Restriction map for
pFN21AB9686
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BamHI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpmI
BpuAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MamI MboII
MfeI MflI MroI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NlaIII NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AfaI 42 GTAC GT^AC
AflIII 95 ACGCGT A^C[GA][CT]GT
AviII 284 TGCGCA TGC^GCA
BalI 371 TGGCCA TGG^CCA
BanI 445 GGCGCC G^G[CT][GA]CC
BbeI 445 GGCGCC GGCGC^C
BbrPI 34 CACGTG CAC^GTG
BbvCI 504 CCTCAGC CCTCAGC(-5/-2)
BlpI 60 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC
BsaAI 34 CACGTG [CT]AC^GT[GA]
BseRI 73 GAGGAG GAGGAG(10/8)
BsgI 492 CTGCAC GTGCAG(16/14)
BsmAI 334 GAGAC GTCTC(1/5)
BsmBI 334 GAGACG CGTCTC(1/5)
BsrBI 241 GAGCGG CCGCTC(-3/-3)
BsrFI 306 GCCGGT [GA]^CCGG[CT]
BsrI 105 CCAGT ACTGG(1/-1)
BssHII 29 GCGCGC G^CGCGC
BstAPI 494 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 294 CATCC GGATG(2/0)
CelII 60 GCTGAGC GC^T[ACGT]AGC
Cfr10I 306 GCCGGT [GA]^CCGG[CT]
DpnII 330 GATC ^GATC
EcoT14I 440 CCAAGG C^C[AT][AT]GG
FokI 294 CATCC GGATG(9/13)
FspI 284 TGCGCA TGC^GCA
HinfI 394 GACTC G^A[ACGT]TC
HphI 561 GGTGA GGTGA(8/7)
KasI 445 GGCGCC G^GCGCC
KspI 428 CCGCGG CCGC^GG
MaeIII 536 GTGAC ^GT[ACGT]AC
MboI 330 GATC ^GATC
MluI 95 ACGCGT A^CGCGT
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
NarI 445 GGCGCC GG^CGCC
NdeII 330 GATC ^GATC
NotI 470 GCGGCCGC GC^GGCCGC
PleI 394 GACTC GAGTC(4/5)
PmaCI 34 CACGTG CAC^GTG
PmlI 34 CACGTG CAC^GTG
PvuII 549 CAGCTG CAG^CTG
RsaI 42 GTAC GT^AC
SacII 428 CCGCGG CCGC^GG
Sau3AI 330 GATC ^GATC
SfoI 445 GGCGCC GGC^GCC
StyI 440 CCAAGG C^C[AT][AT]GG
Tsp45I 536 GTGAC ^GT[GC]AC
TspRI 435 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 299 CCACCGAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 348 GGCGTC G[GA]^CG[CT]C
445 GGCGCC
AvaI 150 CCCGGG C^[CT]CG[GA]G
157 CCCGGG
BglI 155 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
271 GCCTTCACGGC
BsaHI 348 GGCGTC G[GA]^CG[CT]C
445 GGCGCC
BsiEI 398 CGGCCG CG[GA][CT]^CG
471 CGGCCG
BsmFI 245 GGGAC GGGAC(10/14)
451 GTCCC
BsoBI 150 CCCGGG C^[CT]CG[GA]G
157 CCCGGG
EagI 398 CGGCCG C^GGCCG
471 CGGCCG
EclXI 398 CGGCCG C^GGCCG
471 CGGCCG
Eco52I 398 CGGCCG C^GGCCG
471 CGGCCG
HaeII 279 GGCGCT [GA]GCGC^[CT]
445 GGCGCC
Hin1I 348 GGCGTC G[GA]^CG[CT]C
445 GGCGCC
SmaI 150 CCCGGG CCC^GGG
157 CCCGGG
TaqI 332 TCGA T^CGA
582 TCGA
TthHB8I 332 TCGA T^CGA
582 TCGA
XmaCI 150 CCCGGG C^CCGGG
157 CCCGGG
XmaI 150 CCCGGG C^CCGGG
157 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI