Restriction map for
pFN21AE6489
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsiEI BsiWI BsmFI BspDI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MaeI MamI MflI MluI MluNI
MscI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1045 GTCTAC GT^[AC][GT]AC
AlwI 1549 GATCC GGATC(4/5)
AlwNI 392 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 318 CCTGAGG CC^T[ACGT]AGG
AseI 870 ATTAAT AT^TAAT
AsnI 870 ATTAAT AT^TAAT
AvaI 752 CTCGGG C^[CT]CG[GA]G
BanI 998 GGCACC G^G[CT][GA]CC
BbvCI 894 GCTGAGG CCTCAGC(-5/-2)
BseRI 1308 GAGGAG GAGGAG(10/8)
BsmBI 4 GAGACG CGTCTC(1/5)
BsoBI 752 CTCGGG C^[CT]CG[GA]G
Bsp1407I 375 TGTACA T^GTACA
BspMI 1465 GCAGGT ACCTGC(4/8)
BsrDI 832 GCAATG GCAATG(2/0)
BsrGI 375 TGTACA T^GTACA
BstAPI 803 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 737 GGTTACC G^GT[ACGT]ACC
BstNI 1451 CCAGG CC^[AT]GG
BstPI 737 GGTTACC G^GT[ACGT]ACC
BstXI 917 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 318 CCTGAGG CC^T[ACGT]AGG
DpnII 1549 GATC ^GATC
DraI 982 TTTAAA TTT^AAA
DrdI 1710 GACAGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 221 TGGCCG [CT]^GGCC[GA]
Eco81I 318 CCTGAGG CC^T[ACGT]AGG
EcoO65I 737 GGTTACC G^GT[ACGT]ACC
EcoRII 1451 CCAGG ^CC[AT]GG
EcoT14I 106 CCTTGG C^C[AT][AT]GG
FauI 22 CCCGC CCCGC(4/6)
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
HindIII 122 AAGCTT A^AGCTT
MaeII 1377 ACGT A^CGT
MboI 1549 GATC ^GATC
MfeI 176 CAATTG C^AATTG
MunI 176 CAATTG C^AATTG
MvaI 1451 CCAGG CC^[AT]GG
NdeI 112 CATATG CA^TATG
NdeII 1549 GATC ^GATC
PflMI 391 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 870 ATTAAT AT^TAAT
PstI 347 CTGCAG CTGCA^G
PvuII 395 CAGCTG CAG^CTG
Sau3AI 1549 GATC ^GATC
SmlI 618 CTCAAG C^T[CT][GA]AG
SspBI 375 TGTACA T^GTACA
StyI 106 CCTTGG C^C[AT][AT]GG
Van91I 391 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 917 CCAAATTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 884 TCCGGA T^CCGGA
959 TCCGGA
AcyI 6 GACGCC G[GA]^CG[CT]C
1671 GGCGTC
AflIII 729 ACATGT A^C[GA][CT]GT
1589 ACATGT
Asp700I 120 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
574 GAAGAATTTC
AspHI 1446 GAGCAC G[AT]GC[AT]^C
1731 GAGCAC
BbsI 187 GAAGAC GAAGAC(2/6)
426 GAAGAC
BmyI 1446 GAGCAC G[AGT]GC[ACT]^C
1731 GAGCAC
BpuAI 187 GAAGAC GAAGAC(2/6)
426 GAAGAC
BsaHI 6 GACGCC G[GA]^CG[CT]C
1671 GGCGTC
BsaI 1160 GAGACC GGTCTC(1/5)
400 GGTCTC
BsaJI 106 CCTTGG C^C[ACGT][ACGT]GG
751 CCTCGG
BseAI 884 TCCGGA T^CCGGA
959 TCCGGA
BsiHKAI 1446 GAGCAC G[AT]GC[AT]^C
1731 GAGCAC
BsmI 970 GAATGC GAATGC(1/-1)
1399 GAATGC
Bsp1286I 1446 GAGCAC G[AGT]GC[ACT]^C
1731 GAGCAC
BspEI 884 TCCGGA T^CCGGA
959 TCCGGA
BspHI 134 TCATGA T^CATGA
1509 TCATGA
BspLU11I 729 ACATGT A^CATGT
1589 ACATGT
BsrI 91 CCAGT ACTGG(1/-1)
1134 CCAGT
EcoT22I 499 ATGCAT ATGCA^T
503 ATGCAT
HgaI 6 GACGC GACGC(5/10)
1672 GCGTC
Hin1I 6 GACGCC G[GA]^CG[CT]C
1671 GGCGTC
MroI 884 TCCGGA T^CCGGA
959 TCCGGA
MslI 174 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1376 CACGTCAGTG
MspA1I 13 CCGCTG C[AC]G^C[GT]G
395 CAGCTG
NsiI 499 ATGCAT ATGCA^T
503 ATGCAT
RcaI 134 TCATGA T^CATGA
1509 TCATGA
SphI 25 GCATGC GCATG^C
501 GCATGC
SspI 758 AATATT AAT^ATT
1682 AATATT
TaqI 56 TCGA T^CGA
596 TCGA
TthHB8I 56 TCGA T^CGA
596 TCGA
XmnI 120 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
574 GAAGAATTTC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BcnI BsaWI BsgI
BsiYI BslI BsmAI BssKI BstF5I
Cac8I Cfr13I DdeI EarI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MnlI MseI MspI MwoI NciI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI