Restriction map for
pFN21AE6371
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I FbaI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KspI MaeII MamI MfeI MluI
MluNI MscI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NgoMIV NotI NruI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1157 GGTACC G^GTACC
AccIII 779 TCCGGA T^CCGGA
AcsI 295 GAATTT [GA]^AATT[CT]
AcyI 1048 GACGCC G[GA]^CG[CT]C
ApoI 295 GAATTT [GA]^AATT[CT]
AseI 664 ATTAAT AT^TAAT
AsnI 664 ATTAAT AT^TAAT
Asp718I 1157 GGTACC G^GTACC
AspHI 950 GAGCAC G[AT]GC[AT]^C
BamHI 387 GGATCC G^GATCC
BanI 1157 GGTACC G^G[CT][GA]CC
BbsI 144 GAAGAC GAAGAC(2/6)
BmyI 950 GAGCAC G[AGT]GC[ACT]^C
BpmI 1118 CTGGAG CTGGAG(16/14)
BpuAI 144 GAAGAC GAAGAC(2/6)
BsaHI 1048 GACGCC G[GA]^CG[CT]C
BsaI 775 GGTCTC GGTCTC(1/5)
BsaWI 779 TCCGGA [AT]^CCGG[AT]
BseAI 779 TCCGGA T^CCGGA
BsiHKAI 950 GAGCAC G[AT]GC[AT]^C
BsiYI 624 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 624 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 950 GAGCAC G[AGT]GC[ACT]^C
BspEI 779 TCCGGA T^CCGGA
BstBI 759 TTCGAA TT^CGAA
DraII 450 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 10 CGGCCA [CT]^GGCC[GA]
EarI 595 GAAGAG CTCTTC(1/4)
EcoO109I 450 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1150 GATATC GAT^ATC
FauI 1239 GCGGG CCCGC(4/6)
HapII 780 CCGG C^CGG
Hin1I 1048 GACGCC G[GA]^CG[CT]C
HincII 326 GTCAAC GT[CT]^[GA]AC
HindII 326 GTCAAC GT[CT]^[GA]AC
HindIII 1037 AAGCTT A^AGCTT
HpaII 780 CCGG C^CGG
KpnI 1157 GGTACC GGTAC^C
Ksp632I 595 GAAGAG CTCTTC(1/4)
MroI 779 TCCGGA T^CCGGA
MspI 780 CCGG C^CGG
NdeI 352 CATATG CA^TATG
NheI 619 GCTAGC G^CTAGC
NspI 1102 GCATGC [GA]CATG^[CT]
NspV 759 TTCGAA TT^CGAA
PacI 661 TTAATTAA TTAAT^TAA
PpuMI 450 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 664 ATTAAT AT^TAAT
PstI 625 CTGCAG CTGCA^G
SexAI 918 ACCAGGT A^CC[AT]GGT
SfuI 759 TTCGAA TT^CGAA
SmlI 1034 CTCAAG C^T[CT][GA]AG
SphI 1102 GCATGC GCATG^C
TfiI 250 GAATC G^A[AT]TC
XcmI 1107 CCAGGGTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 533 GCGG CCGC(-3/-1)
1239 GCGG
AfaI 877 GTAC GT^AC
1158 GTAC
AvaII 451 GGACC G^G[AT]CC
1123 GGACC
BsmFI 583 GGGAC GGGAC(10/14)
1241 GGGAC
BsrDI 526 CATTGC GCAATG(2/0)
1025 GCAATG
BstF5I 902 CATCC GGATG(2/0)
633 GGATG
BstYI 387 GGATCC [GA]^GATC[CT]
465 AGATCC
FokI 902 CATCC GGATG(9/13)
633 GGATG
HgaI 1012 GACGC GACGC(5/10)
1048 GACGC
MflI 387 GGATCC [GA]^GATC[CT]
465 AGATCC
MslI 643 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
820 CATGACTATG
RsaI 877 GTAC GT^AC
1158 GTAC
TaqI 760 TCGA T^CGA
987 TCGA
TthHB8I 760 TCGA T^CGA
987 TCGA
XhoII 387 GGATCC [GA]^GATC[CT]
465 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AlwI BbvI BfaI BsaJI
BsmAI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT22I
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
NsiI PleI Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI