Restriction map for
pFN21AE1604
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# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DpnII DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluI MroI
MslI MspA1I MunI NarI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 152 GAATTC [GA]^AATT[CT]
AluI 249 AGCT AG^CT
AlwNI 139 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 152 GAATTC [GA]^AATT[CT]
AvaII 196 GGACC G^G[AT]CC
BalI 110 TGGCCA TGG^CCA
BsmAI 254 GTCTC GTCTC(1/5)
BsmFI 189 GTCCC GGGAC(10/14)
BsrFI 86 GCCGGC [GA]^CCGG[CT]
BsrI 129 CCAGT ACTGG(1/-1)
BssHII 90 GCGCGC G^CGCGC
BstAPI 138 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 168 CATCC GGATG(2/0)
Cfr10I 86 GCCGGC [GA]^CCGG[CT]
DsaI 272 CCATGG C^C[GA][CT]GG
EcoRI 152 GAATTC G^AATTC
EcoT14I 272 CCATGG C^C[AT][AT]GG
FokI 168 CATCC GGATG(9/13)
HindIII 248 AAGCTT A^AGCTT
MluNI 110 TGGCCA TGG^CCA
MnlI 159 CCTC CCTC(7/6)
MscI 110 TGGCCA TGG^CCA
MseI 278 TTAA T^TAA
NaeI 86 GCCGGC GCC^GGC
NcoI 272 CCATGG C^CATGG
NgoMIV 86 GCCGGC G^CCGGC
NlaIII 273 CATG CATG^
PstI 145 CTGCAG CTGCA^G
SbfI 144 CCTGCAGG CCTGCA^GG
SfcI 145 CTGCAG C^T[GA][CT]AG
Sse8387I 144 CCTGCAGG CCTGCA^GG
StyI 272 CCATGG C^C[AT][AT]GG
TaqI 290 TCGA T^CGA
Tru9I 278 TTAA T^TAA
Tsp509I 153 AATT ^AATT
TthHB8I 290 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 7 CGCG CG^CG
91 CGCG
BcnI 38 CCCGG CC^[GC]GG
193 CCCGG
BstNI 72 CCTGG CC^[AT]GG
108 CCTGG
BstUI 7 CGCG CG^CG
91 CGCG
Cfr13I 3 GGCCC G^G[ACGT]CC
196 GGACC
EaeI 77 TGGCCG [CT]^GGCC[GA]
110 TGGCCA
EcoRII 72 CCTGG ^CC[AT]GG
108 CCTGG
HaeII 54 GGCGCT [GA]GCGC^[CT]
102 AGCGCC
HphI 183 GGTGA GGTGA(8/7)
156 TCACC
MvaI 72 CCTGG CC^[AT]GG
108 CCTGG
MvnI 7 CGCG CG^CG
91 CGCG
NciI 38 CCCGG CC^[GC]GG
193 CCCGG
NlaIV 41 GGCTCC GG[ACGT]^[ACGT]CC
196 GGACCC
Sau96I 3 GGCCC G^G[ACGT]CC
196 GGACC
SfaNI 297 GATGC GCATC(5/9)
265 GCATC
SmlI 160 CTCAAG C^T[CT][GA]AG
232 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI Cac8I CfoI FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MspI MwoI ScrFI TseI
TspRI