Restriction map for
pFN21AE5074
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HindIII HpaI KpnI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI Sau3AI ScaI
SexAI SfaNI SfuI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 301 GTCTAC GT^[AC][GT]AC
AlwNI 11 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 403 AGCGCT AGC^GCT
ApaI 516 GGGCCC GGGCC^C
Asp700I 381 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 252 CACGTG CAC^GTG
BfaI 298 CTAG C^TAG
BsaAI 252 CACGTG [CT]AC^GT[GA]
BsaI 440 GGTCTC GGTCTC(1/5)
BsgI 339 CTGCAC GTGCAG(16/14)
BsmBI 150 CGTCTC CGTCTC(1/5)
BsmFI 3 GGGAC GGGAC(10/14)
BspMI 230 ACCTGC ACCTGC(4/8)
BsrBI 324 CCGCTC CCGCTC(-3/-3)
BsrI 477 CCAGT ACTGG(1/-1)
DraIII 135 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 403 AGCGCT AGC^GCT
EcoNI 375 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 263 CCATGG C^C[AT][AT]GG
HincII 145 GTCAAC GT[CT]^[GA]AC
HindII 145 GTCAAC GT[CT]^[GA]AC
HinfI 131 GAGTC G^A[ACGT]TC
HphI 396 GGTGA GGTGA(8/7)
MaeI 298 CTAG C^TAG
MaeIII 133 GTCAC ^GT[ACGT]AC
NaeI 283 GCCGGC GCC^GGC
NcoI 263 CCATGG C^CATGG
NgoMIV 283 GCCGGC G^CCGGC
PleI 131 GAGTC GAGTC(4/5)
PmaCI 252 CACGTG CAC^GTG
PmlI 252 CACGTG CAC^GTG
PpuMI 227 AGGACCT [GA]G^G[AT]CC[CT]
PstI 487 CTGCAG CTGCA^G
PvuII 115 CAGCTG CAG^CTG
SapI 399 GAAGAGC GCTCTTC(1/4)
SbfI 486 CCTGCAGG CCTGCA^GG
SfcI 487 CTGCAG C^T[GA][CT]AG
SfiI 465 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 282 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 429 CCCGGG CCC^GGG
Sse8387I 486 CCTGCAGG CCTGCA^GG
StuI 383 AGGCCT AGG^CCT
StyI 263 CCATGG C^C[AT][AT]GG
TaqI 212 TCGA T^CGA
Tsp45I 133 GTCAC ^GT[GC]AC
TthHB8I 212 TCGA T^CGA
XmaCI 429 CCCGGG C^CCGGG
XmaI 429 CCCGGG C^CCGGG
XmnI 381 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 20 GGCGCC G[GA]^CG[CT]C
289 GGCGCC
AfaI 43 GTAC GT^AC
348 GTAC
AocI 506 CCTTAGG CC^T[ACGT]AGG
520 CCTGAGG
AvaI 82 CTCGGG C^[CT]CG[GA]G
429 CCCGGG
AvaII 4 GGACC G^G[AT]CC
228 GGACC
BbeI 20 GGCGCC GGCGC^C
289 GGCGCC
BbvCI 369 CCTCAGC CCTCAGC(-5/-2)
223 GCTGAGG
BglI 262 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
466 GCCCTCCAGGC
BsaHI 20 GGCGCC G[GA]^CG[CT]C
289 GGCGCC
BseRI 109 GAGGAG GAGGAG(10/8)
460 GAGGAG
BsiEI 315 CGGCCG CG[GA][CT]^CG
329 CGGCCG
BsmAI 151 GTCTC GTCTC(1/5)
441 GTCTC
BsoBI 82 CTCGGG C^[CT]CG[GA]G
429 CCCGGG
BsrFI 6 ACCGGC [GA]^CCGG[CT]
283 GCCGGC
Bsu36I 506 CCTTAGG CC^T[ACGT]AGG
520 CCTGAGG
Cfr10I 6 ACCGGC [GA]^CCGG[CT]
283 GCCGGC
EaeI 315 CGGCCG [CT]^GGCC[GA]
329 CGGCCG
EagI 315 CGGCCG C^GGCCG
329 CGGCCG
EarI 76 CTCTTC CTCTTC(1/4)
399 GAAGAG
EclXI 315 CGGCCG C^GGCCG
329 CGGCCG
Eco52I 315 CGGCCG C^GGCCG
329 CGGCCG
Eco81I 506 CCTTAGG CC^T[ACGT]AGG
520 CCTGAGG
HgaI 62 GCGTC GACGC(5/10)
143 GCGTC
Hin1I 20 GGCGCC G[GA]^CG[CT]C
289 GGCGCC
KasI 20 GGCGCC G^GCGCC
289 GGCGCC
Ksp632I 76 CTCTTC CTCTTC(1/4)
399 GAAGAG
MaeII 149 ACGT A^CGT
253 ACGT
MboII 399 GAAGA GAAGA(8/7)
77 TCTTC
NarI 20 GGCGCC GG^CGCC
289 GGCGCC
NlaIII 264 CATG CATG^
354 CATG
RsaI 43 GTAC GT^AC
348 GTAC
SfoI 20 GGCGCC GGC^GCC
289 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI KspI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV SacII Sau96I
ScrFI TseI