Restriction map for
pFN21AE5138
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TfiI Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 71 GTAC GT^AC
AlwI 149 GGATC GGATC(4/5)
AlwNI 47 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 135 AGCGCT AGC^GCT
AspHI 238 GAGCAC G[AT]GC[AT]^C
AvaI 248 CTCGAG C^[CT]CG[GA]G
BcgI 240 GCACTTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 238 GAGCAC G[AGT]GC[ACT]^C
BseRI 95 GAGGAG GAGGAG(10/8)
BsiHKAI 238 GAGCAC G[AT]GC[AT]^C
BsiWI 70 CGTACG C^GTACG
BsiYI 35 CCATGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 35 CCATGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 248 CTCGAG C^[CT]CG[GA]G
Bsp1286I 238 GAGCAC G[AGT]GC[ACT]^C
BsrI 328 CCAGT ACTGG(1/-1)
BstYI 149 GGATCT [GA]^GATC[CT]
Cac8I 15 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 136 GCGC GCG^C
Cfr13I 81 GGCCC G^G[ACGT]CC
DpnII 150 GATC ^GATC
DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 43 CGGCCA [CT]^GGCC[GA]
EarI 363 GAAGAG CTCTTC(1/4)
Eco47III 135 AGCGCT AGC^GCT
EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 135 AGCGCT [GA]GCGC^[CT]
HhaI 136 GCGC GCG^C
HinP1I 136 GCGC G^CGC
HphI 127 GGTGA GGTGA(8/7)
Ksp632I 363 GAAGAG CTCTTC(1/4)
MaeII 69 ACGT A^CGT
MboI 150 GATC ^GATC
MflI 149 GGATCT [GA]^GATC[CT]
MseI 22 TTAA T^TAA
MwoI 208 GCACCCACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 150 GATC ^GATC
PaeR7I 248 CTCGAG C^TCGAG
PvuII 218 CAGCTG CAG^CTG
RsaI 71 GTAC GT^AC
Sau3AI 150 GATC ^GATC
Sau96I 81 GGCCC G^G[ACGT]CC
SmlI 248 CTCGAG C^T[CT][GA]AG
SspI 274 AATATT AAT^ATT
TaqI 249 TCGA T^CGA
Tru9I 22 TTAA T^TAA
Tsp509I 347 AATT ^AATT
TthHB8I 249 TCGA T^CGA
XhoI 248 CTCGAG C^TCGAG
XhoII 149 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AluI 14 AGCT AG^CT
219 AGCT
BsaJI 123 CCTGGG C^C[ACGT][ACGT]GG
296 CCCAGG
BsmAI 49 GAGAC GTCTC(1/5)
246 GTCTC
BssKI 123 CCTGG ^CC[ACGT]GG
297 CCAGG
BstNI 123 CCTGG CC^[AT]GG
297 CCAGG
EcoRII 123 CCTGG ^CC[AT]GG
297 CCAGG
FauI 5 CCCGC CCCGC(4/6)
182 GCGGG
HaeIII 44 GGCC GG^CC
81 GGCC
MboII 363 GAAGA GAAGA(8/7)
369 GAAGA
MspA1I 40 CAGCGG C[AC]G^C[GT]G
218 CAGCTG
MvaI 123 CCTGG CC^[AT]GG
297 CCAGG
NlaIII 36 CATG CATG^
231 CATG
ScrFI 123 CCTGG CC^[ACGT]GG
297 CCAGG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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AciI BbvI BsmFI Fnu4HI ItaI
MnlI TseI