################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaI BseAI BsmFI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 979 AAATTC [GA]^AATT[CT] AflIII 32 ACGTGT A^C[GA][CT]GT ApoI 979 AAATTC [GA]^AATT[CT] AvaI 506 CTCGGG C^[CT]CG[GA]G BanI 530 GGCGCC G^G[CT][GA]CC BanII 286 GAGCTC G[GA]GC[CT]^C BbeI 530 GGCGCC GGCGC^C BpmI 592 CTGGAG CTGGAG(16/14) BsaBI 201 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 581 ACCGGA [AT]^CCGG[AT] BseRI 835 GAGGAG GAGGAG(10/8) BsiWI 320 CGTACG C^GTACG BsmBI 874 CGTCTC CGTCTC(1/5) BsoBI 506 CTCGGG C^[CT]CG[GA]G Bsp1407I 806 TGTACA T^GTACA BspHI 536 TCATGA T^CATGA BsrFI 559 GCCGGC [GA]^CCGG[CT] BsrGI 806 TGTACA T^GTACA BstAPI 1007 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 559 GCCGGC [GA]^CCGG[CT] DdeI 382 CTGAG C^T[ACGT]AG DpnII 513 GATC ^GATC EcoNI 678 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG KasI 530 GGCGCC G^GCGCC MamI 201 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 513 GATC ^GATC NaeI 559 GCCGGC GCC^GGC NarI 530 GGCGCC GG^CGCC NcoI 788 CCATGG C^CATGG NdeII 513 GATC ^GATC NgoMIV 559 GCCGGC G^CCGGC PflMI 956 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 159 GAGTC GAGTC(4/5) RcaI 536 TCATGA T^CATGA SacI 286 GAGCTC GAGCT^C Sau3AI 513 GATC ^GATC SexAI 800 ACCTGGT A^CC[AT]GGT SfoI 530 GGCGCC GGC^GCC SspBI 806 TGTACA T^GTACA TaqI 479 TCGA T^CGA TfiI 120 GATTC G^A[AT]TC Tsp509I 980 AATT ^AATT TthHB8I 479 TCGA T^CGA Van91I 956 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 573 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 49 GGCGTC G[GA]^CG[CT]C 530 GGCGCC AspHI 71 GAGCAC G[AT]GC[AT]^C 286 GAGCTC AvaII 579 GGACC G^G[AT]CC 644 GGACC AviII 755 TGCGCA TGC^GCA 770 TGCGCA BcnI 488 CCCGG CC^[GC]GG 895 CCCGG BfaI 130 CTAG C^TAG 574 CTAG BsaHI 49 GGCGTC G[GA]^CG[CT]C 530 GGCGCC BsgI 624 CTGCAC GTGCAG(16/14) 706 GTGCAG BsiHKAI 71 GAGCAC G[AT]GC[AT]^C 286 GAGCTC BsmAI 793 GAGAC GTCTC(1/5) 875 GTCTC BstF5I 80 CATCC GGATG(2/0) 584 GGATG DraIII 607 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG 915 CACCATGTG FauI 135 CCCGC CCCGC(4/6) 723 GCGGG FokI 80 CATCC GGATG(9/13) 584 GGATG FspI 755 TGCGCA TGC^GCA 770 TGCGCA HaeII 491 GGCGCT [GA]GCGC^[CT] 530 GGCGCC Hin1I 49 GGCGTC G[GA]^CG[CT]C 530 GGCGCC HinfI 120 GATTC G^A[ACGT]TC 159 GAGTC KspI 136 CCGCGG CCGC^GG 332 CCGCGG MaeI 130 CTAG C^TAG 574 CTAG NciI 488 CCCGG CC^[GC]GG 895 CCCGG PstI 366 CTGCAG CTGCA^G 1014 CTGCAG PvuII 589 CAGCTG CAG^CTG 766 CAGCTG SacII 136 CCGCGG CCGC^GG 332 CCGCGG SfcI 366 CTGCAG C^T[GA][CT]AG 1014 CTGCAG Tsp45I 355 GTCAC ^GT[GC]AC 911 GTGAC XcmI 606 CCACGGCGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 848 CCACGGGCAGCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AfaI AluI BbsI BbvI BmyI BpuAI BsaAI BsaJI BsiEI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TseI TspRI