################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TseI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 339 CCGC CCGC(-3/-1) AflII 463 CTTAAG C^TTAAG AflIII 232 ACATGT A^C[GA][CT]GT AhdI 325 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 154 GATCC GGATC(4/5) AlwNI 518 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 325 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BfaI 166 CTAG C^TAG BfrI 463 CTTAAG C^TTAAG BsaJI 84 CCATGG C^C[ACGT][ACGT]GG BseRI 717 GAGGAG GAGGAG(10/8) BsiYI 60 CCCATATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 60 CCCATATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 123 GAGAC GTCTC(1/5) BsmI 169 GAATGC GAATGC(1/-1) Bsp1407I 105 TGTACA T^GTACA BspLU11I 232 ACATGT A^CATGT BsrDI 8 CATTGC GCAATG(2/0) BsrGI 105 TGTACA T^GTACA BstYI 153 AGATCC [GA]^GATC[CT] DdeI 144 CTTAG C^T[ACGT]AG DpnII 154 GATC ^GATC DsaI 84 CCATGG C^C[GA][CT]GG Eam1105I 325 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 271 GAAGAG CTCTTC(1/4) EcoT14I 84 CCATGG C^C[AT][AT]GG EcoT22I 701 ATGCAT ATGCA^T HaeIII 197 GGCC GG^CC HinfI 94 GAATC G^A[ACGT]TC HphI 459 TCACC GGTGA(8/7) Ksp632I 271 GAAGAG CTCTTC(1/4) MaeI 166 CTAG C^TAG MboI 154 GATC ^GATC MflI 153 AGATCC [GA]^GATC[CT] MspA1I 339 CCGCTG C[AC]G^C[GT]G MwoI 12 GCCTGCCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 84 CCATGG C^CATGG NdeI 708 CATATG CA^TATG NdeII 154 GATC ^GATC NsiI 701 ATGCAT ATGCA^T NspI 232 ACATGT [GA]CATG^[CT] Sau3AI 154 GATC ^GATC ScaI 507 AGTACT AGT^ACT SexAI 446 ACCTGGT A^CC[AT]GGT SspBI 105 TGTACA T^GTACA StuI 196 AGGCCT AGG^CCT StyI 84 CCATGG C^C[AT][AT]GG TfiI 94 GAATC G^A[AT]TC TspRI 311 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 639 CCATTCTGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 153 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 106 GTAC GT^AC 508 GTAC AluI 110 AGCT AG^CT 218 AGCT BbsI 3 GAAGAC GAAGAC(2/6) 46 GTCTTC BpuAI 3 GAAGAC GAAGAC(2/6) 46 GTCTTC MaeIII 205 GTGAC ^GT[ACGT]AC 397 GTGAC MnlI 199 CCTC CCTC(7/6) 717 GAGG MslI 704 CATCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 708 CATATGTGTG RsaI 106 GTAC GT^AC 508 GTAC SmlI 463 CTTAAG C^T[CT][GA]AG 660 CTCAAG Tsp45I 205 GTGAC ^GT[GC]AC 397 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AcsI ApoI BsrI BssKI BstF5I BstNI Cac8I EcoRII FokI MboII MseI MvaI NlaIII ScrFI SfaNI Tru9I Tsp509I