Restriction map for
pFN21AE1782
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1286I BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII ItaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 339 CCGC CCGC(-3/-1)
AflII 463 CTTAAG C^TTAAG
AflIII 232 ACATGT A^C[GA][CT]GT
AhdI 325 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 154 GATCC GGATC(4/5)
AlwNI 518 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 325 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI 166 CTAG C^TAG
BfrI 463 CTTAAG C^TTAAG
BsaJI 84 CCATGG C^C[ACGT][ACGT]GG
BseRI 717 GAGGAG GAGGAG(10/8)
BsiYI 60 CCCATATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 60 CCCATATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 123 GAGAC GTCTC(1/5)
BsmI 169 GAATGC GAATGC(1/-1)
Bsp1407I 105 TGTACA T^GTACA
BspLU11I 232 ACATGT A^CATGT
BsrDI 8 CATTGC GCAATG(2/0)
BsrGI 105 TGTACA T^GTACA
BstYI 153 AGATCC [GA]^GATC[CT]
DdeI 144 CTTAG C^T[ACGT]AG
DpnII 154 GATC ^GATC
DsaI 84 CCATGG C^C[GA][CT]GG
Eam1105I 325 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 271 GAAGAG CTCTTC(1/4)
EcoT14I 84 CCATGG C^C[AT][AT]GG
EcoT22I 701 ATGCAT ATGCA^T
HaeIII 197 GGCC GG^CC
HinfI 94 GAATC G^A[ACGT]TC
HphI 459 TCACC GGTGA(8/7)
Ksp632I 271 GAAGAG CTCTTC(1/4)
MaeI 166 CTAG C^TAG
MboI 154 GATC ^GATC
MflI 153 AGATCC [GA]^GATC[CT]
MspA1I 339 CCGCTG C[AC]G^C[GT]G
MwoI 12 GCCTGCCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 84 CCATGG C^CATGG
NdeI 708 CATATG CA^TATG
NdeII 154 GATC ^GATC
NsiI 701 ATGCAT ATGCA^T
NspI 232 ACATGT [GA]CATG^[CT]
Sau3AI 154 GATC ^GATC
ScaI 507 AGTACT AGT^ACT
SexAI 446 ACCTGGT A^CC[AT]GGT
SspBI 105 TGTACA T^GTACA
StuI 196 AGGCCT AGG^CCT
StyI 84 CCATGG C^C[AT][AT]GG
TfiI 94 GAATC G^A[AT]TC
TspRI 311 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 639 CCATTCTGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 153 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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AfaI 106 GTAC GT^AC
508 GTAC
AluI 110 AGCT AG^CT
218 AGCT
BbsI 3 GAAGAC GAAGAC(2/6)
46 GTCTTC
BpuAI 3 GAAGAC GAAGAC(2/6)
46 GTCTTC
MaeIII 205 GTGAC ^GT[ACGT]AC
397 GTGAC
MnlI 199 CCTC CCTC(7/6)
717 GAGG
MslI 704 CATCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
708 CATATGTGTG
RsaI 106 GTAC GT^AC
508 GTAC
SmlI 463 CTTAAG C^T[CT][GA]AG
660 CTCAAG
Tsp45I 205 GTGAC ^GT[GC]AC
397 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AcsI ApoI BsrI BssKI BstF5I
BstNI Cac8I EcoRII FokI MboII
MseI MvaI NlaIII ScrFI SfaNI
Tru9I Tsp509I