Restriction map for
pFN21AE6072
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 346 GCGG CCGC(-3/-1)
AcsI 919 AAATTT [GA]^AATT[CT]
AlwI 610 GGATC GGATC(4/5)
ApoI 919 AAATTT [GA]^AATT[CT]
BalI 371 TGGCCA TGG^CCA
BclI 107 TGATCA T^GATCA
BsaI 509 GGTCTC GGTCTC(1/5)
BsgI 782 GTGCAG GTGCAG(16/14)
BsiYI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 510 GTCTC GTCTC(1/5)
BsmI 422 GCATTC GAATGC(1/-1)
Bsp1407I 176 TGTACA T^GTACA
BspMI 458 ACCTGC ACCTGC(4/8)
BsrDI 349 GCAATG GCAATG(2/0)
BsrGI 176 TGTACA T^GTACA
BssKI 778 CCAGG ^CC[ACGT]GG
BstNI 778 CCAGG CC^[AT]GG
Cac8I 451 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 113 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoRII 778 CCAGG ^CC[AT]GG
FbaI 107 TGATCA T^GATCA
Fnu4HI 346 GCGGC GC^[ACGT]GC
HincII 495 GTTAAC GT[CT]^[GA]AC
HindII 495 GTTAAC GT[CT]^[GA]AC
HindIII 701 AAGCTT A^AGCTT
HpaI 495 GTTAAC GTT^AAC
HphI 110 TCACC GGTGA(8/7)
ItaI 346 GCGGC GC^[ACGT]GC
MaeII 159 ACGT A^CGT
MaeIII 926 GTCAC ^GT[ACGT]AC
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MvaI 778 CCAGG CC^[AT]GG
NcoI 113 CCATGG C^CATGG
NdeI 375 CATATG CA^TATG
NlaIV 439 GGAGCC GG[ACGT]^[ACGT]CC
ScrFI 778 CCAGG CC^[ACGT]GG
SexAI 777 ACCAGGT A^CC[AT]GGT
SspBI 176 TGTACA T^GTACA
TaqI 774 TCGA T^CGA
Tsp45I 926 GTCAC ^GT[GC]AC
TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 774 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 177 GTAC GT^AC
393 GTAC
BbsI 69 GAAGAC GAAGAC(2/6)
161 GTCTTC
BfaI 196 CTAG C^TAG
448 CTAG
BpuAI 69 GAAGAC GAAGAC(2/6)
161 GTCTTC
BsaJI 113 CCATGG C^C[ACGT][ACGT]GG
683 CCAAGG
DpnII 108 GATC ^GATC
611 GATC
EcoT14I 113 CCATGG C^C[AT][AT]GG
683 CCAAGG
HaeIII 354 GGCC GG^CC
372 GGCC
MaeI 196 CTAG C^TAG
448 CTAG
MboI 108 GATC ^GATC
611 GATC
MwoI 346 GCGGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
442 GCCTACCTAGC
NdeII 108 GATC ^GATC
611 GATC
PstI 388 CTGCAG CTGCA^G
434 CTGCAG
RsaI 177 GTAC GT^AC
393 GTAC
Sau3AI 108 GATC ^GATC
611 GATC
SfaNI 462 GCATC GCATC(5/9)
718 GCATC
StyI 113 CCATGG C^C[AT][AT]GG
683 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BstF5I DdeI EarI FokI
HinfI Ksp632I MboII MnlI MseI
MslI NlaIII SfcI SspI TfiI
Tru9I Tsp509I