Restriction map for
pFN21AE3120
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbsI BbvCI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 385 ACACGT A^C[GA][CT]GT
ApaI 431 GGGCCC GGGCC^C
Asp700I 139 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 872 CCCGGG C^[CT]CG[GA]G
AvaII 648 GGTCC G^G[AT]CC
BanII 431 GGGCCC G[GA]GC[CT]^C
BbrPI 734 CACGTG CAC^GTG
BciVI 878 GTATCC GTATCC(6/5)
BfaI 101 CTAG C^TAG
BsaAI 734 CACGTG [CT]AC^GT[GA]
BsmAI 97 GAGAC GTCTC(1/5)
BsmFI 389 GTCCC GGGAC(10/14)
BsmI 171 GCATTC GAATGC(1/-1)
BsoBI 872 CCCGGG C^[CT]CG[GA]G
BspMI 845 ACCTGC ACCTGC(4/8)
BstEII 861 GGTCACC G^GT[ACGT]ACC
BstPI 861 GGTCACC G^GT[ACGT]ACC
BstXI 629 CCATGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 22 GCGC GCG^C
EcoNI 846 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 861 GGTCACC G^GT[ACGT]ACC
EcoT14I 417 CCTTGG C^C[AT][AT]GG
HhaI 22 GCGC GCG^C
HinP1I 22 GCGC G^CGC
MaeI 101 CTAG C^TAG
MseI 475 TTAA T^TAA
MspA1I 766 CAGCTG C[AC]G^C[GT]G
NspI 690 GCATGT [GA]CATG^[CT]
PflMI 161 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 734 CACGTG CAC^GTG
PmlI 734 CACGTG CAC^GTG
PstI 652 CTGCAG CTGCA^G
PvuII 766 CAGCTG CAG^CTG
SexAI 316 ACCAGGT A^CC[AT]GGT
SfaNI 301 GCATC GCATC(5/9)
SfcI 652 CTGCAG C^T[GA][CT]AG
SmaI 872 CCCGGG CCC^GGG
SspI 214 AATATT AAT^ATT
StyI 417 CCTTGG C^C[AT][AT]GG
TaqI 711 TCGA T^CGA
Tru9I 475 TTAA T^TAA
Tsp45I 862 GTCAC ^GT[GC]AC
TthHB8I 711 TCGA T^CGA
Van91I 161 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 872 CCCGGG C^CCGGG
XmaI 872 CCCGGG C^CCGGG
XmnI 139 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 182 GTAC GT^AC
357 GTAC
AlwI 868 GATCC GGATC(4/5)
74 GGATC
BcnI 872 CCCGG CC^[GC]GG
873 CCGGG
BglII 62 AGATCT A^GATCT
563 AGATCT
BmyI 431 GGGCCC G[AGT]GC[ACT]^C
731 GGGCAC
BpmI 556 CTCCAG CTGGAG(16/14)
118 CTGGAG
Bsp1286I 431 GGGCCC G[AGT]GC[ACT]^C
731 GGGCAC
BsrDI 272 GCAATG GCAATG(2/0)
291 GCAATG
BstYI 62 AGATCT [GA]^GATC[CT]
563 AGATCT
Cac8I 821 GCTGGC GC[ACGT]^[ACGT]GC
825 GCTTGC
DraII 430 GGGGCCC [GA]G^G[ACGT]CC[CT]
431 GGGCCCT
EcoO109I 430 GGGGCCC [GA]G^G[ACGT]CC[CT]
431 GGGCCCT
HaeIII 432 GGCC GG^CC
856 GGCC
HapII 405 CCGG C^CGG
873 CCGG
HinfI 139 GAATC G^A[ACGT]TC
514 GAATC
HpaII 405 CCGG C^CGG
873 CCGG
MaeIII 704 GTTAC ^GT[ACGT]AC
862 GTCAC
MboII 561 GAAGA GAAGA(8/7)
840 GAAGA
MflI 62 AGATCT [GA]^GATC[CT]
563 AGATCT
MspI 405 CCGG C^CGG
873 CCGG
NciI 872 CCCGG CC^[GC]GG
873 CCGGG
NlaIV 430 GGGGCC GG[ACGT]^[ACGT]CC
431 GGGCCC
RsaI 182 GTAC GT^AC
357 GTAC
TfiI 139 GAATC G^A[AT]TC
514 GAATC
XhoII 62 AGATCT [GA]^GATC[CT]
563 AGATCT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HphI ItaI MaeII MboI MnlI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI TseI Tsp509I TspRI