Restriction map for
pFN21AE3089
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 402 GTATAC GT^[AC][GT]AC
AfaI 198 GTAC GT^AC
AlwI 348 GATCC GGATC(4/5)
AvaII 41 GGTCC G^G[AT]CC
BanII 143 GAGCCC G[GA]GC[CT]^C
BfaI 532 CTAG C^TAG
BglI 295 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 143 GAGCCC G[AGT]GC[ACT]^C
BsaI 440 GGTCTC GGTCTC(1/5)
BsgI 8 CTGCAC GTGCAG(16/14)
Bsp1286I 143 GAGCCC G[AGT]GC[ACT]^C
BsrFI 38 GCCGGT [GA]^CCGG[CT]
Bst1107I 402 GTATAC GTA^TAC
BstXI 485 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 347 AGATCC [GA]^GATC[CT]
BstZ17I 402 GTATAC GTA^TAC
Cac8I 71 GCAAGC GC[ACGT]^[ACGT]GC
Cfr10I 38 GCCGGT [GA]^CCGG[CT]
MaeI 532 CTAG C^TAG
MflI 347 AGATCC [GA]^GATC[CT]
MseI 637 TTAA T^TAA
NdeI 426 CATATG CA^TATG
NlaIII 615 CATG CATG^
PflMI 309 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 538 GACTC GAGTC(4/5)
RsaI 198 GTAC GT^AC
SmlI 765 CTCAAG C^T[CT][GA]AG
TfiI 699 GAATC G^A[AT]TC
Tru9I 637 TTAA T^TAA
Van91I 309 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 425 CCATATGTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 347 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbsI 535 GAAGAC GAAGAC(2/6)
725 GAAGAC
BcnI 252 CCGGG CC^[GC]GG
333 CCCGG
BpuAI 535 GAAGAC GAAGAC(2/6)
725 GAAGAC
BsaJI 146 CCCTGG C^C[ACGT][ACGT]GG
716 CCCTGG
BspMI 525 ACCTGC ACCTGC(4/8)
579 GCAGGT
BsrBI 48 CCGCTC CCGCTC(-3/-3)
87 GAGCGG
EarI 443 CTCTTC CTCTTC(1/4)
732 GAAGAG
HaeII 29 AGCGCC [GA]GCGC^[CT]
124 AGCGCC
HinfI 538 GACTC G^A[ACGT]TC
699 GAATC
HphI 343 GGTGA GGTGA(8/7)
720 GGTGA
Ksp632I 443 CTCTTC CTCTTC(1/4)
732 GAAGAG
MslI 183 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CACAGAGATG
NciI 252 CCGGG CC^[GC]GG
333 CCCGG
PstI 82 CTGCAG CTGCA^G
382 CTGCAG
PvuII 237 CAGCTG CAG^CTG
379 CAGCTG
SfaNI 162 GATGC GCATC(5/9)
414 GATGC
SfcI 82 CTGCAG C^T[GA][CT]AG
382 CTGCAG
TaqI 55 TCGA T^CGA
456 TCGA
Tsp509I 314 AATT ^AATT
504 AATT
TspRI 385 CAGTG CA[GC]TG[ACGT][ACGT]^
603 CACTG
TthHB8I 55 TCGA T^CGA
456 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BseRI BsiYI
BslI BsmAI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI