Restriction map for
pFN21AE4021
##################################
# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BfrI BglI
BglII BlnI BmyI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AciI 588 CCGC CCGC(-3/-1)
AvaI 594 CCCGGG C^[CT]CG[GA]G
BbvI 406 GCTGC GCAGC(8/12)
BcgI 606 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 369 TGATCA T^GATCA
BlpI 232 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 232 GCTAAGC GC^T[ACGT]AGC
BsmFI 47 GTCCC GGGAC(10/14)
BsoBI 594 CCCGGG C^[CT]CG[GA]G
BstXI 50 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 135 GGATCT [GA]^GATC[CT]
Cac8I 40 GCTAGC GC[ACGT]^[ACGT]GC
CelII 232 GCTAAGC GC^T[ACGT]AGC
DdeI 233 CTAAG C^T[ACGT]AG
DsaI 448 CCGTGG C^C[GA][CT]GG
EcoNI 413 CCTATCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 349 CCAAGG C^C[AT][AT]GG
FbaI 369 TGATCA T^GATCA
Fnu4HI 406 GCTGC GC^[ACGT]GC
HapII 595 CCGG C^CGG
HpaII 595 CCGG C^CGG
ItaI 406 GCTGC GC^[ACGT]GC
MflI 135 GGATCT [GA]^GATC[CT]
MspI 595 CCGG C^CGG
NheI 40 GCTAGC G^CTAGC
NlaIV 622 GGCTCC GG[ACGT]^[ACGT]CC
NspI 282 GCATGT [GA]CATG^[CT]
PleI 529 GAGTC GAGTC(4/5)
SmaI 594 CCCGGG CCC^GGG
StyI 349 CCAAGG C^C[AT][AT]GG
TfiI 607 GATTC G^A[AT]TC
TseI 406 GCTGC G^C[AT]GC
XcmI 497 CCAGGAAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 135 GGATCT [GA]^GATC[CT]
XmaCI 594 CCCGGG C^CCGGG
XmaI 594 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 4 GAATTT [GA]^AATT[CT]
190 AAATTT
AlwI 135 GGATC GGATC(4/5)
598 GGATC
ApoI 4 GAATTT [GA]^AATT[CT]
190 AAATTT
BcnI 594 CCCGG CC^[GC]GG
595 CCGGG
BfaI 41 CTAG C^TAG
334 CTAG
BsgI 354 GTGCAG GTGCAG(16/14)
555 GTGCAG
BsmAI 107 GTCTC GTCTC(1/5)
531 GTCTC
Cfr13I 15 GGCCC G^G[ACGT]CC
392 GGCCC
HaeIII 15 GGCC GG^CC
392 GGCC
HinfI 529 GAGTC G^A[ACGT]TC
607 GATTC
HphI 372 TCACC GGTGA(8/7)
542 TCACC
MaeI 41 CTAG C^TAG
334 CTAG
MspA1I 87 CAGCTG C[AC]G^C[GT]G
208 CAGCTG
MwoI 158 GCCCCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
167 GCCACTTTTGC
NciI 594 CCCGG CC^[GC]GG
595 CCGGG
NlaIII 142 CATG CATG^
283 CATG
PvuII 87 CAGCTG CAG^CTG
208 CAGCTG
Sau96I 15 GGCCC G^G[ACGT]CC
392 GGCCC
SfaNI 116 GCATC GCATC(5/9)
378 GCATC
Tsp45I 79 GTGAC ^GT[GC]AC
541 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BpmI BsaJI BsiYI BslI
BsrI BssKI BstNI DpnII EarI
EcoRII Ksp632I MaeIII MboI MboII
MnlI MslI MvaI NdeII Sau3AI
ScrFI Tsp509I TspRI