Restriction map for
pFN21AE2070
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbsI BbvCI BciVI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AocI 712 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2027 AGCGCT AGC^GCT
ApaI 1451 GGGCCC GGGCC^C
Asp700I 1935 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1833 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 84 TGGCCA TGG^CCA
BamHI 1472 GGATCC G^GATCC
BbrPI 1974 CACGTG CAC^GTG
BcgI 544 CGAGTGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1895 CTAG C^TAG
BglI 503 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 353 TCCGGT [AT]^CCGG[AT]
BspLU11I 1937 ACATGT A^CATGT
BsrFI 1771 GCCGGC [GA]^CCGG[CT]
Bst1107I 639 GTATAC GTA^TAC
BstAPI 1566 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 639 GTATAC GTA^TAC
Bsu36I 712 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1771 GCCGGC [GA]^CCGG[CT]
Eco47III 2027 AGCGCT AGC^GCT
Eco81I 712 CCTGAGG CC^T[ACGT]AGG
EcoNI 39 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2027 AGCGCT [GA]GCGC^[CT]
HindIII 175 AAGCTT A^AGCTT
MaeI 1895 CTAG C^TAG
MluNI 84 TGGCCA TGG^CCA
MscI 84 TGGCCA TGG^CCA
NaeI 1771 GCCGGC GCC^GGC
NcoI 924 CCATGG C^CATGG
NgoMIV 1771 GCCGGC G^CCGGC
PflFI 1833 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1974 CACGTG CAC^GTG
PmlI 1974 CACGTG CAC^GTG
SacI 624 GAGCTC GAGCT^C
SexAI 251 ACCAGGT A^CC[AT]GGT
StuI 2043 AGGCCT AGG^CCT
Tth111I 1833 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1935 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1937 ACATGT A^C[GA][CT]GT
1973 ACACGT
BanI 717 GGTGCC G^G[CT][GA]CC
1872 GGTGCC
BclI 587 TGATCA T^GATCA
1130 TGATCA
BlpI 1366 GCTGAGC GC^T[ACGT]AGC
1601 GCTCAGC
BpmI 652 CTGGAG CTGGAG(16/14)
867 CTGGAG
Bpu1102I 1366 GCTGAGC GC^T[ACGT]AGC
1601 GCTCAGC
BsaAI 1974 CACGTG [CT]AC^GT[GA]
2119 TACGTG
BsaI 280 GAGACC GGTCTC(1/5)
2109 GGTCTC
BsgI 552 GTGCAG GTGCAG(16/14)
1669 GTGCAG
BsmI 631 GAATGC GAATGC(1/-1)
2071 GAATGC
BsrBI 1320 CCGCTC CCGCTC(-3/-3)
1908 CCGCTC
BstYI 89 AGATCC [GA]^GATC[CT]
1472 GGATCC
CelII 1366 GCTGAGC GC^T[ACGT]AGC
1601 GCTCAGC
CfoI 1497 GCGC GCG^C
2028 GCGC
DraIII 58 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
2153 CACAAAGTG
EarI 565 CTCTTC CTCTTC(1/4)
1378 CTCTTC
FauI 1319 CCCGC CCCGC(4/6)
1907 CCCGC
FbaI 587 TGATCA T^GATCA
1130 TGATCA
HgaI 1465 GCGTC GACGC(5/10)
1925 GCGTC
HhaI 1497 GCGC GCG^C
2028 GCGC
HinP1I 1497 GCGC G^CGC
2028 GCGC
HincII 670 GTCAAC GT[CT]^[GA]AC
1594 GTCAAC
HindII 670 GTCAAC GT[CT]^[GA]AC
1594 GTCAAC
Ksp632I 565 CTCTTC CTCTTC(1/4)
1378 CTCTTC
MflI 89 AGATCC [GA]^GATC[CT]
1472 GGATCC
MslI 405 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1515 CACCACGGTG
SmlI 1729 CTTGAG C^T[CT][GA]AG
2019 CTTGAG
XhoII 89 AGATCC [GA]^GATC[CT]
1472 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI