Restriction map for
pFN21AE2699
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I AspEI AspI AviII BbvCI
BciVI BglII BlpI Bpu1102I BsaI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI Ksp632I KspI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1684 GGTACC G^GTACC
AccI 554 GTCTAC GT^[AC][GT]AC
AcyI 1061 GGCGCC G[GA]^CG[CT]C
AflII 718 CTTAAG C^TTAAG
AflIII 866 ACGTGT A^C[GA][CT]GT
ApaI 1514 GGGCCC GGGCC^C
AseI 2703 ATTAAT AT^TAAT
AsnI 2703 ATTAAT AT^TAAT
Asp718I 1684 GGTACC G^GTACC
AvrII 1650 CCTAGG C^CTAGG
BamHI 2216 GGATCC G^GATCC
BbeI 1061 GGCGCC GGCGC^C
BbrPI 865 CACGTG CAC^GTG
BclI 1088 TGATCA T^GATCA
BfrI 718 CTTAAG C^TTAAG
BglI 2002 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1650 CCTAGG C^CTAGG
BsaBI 1159 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1061 GGCGCC G[GA]^CG[CT]C
BsaWI 1670 ACCGGA [AT]^CCGG[AT]
BsgI 355 GTGCAG GTGCAG(16/14)
BsmFI 44 GGGAC GGGAC(10/14)
BsmI 1661 GCATTC GAATGC(1/-1)
BstBI 2726 TTCGAA TT^CGAA
FbaI 1088 TGATCA T^GATCA
HaeII 1061 GGCGCC [GA]GCGC^[CT]
HgaI 2280 GACGC GACGC(5/10)
Hin1I 1061 GGCGCC G[GA]^CG[CT]C
KasI 1061 GGCGCC G^GCGCC
KpnI 1684 GGTACC GGTAC^C
MamI 1159 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1061 GGCGCC GG^CGCC
NdeI 2419 CATATG CA^TATG
NspI 286 GCATGT [GA]CATG^[CT]
NspV 2726 TTCGAA TT^CGAA
PmaCI 865 CACGTG CAC^GTG
PmlI 865 CACGTG CAC^GTG
PshBI 2703 ATTAAT AT^TAAT
SfoI 1061 GGCGCC GGC^GCC
SfuI 2726 TTCGAA TT^CGAA
SmaI 28 CCCGGG CCC^GGG
SpeI 1491 ACTAGT A^CTAGT
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 278 CGCG CG^CG
2244 CGCG
AcsI 1263 GAATTC [GA]^AATT[CT]
2566 GAATTC
AlwNI 704 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
724 CAGATTCTG
ApoI 1263 GAATTC [GA]^AATT[CT]
2566 GAATTC
BalI 992 TGGCCA TGG^CCA
1331 TGGCCA
BanI 1061 GGCGCC G^G[CT][GA]CC
1684 GGTACC
BbsI 532 GAAGAC GAAGAC(2/6)
2464 GAAGAC
BcgI 11 GCACACTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
368 GCAATGCTCTCG
BpmI 579 CTCCAG CTGGAG(16/14)
1724 CTCCAG
BpuAI 532 GAAGAC GAAGAC(2/6)
2464 GAAGAC
BsaAI 865 CACGTG [CT]AC^GT[GA]
1606 TACGTG
Bsp1407I 983 TGTACA T^GTACA
1382 TGTACA
BspHI 1643 TCATGA T^CATGA
2168 TCATGA
BsrGI 983 TGTACA T^GTACA
1382 TGTACA
BstUI 278 CGCG CG^CG
2244 CGCG
CfoI 1062 GCGC GCG^C
2245 GCGC
DsaI 893 CCATGG C^C[GA][CT]GG
1057 CCATGG
EcoRI 1263 GAATTC G^AATTC
2566 GAATTC
HhaI 1062 GCGC GCG^C
2245 GCGC
HinP1I 1062 GCGC G^CGC
2245 GCGC
MluNI 992 TGGCCA TGG^CCA
1331 TGGCCA
MscI 992 TGGCCA TGG^CCA
1331 TGGCCA
MslI 735 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1602 CACCTACGTG
MvnI 278 CGCG CG^CG
2244 CGCG
NcoI 893 CCATGG C^CATGG
1057 CCATGG
PstI 904 CTGCAG CTGCA^G
1036 CTGCAG
RcaI 1643 TCATGA T^CATGA
2168 TCATGA
SacI 562 GAGCTC GAGCT^C
1895 GAGCTC
ScaI 105 AGTACT AGT^ACT
752 AGTACT
SspBI 983 TGTACA T^GTACA
1382 TGTACA
SspI 589 AATATT AAT^ATT
2500 AATATT
XcmI 294 CCAGCTGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
609 CCAGCTGCCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII