Restriction map for
pFN21AB9399
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BciVI BfrI
BglI BlnI BsaAI BsiEI BsiWI
BsmBI BspHI BsrFI BssHII BstUI
Cfr10I CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
FseI FspI HaeII HpaI KasI
KpnI KspI MluI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 3437 TCCGGA T^CCGGA
AcyI 2722 GGCGTC G[GA]^CG[CT]C
AocI 4419 CCTGAGG CC^T[ACGT]AGG
AspI 4081 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 852 CTCGAG C^[CT]CG[GA]G
BclI 2378 TGATCA T^GATCA
BglII 4097 AGATCT A^GATCT
BsaBI 90 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2722 GGCGTC G[GA]^CG[CT]C
BsaWI 3437 TCCGGA [AT]^CCGG[AT]
BseAI 3437 TCCGGA T^CCGGA
BsmI 2340 GAATGC GAATGC(1/-1)
BsoBI 852 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4793 TGTACA T^GTACA
BspEI 3437 TCCGGA T^CCGGA
BsrBI 37 GAGCGG CCGCTC(-3/-3)
BsrGI 4793 TGTACA T^GTACA
BstBI 5231 TTCGAA TT^CGAA
Bsu36I 4419 CCTGAGG CC^T[ACGT]AGG
DraIII 1565 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4419 CCTGAGG CC^T[ACGT]AGG
EcoRI 925 GAATTC G^AATTC
FauI 2297 GCGGG CCCGC(4/6)
FbaI 2378 TGATCA T^GATCA
Hin1I 2722 GGCGTC G[GA]^CG[CT]C
MamI 90 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 77 CAATTG C^AATTG
MroI 3437 TCCGGA T^CCGGA
MunI 77 CAATTG C^AATTG
NdeI 1205 CATATG CA^TATG
NspV 5231 TTCGAA TT^CGAA
PaeR7I 852 CTCGAG C^TCGAG
PflFI 4081 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2112 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1232 CTGCAG CTGCA^G
SfuI 5231 TTCGAA TT^CGAA
SspBI 4793 TGTACA T^GTACA
Tth111I 4081 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2679 CCAGGAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 852 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2900 ACATGT A^C[GA][CT]GT
4790 ACATGT
AseI 997 ATTAAT AT^TAAT
3622 ATTAAT
AsnI 997 ATTAAT AT^TAAT
3622 ATTAAT
BamHI 2937 GGATCC G^GATCC
4018 GGATCC
BbvCI 154 CCTCAGC CCTCAGC(-5/-2)
789 CCTCAGC
BcgI 2084 GCAACTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3839 GCAATTCACTCG
BlpI 3201 GCTGAGC GC^T[ACGT]AGC
4668 GCTCAGC
Bpu1102I 3201 GCTGAGC GC^T[ACGT]AGC
4668 GCTCAGC
BsaI 2674 GAGACC GGTCTC(1/5)
4069 GGTCTC
BspDI 2510 ATCGAT AT^CGAT
4582 ATCGAT
BspLU11I 2900 ACATGT A^CATGT
4790 ACATGT
CelII 3201 GCTGAGC GC^T[ACGT]AGC
4668 GCTCAGC
ClaI 2510 ATCGAT AT^CGAT
4582 ATCGAT
DrdI 595 GACTCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4297 GACTGTGTGGTC
HgaI 4377 GACGC GACGC(5/10)
2723 GCGTC
PpuMI 1703 AGGTCCT [GA]G^G[AT]CC[CT]
4844 AGGACCC
PshBI 997 ATTAAT AT^TAAT
3622 ATTAAT
SacI 214 GAGCTC GAGCT^C
2842 GAGCTC
SspI 531 AATATT AAT^ATT
4925 AATATT
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# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
Bst1107I BstAPI BstEII BstF5I BstNI
BstPI BstXI BstYI BstZ17I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI