##################################
# NO CUT ENZYMES (  225 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcsI        AcyI        AfaI        AflII       
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AclI          350  AACGTT               AA^CGTT
AflIII        217  ACATGT               A^C[GA][CT]GT
BbsI          366  GAAGAC               GAAGAC(2/6)
BpuAI         366  GAAGAC               GAAGAC(2/6)
BsaJI         111  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI         107  CCAACCAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          107  CCAACCAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         115  GGGAC                GGGAC(10/14)
BspLU11I      217  ACATGT               A^CATGT
BsrDI         189  CATTGC               GCAATG(2/0)
BsrI           70  CCAGT                ACTGG(1/-1)
BstBI         338  TTCGAA               TT^CGAA
EcoT14I       111  CCAAGG               C^C[AT][AT]GG
HinfI         232  GATTC                G^A[ACGT]TC
MaeII         351  ACGT                 A^CGT
MnlI           24  CCTC                 CCTC(7/6)
NlaIII        218  CATG                 CATG^
NlaIV          64  GGAACC               GG[ACGT]^[ACGT]CC
NspI          217  ACATGT               [GA]CATG^[CT]
NspV          338  TTCGAA               TT^CGAA
Psp1406I      350  AACGTT               AA^CGTT
SfuI          338  TTCGAA               TT^CGAA
StyI          111  CCAAGG               C^C[AT][AT]GG
TaqI          339  TCGA                 T^CGA
TfiI          232  GATTC                G^A[AT]TC
TspRI         197  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       339  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

MseI          146  TTAA                 T^TAA
              257  TTAA                
MwoI           89  GCTGTTCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              164  GCCCTTCCTGC         
SfaNI          32  GCATC                GCATC(5/9)
               98  GCATC               
Tru9I         146  TTAA                 T^TAA
              257  TTAA                
Tsp509I       152  AATT                 ^AATT
              180  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MboII