Restriction map for
pFN21AE3709
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BlnI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaI BseAI BsiEI
BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MboII MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 144 GACGCC G[GA]^CG[CT]C
AlwI 219 GATCC GGATC(4/5)
Aor51HI 382 AGCGCT AGC^GCT
BclI 248 TGATCA T^GATCA
BglI 73 GCCATTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 631 CTGGAG CTGGAG(16/14)
BsaAI 495 CACGTA [CT]AC^GT[GA]
BsaHI 144 GACGCC G[GA]^CG[CT]C
BsaWI 150 TCCGGT [AT]^CCGG[AT]
BsgI 257 GTGCAG GTGCAG(16/14)
BsmAI 142 GAGAC GTCTC(1/5)
BsmBI 142 GAGACG CGTCTC(1/5)
BsmFI 132 GTCCC GGGAC(10/14)
BsmI 225 GCATTC GAATGC(1/-1)
BspLU11I 534 ACATGT A^CATGT
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BssKI 620 CCAGG ^CC[ACGT]GG
BstNI 620 CCAGG CC^[AT]GG
BstXI 444 CCACAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 135 CCGTGG C^C[GA][CT]GG
Eco47III 382 AGCGCT AGC^GCT
EcoRII 620 CCAGG ^CC[AT]GG
EcoT22I 24 ATGCAT ATGCA^T
FauI 30 CCCGC CCCGC(4/6)
FbaI 248 TGATCA T^GATCA
HaeII 382 AGCGCT [GA]GCGC^[CT]
HgaI 144 GACGC GACGC(5/10)
Hin1I 144 GACGCC G[GA]^CG[CT]C
MaeII 496 ACGT A^CGT
MslI 445 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 620 CCAGG CC^[AT]GG
NlaIV 109 GGGGCC GG[ACGT]^[ACGT]CC
NsiI 24 ATGCAT ATGCA^T
PflMI 620 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 173 GAGTC GAGTC(4/5)
PpuMI 92 AGGACCT [GA]G^G[AT]CC[CT]
ScrFI 620 CCAGG CC^[ACGT]GG
SmlI 412 CTCAAG C^T[CT][GA]AG
TaqI 350 TCGA T^CGA
Tsp45I 440 GTGAC ^GT[GC]AC
Tsp509I 573 AATT ^AATT
TspRI 103 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 350 TCGA T^CGA
Van91I 620 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 620 CCAGGGTTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AflIII 494 ACACGT A^C[GA][CT]GT
534 ACATGT
AvaII 93 GGACC G^G[AT]CC
577 GGTCC
BglII 303 AGATCT A^GATCT
521 AGATCT
BseRI 262 GAGGAG GAGGAG(10/8)
343 GAGGAG
CfoI 82 GCGC GCG^C
383 GCGC
DraII 92 AGGACCT [GA]G^G[ACGT]CC[CT]
125 AGGCCCT
EcoO109I 92 AGGACCT [GA]G^G[ACGT]CC[CT]
125 AGGCCCT
HapII 34 CCGG C^CGG
151 CCGG
HhaI 82 GCGC GCG^C
383 GCGC
HinP1I 82 GCGC G^CGC
383 GCGC
HpaII 34 CCGG C^CGG
151 CCGG
MspI 34 CCGG C^CGG
151 CCGG
NspI 22 ACATGC [GA]CATG^[CT]
534 ACATGT
TfiI 291 GAATC G^A[AT]TC
464 GAATC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsrI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MflI MnlI MseI MwoI
NdeII NlaIII Sau3AI Sau96I SfaNI
Tru9I TseI XhoII