Restriction map for
pFN21AE3690
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Aor51HI ApaI
AscI Asp718I AviII AvrII BanI
BbeI BcgI BciVI BcnI BglI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FseI FspI HaeII Hin1I HindIII
HphI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI Sse8387I StuI Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 74 enzymes) #
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AflII 2529 CTTAAG C^TTAAG
AflIII 2325 ACATGT A^C[GA][CT]GT
AhdI 1780 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2321 GTGCAC G^TGCAC
AlwNI 2300 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2189 CCTCAGG CC^T[ACGT]AGG
ApaLI 2321 GTGCAC G^TGCAC
Asp700I 2671 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1780 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2840 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2771 CTCGAG C^[CT]CG[GA]G
BalI 324 TGGCCA TGG^CCA
BbrPI 2450 CACGTG CAC^GTG
BbsI 2175 GTCTTC GAAGAC(2/6)
BbvCI 697 GCTGAGG CCTCAGC(-5/-2)
BclI 1433 TGATCA T^GATCA
BfrI 2529 CTTAAG C^TTAAG
BlpI 1696 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1696 GCTCAGC GC^T[ACGT]AGC
BpuAI 2175 GTCTTC GAAGAC(2/6)
BseRI 1411 GAGGAG GAGGAG(10/8)
BsiYI 681 CCTTGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 681 CCTTGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 285 GAATGC GAATGC(1/-1)
BsoBI 2771 CTCGAG C^[CT]CG[GA]G
BspHI 1602 TCATGA T^CATGA
BspLU11I 2325 ACATGT A^CATGT
BsrDI 912 CATTGC GCAATG(2/0)
BsrFI 1548 ACCGGC [GA]^CCGG[CT]
Bsu36I 2189 CCTCAGG CC^T[ACGT]AGG
CelII 1696 GCTCAGC GC^T[ACGT]AGC
CfoI 2506 GCGC GCG^C
Cfr10I 1548 ACCGGC [GA]^CCGG[CT]
DraII 513 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 2315 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 324 TGGCCA [CT]^GGCC[GA]
Eam1105I 1780 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2189 CCTCAGG CC^T[ACGT]AGG
EcoO109I 513 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1102 GATATC GAT^ATC
FauI 1148 CCCGC CCCGC(4/6)
FbaI 1433 TGATCA T^GATCA
HapII 1549 CCGG C^CGG
HhaI 2506 GCGC GCG^C
HinP1I 2506 GCGC G^CGC
HincII 1000 GTTAAC GT[CT]^[GA]AC
HindII 1000 GTTAAC GT[CT]^[GA]AC
HpaI 1000 GTTAAC GTT^AAC
HpaII 1549 CCGG C^CGG
MluNI 324 TGGCCA TGG^CCA
MscI 324 TGGCCA TGG^CCA
MspA1I 695 CAGCTG C[AC]G^C[GT]G
MspI 1549 CCGG C^CGG
NdeI 439 CATATG CA^TATG
PaeR7I 2771 CTCGAG C^TCGAG
PflFI 2840 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2450 CACGTG CAC^GTG
PmlI 2450 CACGTG CAC^GTG
PpuMI 513 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 695 CAGCTG CAG^CTG
RcaI 1602 TCATGA T^CATGA
SacI 2253 GAGCTC GAGCT^C
SapI 566 GAAGAGC GCTCTTC(1/4)
ScaI 35 AGTACT AGT^ACT
SnaBI 1584 TACGTA TAC^GTA
SpeI 148 ACTAGT A^CTAGT
SphI 597 GCATGC GCATG^C
SwaI 504 ATTTAAAT ATTT^AAAT
TaqI 2772 TCGA T^CGA
Tth111I 2840 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2772 TCGA T^CGA
XbaI 1072 TCTAGA T^CTAGA
XhoI 2771 CTCGAG C^TCGAG
XmnI 2671 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 2253 GAGCTC G[AT]GC[AT]^C
2321 GTGCAC
BamHI 21 GGATCC G^GATCC
2787 GGATCC
BanII 558 GGGCTC G[GA]GC[CT]^C
2253 GAGCTC
BpmI 131 CTCCAG CTGGAG(16/14)
2435 CTCCAG
BsaAI 1584 TACGTA [CT]AC^GT[GA]
2450 CACGTG
BsaI 1416 GAGACC GGTCTC(1/5)
1787 GGTCTC
BsgI 518 CTGCAC GTGCAG(16/14)
1379 GTGCAG
BsiHKAI 2253 GAGCTC G[AT]GC[AT]^C
2321 GTGCAC
BsmAI 1416 GAGAC GTCTC(1/5)
1788 GTCTC
BsmFI 2083 GGGAC GGGAC(10/14)
1899 GTCCC
Bsp1407I 1254 TGTACA T^GTACA
1500 TGTACA
BspMI 346 GCAGGT ACCTGC(4/8)
2737 GCAGGT
BsrGI 1254 TGTACA T^GTACA
1500 TGTACA
BstAPI 412 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2095 GCACATTATGC
BstXI 781 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2835 CCATAGACTTGG
DsaI 861 CCATGG C^C[GA][CT]GG
2648 CCATGG
EcoRI 112 GAATTC G^AATTC
1767 GAATTC
EcoT14I 861 CCATGG C^C[AT][AT]GG
2648 CCATGG
HgaI 1066 GACGC GACGC(5/10)
1854 GCGTC
NcoI 861 CCATGG C^CATGG
2648 CCATGG
PleI 1009 GACTC GAGTC(4/5)
2887 GACTC
PstI 602 CTGCAG CTGCA^G
2762 CTGCAG
SfaNI 2831 GATGC GCATC(5/9)
1069 GCATC
SmlI 2529 CTTAAG C^T[CT][GA]AG
2771 CTCGAG
SspBI 1254 TGTACA T^GTACA
1500 TGTACA
SspI 1591 AATATT AAT^ATT
1912 AATATT
StyI 861 CCATGG C^C[AT][AT]GG
2648 CCATGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BbvI
BfaI BmyI BsaJI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PshBI RsaI Sau3AI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII