Restriction map for
pFN21AE2442
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI BbrPI
BcgI BsiWI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI Eam1105I Eco47III
FseI HpaI KpnI KspI MfeI
MluI MunI NotI NruI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SgrAI SnaBI SwaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 3826 GGCGCC G[GA]^CG[CT]C
AspI 3568 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3422 TGCGCA TGC^GCA
BbeI 3826 GGCGCC GGCGC^C
BglII 618 AGATCT A^GATCT
BlpI 3489 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3489 GCTCAGC GC^T[ACGT]AGC
BsaAI 546 CACGTA [CT]AC^GT[GA]
BsaBI 241 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3826 GGCGCC G[GA]^CG[CT]C
BsgI 5599 CTGCAC GTGCAG(16/14)
BsiEI 4979 CGGCCG CG[GA][CT]^CG
BsmBI 3187 GAGACG CGTCTC(1/5)
BsrFI 4981 GCCGGC [GA]^CCGG[CT]
BssSI 387 CTCGTG CACGAG(-5/-1)
BstEII 4066 GGTTACC G^GT[ACGT]ACC
BstPI 4066 GGTTACC G^GT[ACGT]ACC
CelII 3489 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4981 GCCGGC [GA]^CCGG[CT]
DraI 4096 TTTAAA TTT^AAA
EagI 4979 CGGCCG C^GGCCG
EclXI 4979 CGGCCG C^GGCCG
Eco52I 4979 CGGCCG C^GGCCG
EcoNI 5205 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4066 GGTTACC G^GT[ACGT]ACC
EcoRI 4 GAATTC G^AATTC
EcoT22I 3354 ATGCAT ATGCA^T
FspI 3422 TGCGCA TGC^GCA
HaeII 3826 GGCGCC [GA]GCGC^[CT]
HgaI 584 GCGTC GACGC(5/10)
Hin1I 3826 GGCGCC G[GA]^CG[CT]C
KasI 3826 GGCGCC G^GCGCC
MamI 241 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4981 GCCGGC GCC^GGC
NarI 3826 GGCGCC GG^CGCC
NgoMIV 4981 GCCGGC G^CCGGC
NheI 1293 GCTAGC G^CTAGC
NsiI 3354 ATGCAT ATGCA^T
PflFI 3568 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 4710 GCTCTTC GCTCTTC(1/4)
SbfI 2727 CCTGCAGG CCTGCA^GG
SfiI 1630 GGCCAGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3826 GGCGCC GGC^GCC
SmaI 4136 CCCGGG CCC^GGG
SpeI 1720 ACTAGT A^CTAGT
Sse8387I 2727 CCTGCAGG CCTGCA^GG
SspI 3716 AATATT AAT^ATT
Tth111I 3568 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 243 TCTAGA T^CTAGA
XmaCI 4136 CCCGGG C^CCGGG
XmaI 4136 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 51 TCCGGA T^CCGGA
1274 TCCGGA
AcsI 4 GAATTC [GA]^AATT[CT]
4517 AAATTC
AflII 3607 CTTAAG C^TTAAG
4678 CTTAAG
ApoI 4 GAATTC [GA]^AATT[CT]
4517 AAATTC
AvrII 2424 CCTAGG C^CTAGG
5433 CCTAGG
BanI 3826 GGCGCC G^G[CT][GA]CC
5059 GGCACC
BciVI 3384 GGATAC GTATCC(6/5)
2531 GTATCC
BclI 353 TGATCA T^GATCA
4469 TGATCA
BfrI 3607 CTTAAG C^TTAAG
4678 CTTAAG
BlnI 2424 CCTAGG C^CTAGG
5433 CCTAGG
BseAI 51 TCCGGA T^CCGGA
1274 TCCGGA
Bsp1407I 4175 TGTACA T^GTACA
5147 TGTACA
BspEI 51 TCCGGA T^CCGGA
1274 TCCGGA
BsrGI 4175 TGTACA T^GTACA
5147 TGTACA
BstBI 2077 TTCGAA TT^CGAA
3746 TTCGAA
DraIII 721 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2013 CACCTTGTG
EcoRV 886 GATATC GAT^ATC
1537 GATATC
FbaI 353 TGATCA T^GATCA
4469 TGATCA
HindIII 3548 AAGCTT A^AGCTT
4427 AAGCTT
MroI 51 TCCGGA T^CCGGA
1274 TCCGGA
NdeI 599 CATATG CA^TATG
2592 CATATG
NspV 2077 TTCGAA TT^CGAA
3746 TTCGAA
PaeR7I 1264 CTCGAG C^TCGAG
1870 CTCGAG
SexAI 1160 ACCTGGT A^CC[AT]GGT
2633 ACCTGGT
SfuI 2077 TTCGAA TT^CGAA
3746 TTCGAA
SphI 3797 GCATGC GCATG^C
4949 GCATGC
SspBI 4175 TGTACA T^GTACA
5147 TGTACA
XhoI 1264 CTCGAG C^TCGAG
1870 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AvaI AvaII BalI BamHI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI