##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstBI       BstEII      BstNI       BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DpnII       DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MboI        MboII       MfeI        MflI        
MluI        MroI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tsp45I      Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AfaI            8  GTAC                 GT^AC
AseI          192  ATTAAT               AT^TAAT
AsnI          192  ATTAAT               AT^TAAT
BalI            2  TGGCCA               TGG^CCA
BsaBI         238  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI          33  CATTGC               GCAATG(2/0)
BsrI            5  CCAGT                ACTGG(1/-1)
BstAPI         43  GCAGGATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        268  CATCC                GGATG(2/0)
BstXI           5  CCAGTACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          261  TTTAAA               TTT^AAA
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoNI         271  CCTGACAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          268  CATCC                GGATG(9/13)
MaeII          62  ACGT                 A^CGT
MaeIII         59  GTTAC                ^GT[ACGT]AC
MamI          238  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
NdeI           82  CATATG               CA^TATG
NlaIII         75  CATG                 CATG^
NlaIV          87  GGAGCC               GG[ACGT]^[ACGT]CC
PshBI         192  ATTAAT               AT^TAAT
PstI           41  CTGCAG               CTGCA^G
RsaI            8  GTAC                 GT^AC
SfaNI         238  GATGC                GCATC(5/9)
SfcI           41  CTGCAG               C^T[GA][CT]AG
SmlI           92  CTCAAG               C^T[CT][GA]AG
TaqI          246  TCGA                 T^CGA
Tsp509I       252  AATT                 ^AATT
TthHB8I       246  TCGA                 T^CGA
XcmI           74  CCATGAAGCATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI          315  AGCT                 AG^CT
              339  AGCT                
BfaI          199  CTAG                 C^TAG
              328  CTAG                
BsiYI         218  CCAATAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              271  CCTGACAAAGG         
BslI          218  CCAATAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              271  CCTGACAAAGG         
Cac8I          52  GCAGGC               GC[ACGT]^[ACGT]GC
               69  GCAAGC              
HaeIII          3  GGCC                 GG^CC
               55  GGCC                
MaeI          199  CTAG                 C^TAG
              328  CTAG                
MseI          193  TTAA                 T^TAA
              262  TTAA                
MwoI           43  GCAGGATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               81  GCATATGGAGC         
Tru9I         193  TTAA                 T^TAA
              262  TTAA                
TspRI          11  CAGTG                CA[GC]TG[ACGT][ACGT]^
              135  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BbvI        Fnu4HI      ItaI        MnlI        TseI