Restriction map for
pFN21AE2054
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BfrI BglI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 21 enzymes) #
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AcyI 163 GACGCC G[GA]^CG[CT]C
AfaI 530 GTAC GT^AC
BanI 1122 GGTGCC G^G[CT][GA]CC
BbvCI 1345 CCTCAGC CCTCAGC(-5/-2)
BsaHI 163 GACGCC G[GA]^CG[CT]C
BsgI 103 CTGCAC GTGCAG(16/14)
BsiEI 807 CGACCG CG[GA][CT]^CG
BspHI 143 TCATGA T^CATGA
BsrBI 712 GAGCGG CCGCTC(-3/-3)
DraI 961 TTTAAA TTT^AAA
DsaI 1727 CCGTGG C^C[GA][CT]GG
EcoRI 1172 GAATTC G^AATTC
Hin1I 163 GACGCC G[GA]^CG[CT]C
PflMI 1836 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 866 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1765 CAGCTG CAG^CTG
RcaI 143 TCATGA T^CATGA
RsaI 530 GTAC GT^AC
SacI 1747 GAGCTC GAGCT^C
Van91I 1836 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1046 CCAAAAATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 43 enzymes) #
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AlwI 1043 GATCC GGATC(4/5)
1712 GGATC
AlwNI 983 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1573 CAGACCCTG
AspHI 856 GAGCAC G[AT]GC[AT]^C
1747 GAGCTC
AvaI 665 CCCGGG C^[CT]CG[GA]G
1208 CCCGGG
BalI 266 TGGCCA TGG^CCA
1104 TGGCCA
BanII 1732 GAGCCC G[GA]GC[CT]^C
1747 GAGCTC
BbsI 568 GAAGAC GAAGAC(2/6)
1627 GAAGAC
BclI 1320 TGATCA T^GATCA
1769 TGATCA
BglII 433 AGATCT A^GATCT
1790 AGATCT
BlpI 78 GCTGAGC GC^T[ACGT]AGC
1744 GCTGAGC
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
1744 GCTGAGC
BpuAI 568 GAAGAC GAAGAC(2/6)
1627 GAAGAC
BseRI 503 GAGGAG GAGGAG(10/8)
1402 GAGGAG
BsiHKAI 856 GAGCAC G[AT]GC[AT]^C
1747 GAGCTC
BsmBI 506 GAGACG CGTCTC(1/5)
1438 GAGACG
BsoBI 665 CCCGGG C^[CT]CG[GA]G
1208 CCCGGG
BspMI 101 ACCTGC ACCTGC(4/8)
1098 ACCTGC
BsrI 136 ACTGG ACTGG(1/-1)
1073 CCAGT
BstXI 227 CCATAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
324 CCAGATAATTGG
BstYI 433 AGATCT [GA]^GATC[CT]
1790 AGATCT
CelII 78 GCTGAGC GC^T[ACGT]AGC
1744 GCTGAGC
EaeI 266 TGGCCA [CT]^GGCC[GA]
1104 TGGCCA
EcoNI 216 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1695 CCTGCAGCAGG
FauI 289 GCGGG CCCGC(4/6)
714 GCGGG
FbaI 1320 TGATCA T^GATCA
1769 TGATCA
HaeII 33 GGCGCT [GA]GCGC^[CT]
1236 GGCGCT
MflI 433 AGATCT [GA]^GATC[CT]
1790 AGATCT
MluNI 266 TGGCCA TGG^CCA
1104 TGGCCA
MscI 266 TGGCCA TGG^CCA
1104 TGGCCA
MseI 962 TTAA T^TAA
1738 TTAA
MslI 117 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
228 CATAGACGTG
MspA1I 61 CCGCTG C[AC]G^C[GT]G
1765 CAGCTG
PstI 1654 CTGCAG CTGCA^G
1696 CTGCAG
SfcI 1654 CTGCAG C^T[GA][CT]AG
1696 CTGCAG
SmaI 665 CCCGGG CCC^GGG
1208 CCCGGG
SmlI 429 CTCAAG C^T[CT][GA]AG
1816 CTCAAG
TaqI 543 TCGA T^CGA
1867 TCGA
TfiI 48 GATTC G^A[AT]TC
281 GAATC
Tru9I 962 TTAA T^TAA
1738 TTAA
TthHB8I 543 TCGA T^CGA
1867 TCGA
XhoII 433 AGATCT [GA]^GATC[CT]
1790 AGATCT
XmaCI 665 CCCGGG C^CCGGG
1208 CCCGGG
XmaI 665 CCCGGG C^CCGGG
1208 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
Tsp509I TspRI