Restriction map for
pFN21AE1831
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaI BseAI BsmFI BsmI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco47III Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I MfeI MflI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tth111I XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 979 AAATTC [GA]^AATT[CT]
AflIII 32 ACGTGT A^C[GA][CT]GT
ApoI 979 AAATTC [GA]^AATT[CT]
AvaI 506 CTCGGG C^[CT]CG[GA]G
BanI 530 GGCGCC G^G[CT][GA]CC
BanII 286 GAGCTC G[GA]GC[CT]^C
BbeI 530 GGCGCC GGCGC^C
BpmI 592 CTGGAG CTGGAG(16/14)
BsaBI 201 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 581 ACCGGA [AT]^CCGG[AT]
BseRI 835 GAGGAG GAGGAG(10/8)
BsiWI 320 CGTACG C^GTACG
BsmBI 874 CGTCTC CGTCTC(1/5)
BsoBI 506 CTCGGG C^[CT]CG[GA]G
Bsp1407I 806 TGTACA T^GTACA
BspHI 536 TCATGA T^CATGA
BsrFI 559 GCCGGC [GA]^CCGG[CT]
BsrGI 806 TGTACA T^GTACA
BstAPI 1007 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 559 GCCGGC [GA]^CCGG[CT]
DdeI 382 CTGAG C^T[ACGT]AG
DpnII 513 GATC ^GATC
EcoNI 678 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 530 GGCGCC G^GCGCC
MamI 201 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 513 GATC ^GATC
NaeI 559 GCCGGC GCC^GGC
NarI 530 GGCGCC GG^CGCC
NcoI 788 CCATGG C^CATGG
NdeII 513 GATC ^GATC
NgoMIV 559 GCCGGC G^CCGGC
PflMI 956 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 159 GAGTC GAGTC(4/5)
RcaI 536 TCATGA T^CATGA
SacI 286 GAGCTC GAGCT^C
Sau3AI 513 GATC ^GATC
SexAI 800 ACCTGGT A^CC[AT]GGT
SfoI 530 GGCGCC GGC^GCC
SspBI 806 TGTACA T^GTACA
TaqI 479 TCGA T^CGA
TfiI 120 GATTC G^A[AT]TC
Tsp509I 980 AATT ^AATT
TthHB8I 479 TCGA T^CGA
Van91I 956 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 573 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 49 GGCGTC G[GA]^CG[CT]C
530 GGCGCC
AspHI 71 GAGCAC G[AT]GC[AT]^C
286 GAGCTC
AvaII 579 GGACC G^G[AT]CC
644 GGACC
AviII 755 TGCGCA TGC^GCA
770 TGCGCA
BcnI 488 CCCGG CC^[GC]GG
895 CCCGG
BfaI 130 CTAG C^TAG
574 CTAG
BsaHI 49 GGCGTC G[GA]^CG[CT]C
530 GGCGCC
BsgI 624 CTGCAC GTGCAG(16/14)
706 GTGCAG
BsiHKAI 71 GAGCAC G[AT]GC[AT]^C
286 GAGCTC
BsmAI 793 GAGAC GTCTC(1/5)
875 GTCTC
BstF5I 80 CATCC GGATG(2/0)
584 GGATG
DraIII 607 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
915 CACCATGTG
FauI 135 CCCGC CCCGC(4/6)
723 GCGGG
FokI 80 CATCC GGATG(9/13)
584 GGATG
FspI 755 TGCGCA TGC^GCA
770 TGCGCA
HaeII 491 GGCGCT [GA]GCGC^[CT]
530 GGCGCC
Hin1I 49 GGCGTC G[GA]^CG[CT]C
530 GGCGCC
HinfI 120 GATTC G^A[ACGT]TC
159 GAGTC
KspI 136 CCGCGG CCGC^GG
332 CCGCGG
MaeI 130 CTAG C^TAG
574 CTAG
NciI 488 CCCGG CC^[GC]GG
895 CCCGG
PstI 366 CTGCAG CTGCA^G
1014 CTGCAG
PvuII 589 CAGCTG CAG^CTG
766 CAGCTG
SacII 136 CCGCGG CCGC^GG
332 CCGCGG
SfcI 366 CTGCAG C^T[GA][CT]AG
1014 CTGCAG
Tsp45I 355 GTCAC ^GT[GC]AC
911 GTGAC
XcmI 606 CCACGGCGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
848 CCACGGGCAGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI BbsI
BbvI BmyI BpuAI BsaAI BsaJI
BsiEI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DsaI EaeI EagI
EclXI Eco52I EcoRII EcoT14I Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI StyI
TseI TspRI