Restriction map for
pFN21AE2460
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseAI BsmBI BsoBI Bsp1407I BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
CpoI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NruI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 820 GGTACC G^GTACC
Asp718I 820 GGTACC G^GTACC
AspHI 1009 GAGCTC G[AT]GC[AT]^C
BalI 1548 TGGCCA TGG^CCA
BcnI 290 CCCGG CC^[GC]GG
BfaI 542 CTAG C^TAG
BglII 264 AGATCT A^GATCT
BsaAI 701 TACGTG [CT]AC^GT[GA]
BsaWI 1054 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1009 GAGCTC G[AT]GC[AT]^C
BsiWI 699 CGTACG C^GTACG
BsmAI 344 GTCTC GTCTC(1/5)
BspDI 93 ATCGAT AT^CGAT
BspHI 636 TCATGA T^CATGA
BsrDI 552 GCAATG GCAATG(2/0)
BstAPI 1072 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 738 CCAGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 264 AGATCT [GA]^GATC[CT]
CfoI 16 GCGC GCG^C
ClaI 93 ATCGAT AT^CGAT
DraI 828 TTTAAA TTT^AAA
DraIII 1459 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 527 GAAGAG CTCTTC(1/4)
EcoNI 1090 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1426 ATGCAT ATGCA^T
HaeII 15 GGCGCT [GA]GCGC^[CT]
HgaI 1601 GCGTC GACGC(5/10)
HhaI 16 GCGC GCG^C
HinP1I 16 GCGC G^CGC
KpnI 820 GGTACC GGTAC^C
Ksp632I 527 GAAGAG CTCTTC(1/4)
MaeI 542 CTAG C^TAG
MflI 264 AGATCT [GA]^GATC[CT]
MluNI 1548 TGGCCA TGG^CCA
MscI 1548 TGGCCA TGG^CCA
MspA1I 1076 CAGCTG C[AC]G^C[GT]G
NciI 290 CCCGG CC^[GC]GG
NotI 49 GCGGCCGC GC^GGCCGC
NsiI 1426 ATGCAT ATGCA^T
PleI 1211 GAGTC GAGTC(4/5)
PstI 43 CTGCAG CTGCA^G
PvuII 1076 CAGCTG CAG^CTG
RcaI 636 TCATGA T^CATGA
SacI 1009 GAGCTC GAGCT^C
SapI 527 GAAGAGC GCTCTTC(1/4)
SbfI 42 CCTGCAGG CCTGCA^GG
ScaI 874 AGTACT AGT^ACT
SfaNI 188 GATGC GCATC(5/9)
SmlI 381 CTCAAG C^T[CT][GA]AG
Sse8387I 42 CCTGCAGG CCTGCA^GG
SspI 1191 AATATT AAT^ATT
StuI 925 AGGCCT AGG^CCT
TfiI 858 GATTC G^A[AT]TC
XcmI 1275 CCATGGACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 264 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 1240 GATCC GGATC(4/5)
1292 GGATC
AspI 161 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1359 GACAGTGTC
BanII 143 GGGCTC G[GA]GC[CT]^C
1009 GAGCTC
BbsI 891 GAAGAC GAAGAC(2/6)
1606 GTCTTC
BciVI 733 GGATAC GTATCC(6/5)
28 GTATCC
BpuAI 891 GAAGAC GAAGAC(2/6)
1606 GTCTTC
BseRI 1145 GAGGAG GAGGAG(10/8)
1208 GAGGAG
BsiEI 50 CGGCCG CG[GA][CT]^CG
1416 CGGCCG
BsmI 649 GAATGC GAATGC(1/-1)
1428 GCATTC
EagI 50 CGGCCG C^GGCCG
1416 CGGCCG
EclXI 50 CGGCCG C^GGCCG
1416 CGGCCG
Eco52I 50 CGGCCG C^GGCCG
1416 CGGCCG
HincII 559 GTCAAC GT[CT]^[GA]AC
1108 GTTGAC
HindII 559 GTCAAC GT[CT]^[GA]AC
1108 GTTGAC
HinfI 858 GATTC G^A[ACGT]TC
1211 GAGTC
MaeII 698 ACGT A^CGT
702 ACGT
MseI 434 TTAA T^TAA
829 TTAA
PflFI 161 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1359 GACAGTGTC
PflMI 460 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1339 CCAGCAGGTGG
Tru9I 434 TTAA T^TAA
829 TTAA
Tth111I 161 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1359 GACAGTGTC
Van91I 460 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1339 CCAGCAGGTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I