Restriction map for
pFN21AE5924
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BcnI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I Cfr10I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HapII HgaI Hin1I HindIII
HpaI HpaII KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MroI MseI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp45I Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 811 AAATTT [GA]^AATT[CT]
Alw44I 652 GTGCAC G^TGCAC
AlwI 471 GATCC GGATC(4/5)
ApaLI 652 GTGCAC G^TGCAC
ApoI 811 AAATTT [GA]^AATT[CT]
Asp700I 519 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 602 CCCGAG C^[CT]CG[GA]G
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
BclI 245 TGATCA T^GATCA
BpmI 67 CTGGAG CTGGAG(16/14)
BsaI 481 GGTCTC GGTCTC(1/5)
BsgI 163 CTGCAC GTGCAG(16/14)
BsmAI 482 GTCTC GTCTC(1/5)
BsoBI 602 CCCGAG C^[CT]CG[GA]G
CfoI 696 GCGC GCG^C
DraII 721 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 714 GACTTGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 279 CCGTGG C^C[GA][CT]GG
EarI 301 CTCTTC CTCTTC(1/4)
EcoO109I 721 GGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 245 TGATCA T^GATCA
HhaI 696 GCGC GCG^C
HinP1I 696 GCGC G^CGC
HincII 684 GTCAAC GT[CT]^[GA]AC
HindII 684 GTCAAC GT[CT]^[GA]AC
Ksp632I 301 CTCTTC CTCTTC(1/4)
MaeII 884 ACGT A^CGT
PpuMI 721 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 626 CTGCAG CTGCA^G
SapI 300 GCTCTTC GCTCTTC(1/4)
ScaI 906 AGTACT AGT^ACT
SfaNI 537 GATGC GCATC(5/9)
SfcI 626 CTGCAG C^T[GA][CT]AG
TaqI 513 TCGA T^CGA
TspRI 668 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 513 TCGA T^CGA
XcmI 587 CCAAGATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 519 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 177 GTAC GT^AC
907 GTAC
AluI 239 AGCT AG^CT
299 AGCT
AlwNI 238 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
424 CAGACCCTG
AspHI 337 GTGCTC G[AT]GC[AT]^C
652 GTGCAC
BlpI 295 GCTCAGC GC^T[ACGT]AGC
755 GCTCAGC
Bpu1102I 295 GCTCAGC GC^T[ACGT]AGC
755 GCTCAGC
BsiHKAI 337 GTGCTC G[AT]GC[AT]^C
652 GTGCAC
BsrDI 657 CATTGC GCAATG(2/0)
487 GCAATG
BsrI 66 ACTGG ACTGG(1/-1)
17 CCAGT
BstF5I 75 CATCC GGATG(2/0)
783 CATCC
CelII 295 GCTCAGC GC^T[ACGT]AGC
755 GCTCAGC
DpnII 246 GATC ^GATC
471 GATC
FauI 257 GCGGG CCCGC(4/6)
451 GCGGG
FokI 75 CATCC GGATG(9/13)
783 CATCC
MboI 246 GATC ^GATC
471 GATC
MwoI 63 GCAACTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
619 GCTGTTCCTGC
NdeII 246 GATC ^GATC
471 GATC
PflMI 390 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
423 CCAGACCCTGG
PleI 925 GACTC GAGTC(4/5)
605 GAGTC
RsaI 177 GTAC GT^AC
907 GTAC
Sau3AI 246 GATC ^GATC
471 GATC
TfiI 47 GATTC G^A[AT]TC
519 GAATC
Van91I 390 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
423 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BalI BanII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cfr13I
DdeI EaeI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeI MboII
MluNI MnlI MscI MslI MvaI
NlaIII NlaIV Sau96I ScrFI TseI
Tsp509I