Restriction map for
pFN21AB9932
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AlwI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MboI
MflI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI Sau3AI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI StuI SwaI Tth111I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1828 GTCTAC GT^[AC][GT]AC
AflII 1044 CTTAAG C^TTAAG
AlwNI 284 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 609 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 891 CCCGGG C^[CT]CG[GA]G
BanI 1247 GGCACC G^G[CT][GA]CC
BanII 1098 GAGCTC G[GA]GC[CT]^C
BfaI 1073 CTAG C^TAG
BfrI 1044 CTTAAG C^TTAAG
BsoBI 891 CCCGGG C^[CT]CG[GA]G
Bsp1407I 116 TGTACA T^GTACA
BspHI 1443 TCATGA T^CATGA
BsrDI 306 GCAATG GCAATG(2/0)
BsrGI 116 TGTACA T^GTACA
BstEII 1554 GGTAACC G^GT[ACGT]ACC
BstPI 1554 GGTAACC G^GT[ACGT]ACC
CfoI 1335 GCGC GCG^C
DraI 1353 TTTAAA TTT^AAA
DraII 1283 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1135 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1513 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1283 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1554 GGTAACC G^GT[ACGT]ACC
EcoRI 1489 GAATTC G^AATTC
EcoT14I 163 CCAAGG C^C[AT][AT]GG
FauI 1808 CCCGC CCCGC(4/6)
HaeIII 1205 GGCC GG^CC
HhaI 1335 GCGC GCG^C
HinP1I 1335 GCGC G^CGC
HincII 898 GTTGAC GT[CT]^[GA]AC
HindII 898 GTTGAC GT[CT]^[GA]AC
MaeI 1073 CTAG C^TAG
MaeII 551 ACGT A^CGT
MfeI 492 CAATTG C^AATTG
MspA1I 1788 CAGCTG C[AC]G^C[GT]G
MunI 492 CAATTG C^AATTG
NdeI 452 CATATG CA^TATG
PpuMI 1283 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1788 CAGCTG CAG^CTG
RcaI 1443 TCATGA T^CATGA
SacI 1098 GAGCTC GAGCT^C
SexAI 1821 ACCTGGT A^CC[AT]GGT
SmaI 891 CCCGGG CCC^GGG
SspBI 116 TGTACA T^GTACA
StyI 163 CCAAGG C^C[AT][AT]GG
XcmI 1299 CCACTGCTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 891 CCCGGG C^CCGGG
XmaI 891 CCCGGG C^CCGGG
XmnI 609 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 530 CCGC CCGC(-3/-1)
1809 CCGC
AcsI 884 AAATTT [GA]^AATT[CT]
1489 GAATTC
Alw44I 150 GTGCAC G^TGCAC
420 GTGCAC
ApaLI 150 GTGCAC G^TGCAC
420 GTGCAC
ApoI 884 AAATTT [GA]^AATT[CT]
1489 GAATTC
BbsI 1012 GAAGAC GAAGAC(2/6)
767 GTCTTC
BbvI 437 GCTGC GCAGC(8/12)
1294 GCTGC
BpmI 662 CTCCAG CTGGAG(16/14)
1465 CTCCAG
BpuAI 1012 GAAGAC GAAGAC(2/6)
767 GTCTTC
BsaWI 485 ACCGGA [AT]^CCGG[AT]
1558 ACCGGA
BsmI 406 GAATGC GAATGC(1/-1)
1639 GAATGC
EcoRV 1447 GATATC GAT^ATC
1652 GATATC
Fnu4HI 437 GCTGC GC^[ACGT]GC
1294 GCTGC
ItaI 437 GCTGC GC^[ACGT]GC
1294 GCTGC
MslI 966 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1249 CACCTGTGTG
PflMI 1544 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCAACGGATGG
PstI 1222 CTGCAG CTGCA^G
1321 CTGCAG
SbfI 1221 CCTGCAGG CCTGCA^GG
1320 CCTGCAGG
SmlI 812 CTCAAG C^T[CT][GA]AG
1044 CTTAAG
Sse8387I 1221 CCTGCAGG CCTGCA^GG
1320 CCTGCAGG
SspI 1597 AATATT AAT^ATT
1615 AATATT
TseI 437 GCTGC G^C[AT]GC
1294 GCTGC
Van91I 1544 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCAACGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AfaI AluI AspHI AvaII BcnI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EarI EcoRII
FokI HapII HinfI HpaII HphI
Ksp632I MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I