Restriction map for
pFN21AE6034
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanII BbrPI BbvCI
BcgI BciVI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FseI FspI
HapII HgaI HindIII HpaI HpaII
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MspI
MunI MvaI MvnI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AcyI 439 GGCGCC G[GA]^CG[CT]C
AflII 900 CTTAAG C^TTAAG
AlwI 757 GGATC GGATC(4/5)
Asp700I 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 371 TGGCCA TGG^CCA
BanI 439 GGCGCC G^G[CT][GA]CC
BbeI 439 GGCGCC GGCGC^C
BbsI 69 GAAGAC GAAGAC(2/6)
BclI 107 TGATCA T^GATCA
BfrI 900 CTTAAG C^TTAAG
BpuAI 69 GAAGAC GAAGAC(2/6)
BsaHI 439 GGCGCC G[GA]^CG[CT]C
BsgI 290 GTGCAG GTGCAG(16/14)
Bsp1407I 176 TGTACA T^GTACA
BspHI 401 TCATGA T^CATGA
BsrGI 176 TGTACA T^GTACA
BsrI 588 ACTGG ACTGG(1/-1)
BstF5I 725 CATCC GGATG(2/0)
BstYI 757 GGATCT [GA]^GATC[CT]
CfoI 440 GCGC GCG^C
Cfr13I 701 GGGCC G^G[ACGT]CC
DsaI 113 CCGTGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EarI 542 CTCTTC CTCTTC(1/4)
EcoT14I 689 CCAAGG C^C[AT][AT]GG
FbaI 107 TGATCA T^GATCA
FokI 725 CATCC GGATG(9/13)
HaeII 439 GGCGCC [GA]GCGC^[CT]
HhaI 440 GCGC GCG^C
Hin1I 439 GGCGCC G[GA]^CG[CT]C
HinP1I 440 GCGC G^CGC
HincII 568 GTTGAC GT[CT]^[GA]AC
HindII 568 GTTGAC GT[CT]^[GA]AC
KasI 439 GGCGCC G^GCGCC
Ksp632I 542 CTCTTC CTCTTC(1/4)
MflI 757 GGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 818 CAGCTG C[AC]G^C[GT]G
NarI 439 GGCGCC GG^CGCC
NlaIV 439 GGCGCC GG[ACGT]^[ACGT]CC
PvuII 818 CAGCTG CAG^CTG
RcaI 401 TCATGA T^CATGA
Sau96I 701 GGGCC G^G[ACGT]CC
SfaNI 724 GCATC GCATC(5/9)
SfoI 439 GGCGCC GGC^GCC
SspBI 176 TGTACA T^GTACA
SspI 124 AATATT AAT^ATT
StyI 689 CCAAGG C^C[AT][AT]GG
XhoII 757 GGATCT [GA]^GATC[CT]
XmnI 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 786 AAATTT [GA]^AATT[CT]
908 AAATTC
ApoI 786 AAATTT [GA]^AATT[CT]
908 AAATTC
BbvI 346 GCAGC GCAGC(8/12)
820 GCTGC
BsaJI 113 CCGTGG C^C[ACGT][ACGT]GG
689 CCAAGG
BsiYI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BslI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BsmI 422 GCATTC GAATGC(1/-1)
462 GCATTC
Fnu4HI 346 GCAGC GC^[ACGT]GC
820 GCTGC
HphI 57 TCACC GGTGA(8/7)
110 TCACC
ItaI 346 GCAGC GC^[ACGT]GC
820 GCTGC
MseI 643 TTAA T^TAA
901 TTAA
MwoI 346 GCAGCAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
442 GCCTTCATAGC
PleI 72 GACTC GAGTC(4/5)
557 GACTC
PstI 326 CTGCAG CTGCA^G
388 CTGCAG
SfcI 326 CTGCAG C^T[GA][CT]AG
388 CTGCAG
SmlI 900 CTTAAG C^T[CT][GA]AG
929 CTCAAG
Tru9I 643 TTAA T^TAA
901 TTAA
TseI 346 GCAGC G^C[AT]GC
820 GCTGC
Tsp509I 787 AATT ^AATT
909 AATT
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AfaI AluI DdeI DpnII HaeIII
HinfI MboI MboII MnlI MslI
NdeII NlaIII RsaI Sau3AI TfiI
TspRI