Restriction map for
pFN21AE5918
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI ItaI
KasI KpnI KspI MaeI MaeII
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 192 GAATTT [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
ApaI 757 GGGCCC GGGCC^C
ApoI 192 GAATTT [GA]^AATT[CT]
AseI 432 ATTAAT AT^TAAT
AsnI 432 ATTAAT AT^TAAT
Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 725 GAGCTC G[AT]GC[AT]^C
BbsI 455 GTCTTC GAAGAC(2/6)
BclI 245 TGATCA T^GATCA
BpuAI 455 GTCTTC GAAGAC(2/6)
BsaBI 240 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 725 GAGCTC G[AT]GC[AT]^C
BsmAI 822 GTCTC GTCTC(1/5)
BsmFI 634 GGGAC GGGAC(10/14)
BspMI 607 GCAGGT ACCTGC(4/8)
BsrDI 20 CATTGC GCAATG(2/0)
BssKI 84 CCTGG ^CC[ACGT]GG
BstNI 84 CCTGG CC^[AT]GG
DraII 757 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 326 CCACGG C^C[GA][CT]GG
EarI 4 GAAGAG CTCTTC(1/4)
EcoO109I 757 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 84 CCTGG ^CC[AT]GG
EcoRV 273 GATATC GAT^ATC
EcoT14I 186 CCTTGG C^C[AT][AT]GG
FbaI 245 TGATCA T^GATCA
HaeIII 758 GGCC GG^CC
HapII 426 CCGG C^CGG
HpaII 426 CCGG C^CGG
HphI 110 TCACC GGTGA(8/7)
Ksp632I 4 GAAGAG CTCTTC(1/4)
MamI 240 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 426 CCGG C^CGG
MvaI 84 CCTGG CC^[AT]GG
MwoI 346 GCAACAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI 432 ATTAAT AT^TAAT
RsaI 177 GTAC GT^AC
SacI 725 GAGCTC GAGCT^C
ScrFI 84 CCTGG CC^[ACGT]GG
SfcI 867 CTACAG C^T[GA][CT]AG
StyI 186 CCTTGG C^C[AT][AT]GG
XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG
326 CCACGG
BsiYI 78 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCATCATGGGG
BslI 78 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCATCATGGGG
BsmI 286 GAATGC GAATGC(1/-1)
88 GCATTC
Cfr13I 757 GGGCC G^G[ACGT]CC
758 GGCCC
NlaIV 757 GGGCCC GG[ACGT]^[ACGT]CC
758 GGCCCC
PleI 231 GACTC GAGTC(4/5)
820 GAGTC
Sau96I 757 GGGCC G^G[ACGT]CC
758 GGCCC
TfiI 123 GAATC G^A[AT]TC
571 GATTC
Tsp45I 229 GTGAC ^GT[GC]AC
536 GTGAC
Tsp509I 193 AATT ^AATT
615 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BanII BmyI Bsp1286I BstF5I
DdeI DpnII FokI HinfI MaeIII
MboI MboII MnlI MseI MslI
NdeI NdeII NlaIII Sau3AI SfaNI
Tru9I TspRI