Restriction map for
pFN21AE3112
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsiEI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tsp45I Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 14 CGCG CG^CG
AcsI 258 AAATTC [GA]^AATT[CT]
AlwI 403 GATCC GGATC(4/5)
AlwNI 337 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 258 AAATTC [GA]^AATT[CT]
AvaI 647 CTCGAG C^[CT]CG[GA]G
BanII 704 GAGCTC G[GA]GC[CT]^C
BbsI 151 GAAGAC GAAGAC(2/6)
BciVI 625 GTATCC GTATCC(6/5)
BfaI 332 CTAG C^TAG
BpmI 672 CTGGAG CTGGAG(16/14)
BpuAI 151 GAAGAC GAAGAC(2/6)
BsaAI 25 TACGTG [CT]AC^GT[GA]
BsgI 323 GTGCAG GTGCAG(16/14)
BsiWI 23 CGTACG C^GTACG
BsmAI 575 GAGAC GTCTC(1/5)
BsmFI 339 GTCCC GGGAC(10/14)
BsmI 465 GAATGC GAATGC(1/-1)
BsoBI 647 CTCGAG C^[CT]CG[GA]G
BspMI 488 GCAGGT ACCTGC(4/8)
BsrDI 351 CATTGC GCAATG(2/0)
BssKI 563 CCAGG ^CC[ACGT]GG
BstNI 563 CCAGG CC^[AT]GG
BstUI 14 CGCG CG^CG
BstXI 629 CCATTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 403 GATC ^GATC
DraI 766 TTTAAA TTT^AAA
EarI 513 CTCTTC CTCTTC(1/4)
EcoNI 263 CCTTTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 563 CCAGG ^CC[AT]GG
HgaI 12 GACGC GACGC(5/10)
HincII 684 GTTGAC GT[CT]^[GA]AC
HindII 684 GTTGAC GT[CT]^[GA]AC
HphI 843 TCACC GGTGA(8/7)
Ksp632I 513 CTCTTC CTCTTC(1/4)
MaeI 332 CTAG C^TAG
MaeII 26 ACGT A^CGT
MboI 403 GATC ^GATC
MslI 630 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 326 CAGCTG C[AC]G^C[GT]G
MvaI 563 CCAGG CC^[AT]GG
MvnI 14 CGCG CG^CG
MwoI 706 GCTCTGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 453 CATATG CA^TATG
NdeII 403 GATC ^GATC
NlaIV 307 GGAACC GG[ACGT]^[ACGT]CC
NspI 533 GCATGC [GA]CATG^[CT]
PaeR7I 647 CTCGAG C^TCGAG
PflMI 814 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 426 CTGCAG CTGCA^G
PvuII 326 CAGCTG CAG^CTG
SacI 704 GAGCTC GAGCT^C
Sau3AI 403 GATC ^GATC
ScrFI 563 CCAGG CC^[ACGT]GG
SfcI 426 CTGCAG C^T[GA][CT]AG
SmlI 647 CTCGAG C^T[CT][GA]AG
SphI 533 GCATGC GCATG^C
SspI 717 AATATT AAT^ATT
TfiI 825 GAATC G^A[AT]TC
Van91I 814 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 647 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 15 GCGG CCGC(-3/-1)
378 GCGG
AluI 327 AGCT AG^CT
705 AGCT
AspHI 40 GTGCTC G[AT]GC[AT]^C
704 GAGCTC
BmyI 40 GTGCTC G[AGT]GC[ACT]^C
704 GAGCTC
BseRI 107 GAGGAG GAGGAG(10/8)
781 GAGGAG
BsiHKAI 40 GTGCTC G[AT]GC[AT]^C
704 GAGCTC
BsiYI 263 CCTTTATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
814 CCAACAACTGG
BslI 263 CCTTTATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
814 CCAACAACTGG
Bsp1286I 40 GTGCTC G[AGT]GC[ACT]^C
704 GAGCTC
BstF5I 393 GGATG GGATG(2/0)
566 GGATG
Cac8I 54 GCTGGC GC[ACGT]^[ACGT]GC
533 GCATGC
Cfr13I 30 GGCCC G^G[ACGT]CC
639 GGGCC
FokI 393 GGATG GGATG(9/13)
566 GGATG
MaeIII 242 GTAAC ^GT[ACGT]AC
437 GTTAC
MseI 479 TTAA T^TAA
767 TTAA
Sau96I 30 GGCCC G^G[ACGT]CC
639 GGGCC
Tru9I 479 TTAA T^TAA
767 TTAA
TspRI 602 CACTG CA[GC]TG[ACGT][ACGT]^
670 CACTG
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AfaI BbvI BsrI Fnu4HI HaeIII
HinfI ItaI MboII MnlI NlaIII
PleI RsaI TaqI TseI Tsp509I
TthHB8I