Restriction map for
pFN21AB7066
##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BsmAI BsmBI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 1983 TCCGGA T^CCGGA
AflIII 1559 ACACGT A^C[GA][CT]GT
AgeI 1268 ACCGGT A^CCGGT
AspHI 1903 GAGCAC G[AT]GC[AT]^C
BamHI 2103 GGATCC G^GATCC
BanI 2325 GGCGCC G^G[CT][GA]CC
BbeI 2325 GGCGCC GGCGC^C
BbrPI 1078 CACGTG CAC^GTG
BciVI 1941 GTATCC GTATCC(6/5)
BlpI 583 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 583 GCTGAGC GC^T[ACGT]AGC
BsaAI 1078 CACGTG [CT]AC^GT[GA]
BseAI 1983 TCCGGA T^CCGGA
BsiHKAI 1903 GAGCAC G[AT]GC[AT]^C
BsmI 28 GAATGC GAATGC(1/-1)
BspEI 1983 TCCGGA T^CCGGA
BspHI 1721 TCATGA T^CATGA
BstAPI 23 GCAACGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1614 GGTCACC G^GT[ACGT]ACC
BstPI 1614 GGTCACC G^GT[ACGT]ACC
CelII 583 GCTGAGC GC^T[ACGT]AGC
DrdI 336 GACCCCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1029 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1614 GGTCACC G^GT[ACGT]ACC
KasI 2325 GGCGCC G^GCGCC
KspI 2297 CCGCGG CCGC^GG
MroI 1983 TCCGGA T^CCGGA
NarI 2325 GGCGCC GG^CGCC
NcoI 1637 CCATGG C^CATGG
PinAI 1268 ACCGGT A^CCGGT
PmaCI 1078 CACGTG CAC^GTG
PmlI 1078 CACGTG CAC^GTG
RcaI 1721 TCATGA T^CATGA
SacII 2297 CCGCGG CCGC^GG
SapI 987 GAAGAGC GCTCTTC(1/4)
SexAI 1442 ACCTGGT A^CC[AT]GGT
SfoI 2325 GGCGCC GGC^GCC
SmlI 2059 CTCAAG C^T[CT][GA]AG
TaqI 1829 TCGA T^CGA
TfiI 2186 GAATC G^A[AT]TC
Tsp509I 2394 AATT ^AATT
TthHB8I 1829 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 567 GACGCC G[GA]^CG[CT]C
2325 GGCGCC
AlwNI 1438 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1462 CAGCCCCTG
AocI 1603 CCTGAGG CC^T[ACGT]AGG
2398 CCTCAGG
Aor51HI 271 AGCGCT AGC^GCT
509 AGCGCT
ApaI 850 GGGCCC GGGCC^C
1004 GGGCCC
BbvCI 678 CCTCAGC CCTCAGC(-5/-2)
1013 CCTCAGC
BcgI 187 GCAGGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
472 GCAGTGGCCTCG
BsaHI 567 GACGCC G[GA]^CG[CT]C
2325 GGCGCC
BsaWI 1268 ACCGGT [AT]^CCGG[AT]
1983 TCCGGA
BsiWI 314 CGTACG C^GTACG
1064 CGTACG
BspMI 365 ACCTGC ACCTGC(4/8)
1658 ACCTGC
BsrBI 16 GAGCGG CCGCTC(-3/-3)
1395 GAGCGG
Bsu36I 1603 CCTGAGG CC^T[ACGT]AGG
2398 CCTCAGG
EagI 482 CGGCCG C^GGCCG
771 CGGCCG
EclXI 482 CGGCCG C^GGCCG
771 CGGCCG
Eco47III 271 AGCGCT AGC^GCT
509 AGCGCT
Eco52I 482 CGGCCG C^GGCCG
771 CGGCCG
Eco81I 1603 CCTGAGG CC^T[ACGT]AGG
2398 CCTCAGG
EcoT14I 959 CCAAGG C^C[AT][AT]GG
1637 CCATGG
Hin1I 567 GACGCC G[GA]^CG[CT]C
2325 GGCGCC
MslI 1488 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2161 CACAGGTATG
SfiI 483 GGCCGCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
948 GGCCATCCAGGCC
StuI 524 AGGCCT AGG^CCT
1211 AGGCCT
StyI 959 CCAAGG C^C[AT][AT]GG
1637 CCATGG
XcmI 1073 CCAGGCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1733 CCATCATCCAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI Tru9I TseI Tsp45I
TspRI Van91I XhoII XmaCI XmaI