Restriction map for
pFN21AE1786
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MflI
MluI MluNI MroI MscI MslI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 67 CGCG CG^CG
AcsI 637 AAATTC [GA]^AATT[CT]
AcyI 31 GGCGTC G[GA]^CG[CT]C
AfaI 801 GTAC GT^AC
ApoI 637 AAATTC [GA]^AATT[CT]
AspHI 864 GTGCTC G[AT]GC[AT]^C
BbsI 310 GAAGAC GAAGAC(2/6)
BcnI 36 CCCGG CC^[GC]GG
BmyI 864 GTGCTC G[AGT]GC[ACT]^C
BpmI 516 CTCCAG CTGGAG(16/14)
BpuAI 310 GAAGAC GAAGAC(2/6)
BsaHI 31 GGCGTC G[GA]^CG[CT]C
BsaI 521 GGTCTC GGTCTC(1/5)
BseRI 400 GAGGAG GAGGAG(10/8)
BsiHKAI 864 GTGCTC G[AT]GC[AT]^C
BsmAI 522 GTCTC GTCTC(1/5)
BsmI 462 GAATGC GAATGC(1/-1)
Bsp1286I 864 GTGCTC G[AGT]GC[ACT]^C
BspMI 219 ACCTGC ACCTGC(4/8)
BsrDI 336 CATTGC GCAATG(2/0)
BstF5I 102 GGATG GGATG(2/0)
BstUI 67 CGCG CG^CG
CfoI 66 GCGC GCG^C
DdeI 224 CTAAG C^T[ACGT]AG
DpnII 418 GATC ^GATC
DsaI 565 CCATGG C^C[GA][CT]GG
EcoT14I 565 CCATGG C^C[AT][AT]GG
FokI 102 GGATG GGATG(9/13)
HgaI 32 GCGTC GACGC(5/10)
HhaI 66 GCGC GCG^C
Hin1I 31 GGCGTC G[GA]^CG[CT]C
HinP1I 66 GCGC G^CGC
HphI 169 GGTGA GGTGA(8/7)
MaeIII 170 GTGAC ^GT[ACGT]AC
MboI 418 GATC ^GATC
MfeI 344 CAATTG C^AATTG
MspA1I 12 CAGCGG C[AC]G^C[GT]G
MunI 344 CAATTG C^AATTG
MvnI 67 CGCG CG^CG
NciI 36 CCCGG CC^[GC]GG
NcoI 565 CCATGG C^CATGG
NdeII 418 GATC ^GATC
NlaIV 615 GGGACC GG[ACGT]^[ACGT]CC
NspI 153 GCATGC [GA]CATG^[CT]
PstI 254 CTGCAG CTGCA^G
RsaI 801 GTAC GT^AC
Sau3AI 418 GATC ^GATC
SfcI 254 CTGCAG C^T[GA][CT]AG
SmlI 467 CTCAAG C^T[CT][GA]AG
SphI 153 GCATGC GCATG^C
StyI 565 CCATGG C^C[AT][AT]GG
Tsp45I 170 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AvaII 616 GGACC G^G[AT]CC
715 GGACC
BsaJI 565 CCATGG C^C[ACGT][ACGT]GG
601 CCAGGG
Cac8I 153 GCATGC GC[ACGT]^[ACGT]GC
692 GCTGGC
HapII 37 CCGG C^CGG
63 CCGG
HpaII 37 CCGG C^CGG
63 CCGG
MspI 37 CCGG C^CGG
63 CCGG
NdeI 125 CATATG CA^TATG
744 CATATG
SfaNI 766 GATGC GCATC(5/9)
772 GATGC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BsmFI BsrI BssKI BstNI Cfr13I
EcoRII Fnu4HI HaeIII HinfI ItaI
MboII MnlI MseI MvaI MwoI
NlaIII Sau96I ScrFI TfiI Tru9I
TseI Tsp509I TspRI