Restriction map for
pFN21AE2007
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfaI BglII BlnI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I CpoI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 42 CTTAAG C^TTAAG
AflIII 546 ACATGT A^C[GA][CT]GT
AlwI 78 GGATC GGATC(4/5)
Asp700I 274 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1034 GAGCAC G[AT]GC[AT]^C
AvaII 396 GGACC G^G[AT]CC
BalI 1685 TGGCCA TGG^CCA
BanI 169 GGCACC G^G[CT][GA]CC
BciVI 1196 GGATAC GTATCC(6/5)
BfrI 42 CTTAAG C^TTAAG
BglI 448 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 98 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 98 GCTTAGC GC^T[ACGT]AGC
BsaAI 1231 TACGTG [CT]AC^GT[GA]
BsiHKAI 1034 GAGCAC G[AT]GC[AT]^C
BspDI 1552 ATCGAT AT^CGAT
BspLU11I 546 ACATGT A^CATGT
BstBI 1004 TTCGAA TT^CGAA
BstEII 613 GGTTACC G^GT[ACGT]ACC
BstPI 613 GGTTACC G^GT[ACGT]ACC
BstYI 78 GGATCT [GA]^GATC[CT]
CelII 98 GCTTAGC GC^T[ACGT]AGC
ClaI 1552 ATCGAT AT^CGAT
DraI 300 TTTAAA TTT^AAA
DraII 1578 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 1685 TGGCCA [CT]^GGCC[GA]
EcoO109I 1578 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 613 GGTTACC G^GT[ACGT]ACC
HincII 257 GTCAAC GT[CT]^[GA]AC
HindII 257 GTCAAC GT[CT]^[GA]AC
MflI 78 GGATCT [GA]^GATC[CT]
MluNI 1685 TGGCCA TGG^CCA
MscI 1685 TGGCCA TGG^CCA
MspA1I 429 CAGCTG C[AC]G^C[GT]G
NspV 1004 TTCGAA TT^CGAA
PflMI 202 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1611 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 215 CTGCAG CTGCA^G
PvuII 429 CAGCTG CAG^CTG
SfcI 215 CTGCAG C^T[GA][CT]AG
SfuI 1004 TTCGAA TT^CGAA
SphI 772 GCATGC GCATG^C
StuI 1723 AGGCCT AGG^CCT
Van91I 202 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 78 GGATCT [GA]^GATC[CT]
XmnI 274 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 542 GTCTAC GT^[AC][GT]AC
1189 GTAGAC
BpmI 432 CTGGAG CTGGAG(16/14)
1574 CTGGAG
BsmI 995 GAATGC GAATGC(1/-1)
812 GCATTC
BspMI 1059 GCAGGT ACCTGC(4/8)
1531 GCAGGT
BstAPI 217 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
880 GCAGGCTTTGC
Cfr13I 396 GGACC G^G[ACGT]CC
1579 GGCCC
DpnII 79 GATC ^GATC
517 GATC
DsaI 148 CCATGG C^C[GA][CT]GG
1054 CCATGG
EcoT22I 159 ATGCAT ATGCA^T
1398 ATGCAT
HindIII 4 AAGCTT A^AGCTT
240 AAGCTT
MboI 79 GATC ^GATC
517 GATC
NcoI 148 CCATGG C^CATGG
1054 CCATGG
NdeI 417 CATATG CA^TATG
1538 CATATG
NdeII 79 GATC ^GATC
517 GATC
NsiI 159 ATGCAT ATGCA^T
1398 ATGCAT
PleI 1570 GACTC GAGTC(4/5)
1439 GAGTC
Sau3AI 79 GATC ^GATC
517 GATC
Sau96I 396 GGACC G^G[ACGT]CC
1579 GGCCC
TaqI 1005 TCGA T^CGA
1553 TCGA
Tsp45I 58 GTCAC ^GT[GC]AC
1609 GTGAC
TthHB8I 1005 TCGA T^CGA
1553 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BmyI BpuAI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboII MnlI
MseI MslI MvaI MwoI NlaIII
NlaIV NspI RsaI ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI