################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpmI BpuAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MboII MfeI MflI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 42 GTAC GT^AC AflIII 95 ACGCGT A^C[GA][CT]GT AviII 284 TGCGCA TGC^GCA BalI 371 TGGCCA TGG^CCA BanI 445 GGCGCC G^G[CT][GA]CC BbeI 445 GGCGCC GGCGC^C BbrPI 34 CACGTG CAC^GTG BbvCI 504 CCTCAGC CCTCAGC(-5/-2) BlpI 60 GCTGAGC GC^T[ACGT]AGC Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC BsaAI 34 CACGTG [CT]AC^GT[GA] BseRI 73 GAGGAG GAGGAG(10/8) BsgI 492 CTGCAC GTGCAG(16/14) BsmAI 334 GAGAC GTCTC(1/5) BsmBI 334 GAGACG CGTCTC(1/5) BsrBI 241 GAGCGG CCGCTC(-3/-3) BsrFI 306 GCCGGT [GA]^CCGG[CT] BsrI 105 CCAGT ACTGG(1/-1) BssHII 29 GCGCGC G^CGCGC BstAPI 494 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 294 CATCC GGATG(2/0) CelII 60 GCTGAGC GC^T[ACGT]AGC Cfr10I 306 GCCGGT [GA]^CCGG[CT] DpnII 330 GATC ^GATC EcoT14I 440 CCAAGG C^C[AT][AT]GG FokI 294 CATCC GGATG(9/13) FspI 284 TGCGCA TGC^GCA HinfI 394 GACTC G^A[ACGT]TC HphI 561 GGTGA GGTGA(8/7) KasI 445 GGCGCC G^GCGCC KspI 428 CCGCGG CCGC^GG MaeIII 536 GTGAC ^GT[ACGT]AC MboI 330 GATC ^GATC MluI 95 ACGCGT A^CGCGT MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA NarI 445 GGCGCC GG^CGCC NdeII 330 GATC ^GATC NotI 470 GCGGCCGC GC^GGCCGC PleI 394 GACTC GAGTC(4/5) PmaCI 34 CACGTG CAC^GTG PmlI 34 CACGTG CAC^GTG PvuII 549 CAGCTG CAG^CTG RsaI 42 GTAC GT^AC SacII 428 CCGCGG CCGC^GG Sau3AI 330 GATC ^GATC SfoI 445 GGCGCC GGC^GCC StyI 440 CCAAGG C^C[AT][AT]GG Tsp45I 536 GTGAC ^GT[GC]AC TspRI 435 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 299 CCACCGAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 348 GGCGTC G[GA]^CG[CT]C 445 GGCGCC AvaI 150 CCCGGG C^[CT]CG[GA]G 157 CCCGGG BglI 155 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 271 GCCTTCACGGC BsaHI 348 GGCGTC G[GA]^CG[CT]C 445 GGCGCC BsiEI 398 CGGCCG CG[GA][CT]^CG 471 CGGCCG BsmFI 245 GGGAC GGGAC(10/14) 451 GTCCC BsoBI 150 CCCGGG C^[CT]CG[GA]G 157 CCCGGG EagI 398 CGGCCG C^GGCCG 471 CGGCCG EclXI 398 CGGCCG C^GGCCG 471 CGGCCG Eco52I 398 CGGCCG C^GGCCG 471 CGGCCG HaeII 279 GGCGCT [GA]GCGC^[CT] 445 GGCGCC Hin1I 348 GGCGTC G[GA]^CG[CT]C 445 GGCGCC SmaI 150 CCCGGG CCC^GGG 157 CCCGGG TaqI 332 TCGA T^CGA 582 TCGA TthHB8I 332 TCGA T^CGA 582 TCGA XmaCI 150 CCCGGG C^CCGGG 157 CCCGGG XmaI 150 CCCGGG C^CCGGG 157 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AluI ApaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI