Restriction map for
pFN21AE1708
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AlwI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AvaII AviII AvrII
BalI BamHI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FbaI FokI FseI FspI HapII
HincII HindII HindIII HpaI HpaII
KpnI Ksp632I MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 542 AAATTT [GA]^AATT[CT]
AcyI 49 GGCGCC G[GA]^CG[CT]C
AhdI 561 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 222 GTGCAC G^TGCAC
ApaLI 222 GTGCAC G^TGCAC
ApoI 542 AAATTT [GA]^AATT[CT]
AspEI 561 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 222 GTGCAC G[AT]GC[AT]^C
BanI 49 GGCGCC G^G[CT][GA]CC
BanII 70 GAGCCC G[GA]GC[CT]^C
BbeI 49 GGCGCC GGCGC^C
BbrPI 37 CACGTG CAC^GTG
BbsI 558 GAAGAC GAAGAC(2/6)
BglI 121 GCCGAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 480 AGATCT A^GATCT
BpmI 106 CTGGAG CTGGAG(16/14)
BpuAI 558 GAAGAC GAAGAC(2/6)
BsaAI 37 CACGTG [CT]AC^GT[GA]
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsgI 584 CTGCAC GTGCAG(16/14)
BsiHKAI 222 GTGCAC G[AT]GC[AT]^C
BsiYI 25 CCGTTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 25 CCGTTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 262 GAGAC GTCTC(1/5)
BsmI 181 GCATTC GAATGC(1/-1)
Bsp1407I 215 TGTACA T^GTACA
BspLU11I 218 ACATGT A^CATGT
BsrGI 215 TGTACA T^GTACA
BstAPI 112 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 480 AGATCT [GA]^GATC[CT]
DraII 201 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 561 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 201 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 229 GATATC GAT^ATC
FauI 73 CCCGC CCCGC(4/6)
HaeII 49 GGCGCC [GA]GCGC^[CT]
HgaI 100 GACGC GACGC(5/10)
Hin1I 49 GGCGCC G[GA]^CG[CT]C
HinfI 381 GACTC G^A[ACGT]TC
KasI 49 GGCGCC G^GCGCC
MflI 480 AGATCT [GA]^GATC[CT]
NarI 49 GGCGCC GG^CGCC
NspI 218 ACATGT [GA]CATG^[CT]
PleI 381 GACTC GAGTC(4/5)
PmaCI 37 CACGTG CAC^GTG
PmlI 37 CACGTG CAC^GTG
SfoI 49 GGCGCC GGC^GCC
SspBI 215 TGTACA T^GTACA
Tsp509I 543 AATT ^AATT
XhoII 480 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
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AluI 249 AGCT AG^CT
420 AGCT
AlwNI 113 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
161 CAGGGTCTG
AocI 205 CCTCAGG CC^T[ACGT]AGG
238 CCTGAGG
BfaI 187 CTAG C^TAG
259 CTAG
BsrI 126 ACTGG ACTGG(1/-1)
295 CCAGT
BssKI 147 CCTGG ^CC[ACGT]GG
471 CCTGG
BstNI 147 CCTGG CC^[AT]GG
471 CCTGG
Bsu36I 205 CCTCAGG CC^T[ACGT]AGG
238 CCTGAGG
CfoI 50 GCGC GCG^C
103 GCGC
Cfr13I 42 GGGCC G^G[ACGT]CC
202 GGCCC
DdeI 206 CTCAG C^T[ACGT]AG
239 CTGAG
DpnII 349 GATC ^GATC
481 GATC
Eco81I 205 CCTCAGG CC^T[ACGT]AGG
238 CCTGAGG
EcoRII 147 CCTGG ^CC[AT]GG
471 CCTGG
HhaI 50 GCGC GCG^C
103 GCGC
HinP1I 50 GCGC G^CGC
103 GCGC
HphI 490 TCACC GGTGA(8/7)
632 TCACC
KspI 45 CCGCGG CCGC^GG
74 CCGCGG
MaeI 187 CTAG C^TAG
259 CTAG
MboI 349 GATC ^GATC
481 GATC
MseI 362 TTAA T^TAA
521 TTAA
MvaI 147 CCTGG CC^[AT]GG
471 CCTGG
NdeII 349 GATC ^GATC
481 GATC
NlaIII 219 CATG CATG^
453 CATG
SacII 45 CCGCGG CCGC^GG
74 CCGCGG
Sau3AI 349 GATC ^GATC
481 GATC
Sau96I 42 GGGCC G^G[ACGT]CC
202 GGCCC
ScrFI 147 CCTGG CC^[ACGT]GG
471 CCTGG
SmlI 389 CTCAAG C^T[CT][GA]AG
625 CTCAAG
Tru9I 362 TTAA T^TAA
521 TTAA
Tsp45I 379 GTGAC ^GT[GC]AC
404 GTCAC
TspRI 225 CACTG CA[GC]TG[ACGT][ACGT]^
582 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AccII AciI AfaI AflIII BbvI
BmyI BsaJI Bsp1286I BstUI Cac8I
DsaI Fnu4HI HaeIII ItaI MaeII
MaeIII MboII MnlI MspA1I MvnI
MwoI NlaIV RsaI TseI