Restriction map for
pFN21AE9254
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BbeI BbrPI BbvCI
BlpI Bpu1102I BsiEI BsiWI BsmI
BsoBI BspDI BspHI BsrBI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FseI FspI HaeII HpaI
KasI KpnI MluI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 2071 TCCGGA T^CCGGA
AclI 858 AACGTT AA^CGTT
AflII 732 CTTAAG C^TTAAG
Asp700I 1637 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1509 CCTAGG C^CTAGG
BalI 155 TGGCCA TGG^CCA
BamHI 645 GGATCC G^GATCC
BcgI 1170 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 574 GGATAC GTATCC(6/5)
BclI 1314 TGATCA T^GATCA
BfrI 732 CTTAAG C^TTAAG
BglI 1427 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1519 AGATCT A^GATCT
BlnI 1509 CCTAGG C^CTAGG
BsaAI 524 TACGTG [CT]AC^GT[GA]
BsaBI 1299 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2071 TCCGGA [AT]^CCGG[AT]
BseAI 2071 TCCGGA T^CCGGA
BsmBI 172 GAGACG CGTCTC(1/5)
Bsp1407I 1397 TGTACA T^GTACA
BspEI 2071 TCCGGA T^CCGGA
BsrDI 767 GCAATG GCAATG(2/0)
BsrFI 815 GCCGGC [GA]^CCGG[CT]
BsrGI 1397 TGTACA T^GTACA
BssHII 15 GCGCGC G^CGCGC
BstEII 607 GGTGACC G^GT[ACGT]ACC
BstPI 607 GGTGACC G^GT[ACGT]ACC
BstXI 665 CCACAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 815 GCCGGC [GA]^CCGG[CT]
DraI 1056 TTTAAA TTT^AAA
DrdI 2113 GACAACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 155 TGGCCA [CT]^GGCC[GA]
EcoNI 988 CCTGTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 607 GGTGACC G^GT[ACGT]ACC
EcoRV 1850 GATATC GAT^ATC
FbaI 1314 TGATCA T^GATCA
HincII 1084 GTCAAC GT[CT]^[GA]AC
HindII 1084 GTCAAC GT[CT]^[GA]AC
KspI 257 CCGCGG CCGC^GG
MamI 1299 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1964 CAATTG C^AATTG
MluNI 155 TGGCCA TGG^CCA
MroI 2071 TCCGGA T^CCGGA
MscI 155 TGGCCA TGG^CCA
MunI 1964 CAATTG C^AATTG
NaeI 815 GCCGGC GCC^GGC
NgoMIV 815 GCCGGC G^CCGGC
PflMI 635 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 858 AACGTT AA^CGTT
PvuII 49 CAGCTG CAG^CTG
SacI 954 GAGCTC GAGCT^C
SacII 257 CCGCGG CCGC^GG
SapI 2144 GCTCTTC GCTCTTC(1/4)
SmlI 732 CTTAAG C^T[CT][GA]AG
SphI 95 GCATGC GCATG^C
SspBI 1397 TGTACA T^GTACA
Van91I 635 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1637 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1821 GTCTAC GT^[AC][GT]AC
1946 GTCTAC
AccII 16 CGCG CG^CG
258 CGCG
AcsI 191 GAATTT [GA]^AATT[CT]
1747 GAATTT
AcyI 174 GACGCC G[GA]^CG[CT]C
2075 GACGCC
AlwNI 49 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
100 CAGCGGCTG
ApoI 191 GAATTT [GA]^AATT[CT]
1747 GAATTT
AspHI 825 GAGCAC G[AT]GC[AT]^C
954 GAGCTC
BanI 1538 GGTGCC G^G[CT][GA]CC
2095 GGTGCC
BbsI 40 GAAGAC GAAGAC(2/6)
203 GAAGAC
BpuAI 40 GAAGAC GAAGAC(2/6)
203 GAAGAC
BsaHI 174 GACGCC G[GA]^CG[CT]C
2075 GACGCC
BsaI 712 GAGACC GGTCTC(1/5)
2132 GAGACC
BsiHKAI 825 GAGCAC G[AT]GC[AT]^C
954 GAGCTC
BspLU11I 1394 ACATGT A^CATGT
1984 ACATGT
BstUI 16 CGCG CG^CG
258 CGCG
DraII 297 GGGTCCT [GA]G^G[ACGT]CC[CT]
1407 GGGTCCT
EcoO109I 297 GGGTCCT [GA]G^G[ACGT]CC[CT]
1407 GGGTCCT
HgaI 174 GACGC GACGC(5/10)
2075 GACGC
Hin1I 174 GACGCC G[GA]^CG[CT]C
2075 GACGCC
HindIII 1070 AAGCTT A^AGCTT
1756 AAGCTT
MvnI 16 CGCG CG^CG
258 CGCG
NcoI 20 CCATGG C^CATGG
1275 CCATGG
PpuMI 297 GGGTCCT [GA]G^G[AT]CC[CT]
1407 GGGTCCT
SspI 1331 AATATT AAT^ATT
1650 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII