Restriction map for
pFN21AE4470
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI AlwNI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BanII BbeI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRI EcoT22I FseI FspI HaeII
Hin1I HpaI KasI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 370 GGTACC G^GTACC
AccI 106 GTCTAC GT^[AC][GT]AC
AccII 783 CGCG CG^CG
AflIII 836 ACGTGT A^C[GA][CT]GT
Alw44I 228 GTGCAC G^TGCAC
AocI 365 CCTCAGG CC^T[ACGT]AGG
ApaLI 228 GTGCAC G^TGCAC
Asp718I 370 GGTACC G^GTACC
BamHI 613 GGATCC G^GATCC
BbrPI 835 CACGTG CAC^GTG
BbsI 1173 GAAGAC GAAGAC(2/6)
BclI 1256 TGATCA T^GATCA
BpuAI 1173 GAAGAC GAAGAC(2/6)
BsaAI 835 CACGTG [CT]AC^GT[GA]
BsaWI 63 ACCGGA [AT]^CCGG[AT]
BssHII 782 GCGCGC G^CGCGC
BssSI 283 CTCGTG CACGAG(-5/-1)
BstAPI 329 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1341 TTCGAA TT^CGAA
BstEII 17 GGTCACC G^GT[ACGT]ACC
BstPI 17 GGTCACC G^GT[ACGT]ACC
BstUI 783 CGCG CG^CG
BstXI 913 CCATGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 613 GGATCC [GA]^GATC[CT]
Bsu36I 365 CCTCAGG CC^T[ACGT]AGG
EaeI 299 TGGCCG [CT]^GGCC[GA]
Eco81I 365 CCTCAGG CC^T[ACGT]AGG
EcoNI 168 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 17 GGTCACC G^GT[ACGT]ACC
EcoRV 658 GATATC GAT^ATC
FbaI 1256 TGATCA T^GATCA
HgaI 171 GACGC GACGC(5/10)
HincII 736 GTCAAC GT[CT]^[GA]AC
HindII 736 GTCAAC GT[CT]^[GA]AC
HindIII 1400 AAGCTT A^AGCTT
KpnI 370 GGTACC GGTAC^C
MflI 613 GGATCC [GA]^GATC[CT]
MseI 905 TTAA T^TAA
MvnI 783 CGCG CG^CG
NspV 1341 TTCGAA TT^CGAA
PmaCI 835 CACGTG CAC^GTG
PmlI 835 CACGTG CAC^GTG
ScaI 128 AGTACT AGT^ACT
SfuI 1341 TTCGAA TT^CGAA
SmaI 789 CCCGGG CCC^GGG
SmlI 747 CTCAAG C^T[CT][GA]AG
Tru9I 905 TTAA T^TAA
XcmI 1311 CCAGGGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 613 GGATCC [GA]^GATC[CT]
XmaCI 789 CCCGGG C^CCGGG
XmaI 789 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 228 GTGCAC G[AT]GC[AT]^C
336 GTGCTC
BanI 370 GGTACC G^G[CT][GA]CC
503 GGCACC
BfaI 587 CTAG C^TAG
1078 CTAG
BseRI 363 CTCCTC GAGGAG(10/8)
1162 GAGGAG
BsgI 195 CTGCAC GTGCAG(16/14)
817 CTGCAC
BsiHKAI 228 GTGCAC G[AT]GC[AT]^C
336 GTGCTC
BsmAI 945 GAGAC GTCTC(1/5)
1216 GAGAC
BspMI 373 ACCTGC ACCTGC(4/8)
807 GCAGGT
BsrDI 1035 CATTGC GCAATG(2/0)
650 GCAATG
BsrFI 646 GCCGGC [GA]^CCGG[CT]
1279 GCCGGC
Cfr10I 646 GCCGGC [GA]^CCGG[CT]
1279 GCCGGC
DraIII 505 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
886 CACTGCGTG
DsaI 51 CCATGG C^C[GA][CT]GG
913 CCATGG
MaeI 587 CTAG C^TAG
1078 CTAG
MslI 679 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1220 CACAGAGATG
MspA1I 302 CCGCTG C[AC]G^C[GT]G
609 CAGCGG
NaeI 646 GCCGGC GCC^GGC
1279 GCCGGC
NcoI 51 CCATGG C^CATGG
913 CCATGG
NgoMIV 646 GCCGGC G^CCGGC
1279 GCCGGC
PflMI 25 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
885 CCACTGCGTGG
PleI 361 GACTC GAGTC(4/5)
1012 GACTC
PpuMI 164 GGGTCCT [GA]G^G[AT]CC[CT]
274 GGGACCT
SbfI 551 CCTGCAGG CCTGCA^GG
1287 CCTGCAGG
Sse8387I 551 CCTGCAGG CCTGCA^GG
1287 CCTGCAGG
TfiI 435 GAATC G^A[AT]TC
1303 GATTC
Tsp509I 577 AATT ^AATT
1006 AATT
Van91I 25 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
885 CCACTGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BbvI BciVI BcnI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I