Restriction map for
pFN21AE6467
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstPI BstUI CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 833 GTATAC GT^[AC][GT]AC
AciI 1373 CCGC CCGC(-3/-1)
AfaI 1327 GTAC GT^AC
AflIII 432 ACATGT A^C[GA][CT]GT
AlwNI 593 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 824 CCTCAGG CC^T[ACGT]AGG
AseI 750 ATTAAT AT^TAAT
AsnI 750 ATTAAT AT^TAAT
Asp700I 733 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1451 GAGCTC G[AT]GC[AT]^C
AspI 240 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1451 GAGCTC G[GA]GC[CT]^C
BclI 1433 TGATCA T^GATCA
BmyI 1451 GAGCTC G[AGT]GC[ACT]^C
BpmI 1025 CTGGAG CTGGAG(16/14)
BsiHKAI 1451 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1451 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 432 ACATGT A^CATGT
Bst1107I 833 GTATAC GTA^TAC
BstBI 1232 TTCGAA TT^CGAA
BstXI 1002 CCAACGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1021 GGATCT [GA]^GATC[CT]
BstZ17I 833 GTATAC GTA^TAC
Bsu36I 824 CCTCAGG CC^T[ACGT]AGG
DrdI 1065 GACAAAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 824 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1 ATGCAT ATGCA^T
FbaI 1433 TGATCA T^GATCA
HaeIII 1475 GGCC GG^CC
HincII 1527 GTCAAC GT[CT]^[GA]AC
HindII 1527 GTCAAC GT[CT]^[GA]AC
MaeIII 387 GTTAC ^GT[ACGT]AC
MflI 1021 GGATCT [GA]^GATC[CT]
MspA1I 593 CAGCTG C[AC]G^C[GT]G
NdeI 1391 CATATG CA^TATG
NheI 760 GCTAGC G^CTAGC
NsiI 1 ATGCAT ATGCA^T
NspV 1232 TTCGAA TT^CGAA
PflFI 240 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1341 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 750 ATTAAT AT^TAAT
PstI 1519 CTGCAG CTGCA^G
PvuII 593 CAGCTG CAG^CTG
RsaI 1327 GTAC GT^AC
SacI 1451 GAGCTC GAGCT^C
SexAI 241 ACCTGGT A^CC[AT]GGT
SfaNI 1311 GATGC GCATC(5/9)
SfuI 1232 TTCGAA TT^CGAA
SmlI 807 CTTGAG C^T[CT][GA]AG
SpeI 1041 ACTAGT A^CTAGT
TspRI 789 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 240 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1353 TCTAGA T^CTAGA
XcmI 1208 CCACTCTAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1021 GGATCT [GA]^GATC[CT]
XmnI 733 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbsI 928 GAAGAC GAAGAC(2/6)
456 GTCTTC
BpuAI 928 GAAGAC GAAGAC(2/6)
456 GTCTTC
BsaJI 270 CCCTGG C^C[ACGT][ACGT]GG
628 CCCTGG
BseRI 62 CTCCTC GAGGAG(10/8)
182 GAGGAG
BsgI 92 CTGCAC GTGCAG(16/14)
590 GTGCAG
BsmI 737 GCATTC GAATGC(1/-1)
1466 GCATTC
BsrI 390 ACTGG ACTGG(1/-1)
790 ACTGG
Cac8I 760 GCTAGC GC[ACGT]^[ACGT]GC
882 GCTGGC
DraII 1341 AGGTCCT [GA]G^G[ACGT]CC[CT]
1473 GGGGCCT
EarI 374 CTCTTC CTCTTC(1/4)
951 GAAGAG
EcoO109I 1341 AGGTCCT [GA]G^G[ACGT]CC[CT]
1473 GGGGCCT
Ksp632I 374 CTCTTC CTCTTC(1/4)
951 GAAGAG
MaeII 866 ACGT A^CGT
1005 ACGT
NspI 432 ACATGT [GA]CATG^[CT]
802 ACATGC
SspI 1186 AATATT AAT^ATT
1290 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BfaI BsiYI BslI BsmAI
BsmFI BsrDI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoNI EcoRII
Fnu4HI FokI HinfI ItaI MaeI
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I