Restriction map for
pFN21AE2003
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# NO CUT ENZYMES ( 131 enzymes) #
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Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT22I FauI
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MseI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tru9I Tth111I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 44 CGCG CG^CG
AccIII 977 TCCGGA T^CCGGA
AfaI 437 GTAC GT^AC
Asp700I 1132 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 7 CTCGAG C^[CT]CG[GA]G
BanI 1059 GGCACC G^G[CT][GA]CC
BglII 1608 AGATCT A^GATCT
BsaWI 977 TCCGGA [AT]^CCGG[AT]
BseAI 977 TCCGGA T^CCGGA
BsgI 123 GTGCAG GTGCAG(16/14)
BsmBI 193 CGTCTC CGTCTC(1/5)
BsoBI 7 CTCGAG C^[CT]CG[GA]G
BspEI 977 TCCGGA T^CCGGA
BsrBI 1316 GAGCGG CCGCTC(-3/-3)
BsrDI 1728 GCAATG GCAATG(2/0)
BstUI 44 CGCG CG^CG
DraIII 1336 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1112 GACTTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1694 CCATGG C^C[GA][CT]GG
EcoRV 1438 GATATC GAT^ATC
EcoT14I 1694 CCATGG C^C[AT][AT]GG
HgaI 45 GCGTC GACGC(5/10)
HindIII 1595 AAGCTT A^AGCTT
MroI 977 TCCGGA T^CCGGA
MslI 792 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 44 CGCG CG^CG
NcoI 1694 CCATGG C^CATGG
NspI 1379 ACATGC [GA]CATG^[CT]
PaeR7I 7 CTCGAG C^TCGAG
PflMI 1526 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1667 GACTC GAGTC(4/5)
PshAI 1692 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 437 GTAC GT^AC
SacI 4 GAGCTC GAGCT^C
SmlI 7 CTCGAG C^T[CT][GA]AG
SspI 949 AATATT AAT^ATT
StyI 1694 CCATGG C^C[AT][AT]GG
Van91I 1526 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1611 TCTAGA T^CTAGA
XhoI 7 CTCGAG C^TCGAG
XmnI 1132 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 191 GACGTC GACGT^C
1429 GACGTC
AccI 925 GTATAC GT^[AC][GT]AC
1086 GTATAC
AcsI 244 GAATTC [GA]^AATT[CT]
710 GAATTC
AcyI 191 GACGTC G[GA]^CG[CT]C
1429 GACGTC
ApoI 244 GAATTC [GA]^AATT[CT]
710 GAATTC
AspHI 4 GAGCTC G[AT]GC[AT]^C
787 GTGCTC
AvaII 288 GGACC G^G[AT]CC
1698 GGTCC
BalI 20 TGGCCA TGG^CCA
998 TGGCCA
BanII 4 GAGCTC G[GA]GC[CT]^C
1387 GAGCCC
BbsI 188 GAAGAC GAAGAC(2/6)
1489 GAAGAC
BpuAI 188 GAAGAC GAAGAC(2/6)
1489 GAAGAC
BsaHI 191 GACGTC G[GA]^CG[CT]C
1429 GACGTC
BsiHKAI 4 GAGCTC G[AT]GC[AT]^C
787 GTGCTC
BsrI 120 CCAGT ACTGG(1/-1)
1703 CCAGT
Bst1107I 925 GTATAC GTA^TAC
1086 GTATAC
BstZ17I 925 GTATAC GTA^TAC
1086 GTATAC
CfoI 43 GCGC GCG^C
605 GCGC
Cfr13I 288 GGACC G^G[ACGT]CC
1698 GGTCC
EaeI 20 TGGCCA [CT]^GGCC[GA]
998 TGGCCA
EcoRI 244 GAATTC G^AATTC
710 GAATTC
HaeIII 21 GGCC GG^CC
999 GGCC
HhaI 43 GCGC GCG^C
605 GCGC
Hin1I 191 GACGTC G[GA]^CG[CT]C
1429 GACGTC
HinP1I 43 GCGC G^CGC
605 GCGC
MluNI 20 TGGCCA TGG^CCA
998 TGGCCA
MscI 20 TGGCCA TGG^CCA
998 TGGCCA
Sau96I 288 GGACC G^G[ACGT]CC
1698 GGTCC
Tsp45I 170 GTGAC ^GT[GC]AC
376 GTGAC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI Sau3AI ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp509I
TspRI TthHB8I XhoII