BsrGI 23 TGTACA T^GTACA
BsrI 71 ACTGG ACTGG(1/-1)
BstXI 360 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
DpnII 739 GATC ^GATC
DsaI 257 CCACGG C^C[GA][CT]GG
EagI 566 CGGCCG C^GGCCG
EarI 435 GAAGAG CTCTTC(1/4)
EclXI 566 CGGCCG C^GGCCG
Eco52I 566 CGGCCG C^GGCCG
HaeII 613 AGCGCC [GA]GCGC^[CT]
HgaI 463 GACGC GACGC(5/10)
Hin1I 463 GACGCC G[GA]^CG[CT]C
HincII 571 GTCGAC GT[CT]^[GA]AC
HindII 571 GTCGAC GT[CT]^[GA]AC
Ksp632I 435 GAAGAG CTCTTC(1/4)
MaeI 620 CTAG C^TAG
MboI 739 GATC ^GATC
MseI 55 TTAA T^TAA
NaeI 75 GCCGGC GCC^GGC
NdeII 739 GATC ^GATC
NgoMIV 75 GCCGGC G^CCGGC
NruI 741 TCGCGA TCG^CGA
PshAI 564 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 667 GAGCTC GAGCT^C
SalI 571 GTCGAC G^TCGAC
Sau3AI 739 GATC ^GATC
SspBI 23 TGTACA T^GTACA
Tru9I 55 TTAA T^TAA
Tsp509I 383 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 24 GTAC GT^AC
174 GTAC
AspHI 88 GAGCAC G[AT]GC[AT]^C
667 GAGCTC
BanII 322 GAGCCC G[GA]GC[CT]^C
667 GAGCTC
BsiHKAI 88 GAGCAC G[AT]GC[AT]^C
667 GAGCTC
BsmAI 139 GAGAC GTCTC(1/5)
305 GAGAC
BsmBI 139 GAGACG CGTCTC(1/5)
305 GAGACG
BssSI 136 CACGAG CACGAG(-5/-1)
592 CTCGTG
EcoT14I 313 CCTTGG C^C[AT][AT]GG
479 CCAAGG
HinfI 153 GAGTC G^A[ACGT]TC
582 GAGTC
MslI 15 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
361 CACGCTGGTG
MspA1I 300 CAGCGG C[AC]G^C[GT]G
430 CCGCTG
NspI 14 GCATGC [GA]CATG^[CT]
714 GCATGC
PleI 153 GAGTC GAGTC(4/5)
582 GAGTC
RsaI 24 GTAC GT^AC
174 GTAC
SexAI 38 ACCTGGT A^CC[AT]GGT
113 ACCAGGT
SphI 14 GCATGC GCATG^C
714 GCATGC
StuI 266 AGGCCT AGG^CCT
698 AGGCCT
StyI 313 CCTTGG C^C[AT][AT]GG
479 CCAAGG
TaqI 245 TCGA T^CGA
572 TCGA
TspRI 70 CACTG CA[GC]TG[ACGT][ACGT]^
411 CACTG
TthHB8I 245 TCGA T^CGA
572 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AvaII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MnlI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI TseI