Restriction map for
pFN21AE6771
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI Eco47III EcoRI
EcoRV FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeII MfeI MluI
MroI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AflII 973 CTTAAG C^TTAAG
AhdI 274 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 440 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 12 CCTGAGG CC^T[ACGT]AGG
ApaI 464 GGGCCC GGGCC^C
AspEI 274 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 215 GAGCAC G[AT]GC[AT]^C
BbvCI 769 GCTGAGG CCTCAGC(-5/-2)
BclI 837 TGATCA T^GATCA
BfrI 973 CTTAAG C^TTAAG
BglI 1279 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 835 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 28 CGGCCG CG[GA][CT]^CG
BsiHKAI 215 GAGCAC G[AT]GC[AT]^C
BsmFI 74 GGGAC GGGAC(10/14)
BsmI 388 GAATGC GAATGC(1/-1)
BspMI 310 GCAGGT ACCTGC(4/8)
BssHII 60 GCGCGC G^CGCGC
BstEII 313 GGTTACC G^GT[ACGT]ACC
BstPI 313 GGTTACC G^GT[ACGT]ACC
BstYI 774 GGATCT [GA]^GATC[CT]
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
DraI 1512 TTTAAA TTT^AAA
DraII 726 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 674 CCATGG C^C[GA][CT]GG
EagI 28 CGGCCG C^GGCCG
Eam1105I 274 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 28 CGGCCG C^GGCCG
Eco52I 28 CGGCCG C^GGCCG
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
EcoNI 120 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 726 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 313 GGTTACC G^GT[ACGT]ACC
EcoT22I 390 ATGCAT ATGCA^T
FbaI 837 TGATCA T^GATCA
MamI 835 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 774 GGATCT [GA]^GATC[CT]
MspA1I 1280 CCGCTG C[AC]G^C[GT]G
NcoI 674 CCATGG C^CATGG
NsiI 390 ATGCAT ATGCA^T
PflMI 343 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 76 GACTC GAGTC(4/5)
PpuMI 726 AGGACCC [GA]G^G[AT]CC[CT]
SapI 922 GAAGAGC GCTCTTC(1/4)
SfiI 1278 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1472 CCCGGG CCC^GGG
SmlI 973 CTTAAG C^T[CT][GA]AG
SpeI 153 ACTAGT A^CTAGT
Van91I 343 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1610 CCAGACTGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 774 GGATCT [GA]^GATC[CT]
XmaCI 1472 CCCGGG C^CCGGG
XmaI 1472 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 709 GGTACC G^GTACC
1091 GGTACC
Asp718I 709 GGTACC G^GTACC
1091 GGTACC
AvaI 212 CCCGAG C^[CT]CG[GA]G
1472 CCCGGG
AvaII 727 GGACC G^G[AT]CC
1461 GGACC
BalI 1393 TGGCCA TGG^CCA
1445 TGGCCA
BbsI 1662 GAAGAC GAAGAC(2/6)
603 GTCTTC
BpmI 1491 CTCCAG CTGGAG(16/14)
809 CTGGAG
BpuAI 1662 GAAGAC GAAGAC(2/6)
603 GTCTTC
BsgI 645 CTGCAC GTGCAG(16/14)
1637 GTGCAG
BsoBI 212 CCCGAG C^[CT]CG[GA]G
1472 CCCGGG
BsrDI 796 CATTGC GCAATG(2/0)
1002 CATTGC
FauI 38 CCCGC CCCGC(4/6)
49 CCCGC
HphI 202 GGTGA GGTGA(8/7)
905 TCACC
KpnI 709 GGTACC GGTAC^C
1091 GGTACC
MluNI 1393 TGGCCA TGG^CCA
1445 TGGCCA
MscI 1393 TGGCCA TGG^CCA
1445 TGGCCA
SfaNI 409 GATGC GCATC(5/9)
500 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanI BanII BbvI BcnI BfaI
BmyI BsaI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I