Restriction map for
pFN21AE9457
##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
ApaI AscI AseI AsnI Asp700I
AspI AviII AvrII BcgI BglII
BlnI BpmI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssSI Bst1107I
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
Eco81I FseI FspI HpaI MfeI
MluI MroI MunI NdeI NotI
NruI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3907 GGTACC G^GTACC
AccI 1644 GTCTAC GT^[AC][GT]AC
AflII 2854 CTTAAG C^TTAAG
AflIII 1494 ACGTGT A^C[GA][CT]GT
Alw44I 3496 GTGCAC G^TGCAC
Aor51HI 66 AGCGCT AGC^GCT
ApaLI 3496 GTGCAC G^TGCAC
Asp718I 3907 GGTACC G^GTACC
BalI 3854 TGGCCA TGG^CCA
BbrPI 3460 CACGTG CAC^GTG
BbvCI 3406 CCTCAGC CCTCAGC(-5/-2)
BclI 807 TGATCA T^GATCA
BfrI 2854 CTTAAG C^TTAAG
BlpI 1758 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1758 GCTGAGC GC^T[ACGT]AGC
BsaAI 3460 CACGTG [CT]AC^GT[GA]
BsaBI 2361 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3173 TCATGA T^CATGA
BspMI 213 GCAGGT ACCTGC(4/8)
BsrBI 52 CCGCTC CCGCTC(-3/-3)
BssHII 29 GCGCGC G^CGCGC
BstAPI 213 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3485 TTCGAA TT^CGAA
CelII 1758 GCTGAGC GC^T[ACGT]AGC
EarI 3662 GAAGAG CTCTTC(1/4)
Eco47III 66 AGCGCT AGC^GCT
EcoNI 1529 CCTGCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 547 GAATTC G^AATTC
EcoRV 276 GATATC GAT^ATC
EcoT22I 592 ATGCAT ATGCA^T
FbaI 807 TGATCA T^GATCA
KpnI 3907 GGTACC GGTAC^C
Ksp632I 3662 GAAGAG CTCTTC(1/4)
KspI 107 CCGCGG CCGC^GG
MamI 2361 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3854 TGGCCA TGG^CCA
MscI 3854 TGGCCA TGG^CCA
NcoI 1140 CCATGG C^CATGG
NheI 1575 GCTAGC G^CTAGC
NsiI 592 ATGCAT ATGCA^T
NspV 3485 TTCGAA TT^CGAA
PaeR7I 3651 CTCGAG C^TCGAG
PmaCI 3460 CACGTG CAC^GTG
PmlI 3460 CACGTG CAC^GTG
RcaI 3173 TCATGA T^CATGA
SacII 107 CCGCGG CCGC^GG
SapI 3662 GAAGAGC GCTCTTC(1/4)
SexAI 634 ACCAGGT A^CC[AT]GGT
SfuI 3485 TTCGAA TT^CGAA
SgrAI 16 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3778 ACTAGT A^CTAGT
XhoI 3651 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 2064 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3548 GACTCACAGTC
AspEI 2064 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3548 GACTCACAGTC
AvaI 146 CCCGAG C^[CT]CG[GA]G
3651 CTCGAG
BamHI 1731 GGATCC G^GATCC
2586 GGATCC
BbsI 1550 GTCTTC GAAGAC(2/6)
3285 GTCTTC
BciVI 630 GGATAC GTATCC(6/5)
2648 GTATCC
BglI 17 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3626 GCCGGCTGGGC
BpuAI 1550 GTCTTC GAAGAC(2/6)
3285 GTCTTC
BsoBI 146 CCCGAG C^[CT]CG[GA]G
3651 CTCGAG
BstEII 249 GGTCACC G^GT[ACGT]ACC
1995 GGTTACC
BstPI 249 GGTCACC G^GT[ACGT]ACC
1995 GGTTACC
DraII 215 AGGTCCT [GA]G^G[ACGT]CC[CT]
3894 GGGACCC
Eam1105I 2064 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3548 GACTCACAGTC
EcoO109I 215 AGGTCCT [GA]G^G[ACGT]CC[CT]
3894 GGGACCC
EcoO65I 249 GGTCACC G^GT[ACGT]ACC
1995 GGTTACC
PpuMI 215 AGGTCCT [GA]G^G[AT]CC[CT]
3894 GGGACCC
PvuII 1756 CAGCTG CAG^CTG
3806 CAGCTG
SacI 1586 GAGCTC GAGCT^C
3195 GAGCTC
ScaI 1040 AGTACT AGT^ACT
2517 AGTACT
SspI 937 AATATT AAT^ATT
1615 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BmyI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII