Restriction map for
pFN21AE5623
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BciVI BfrI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfoI SfuI SgrAI
SmlI SpeI SphI SspBI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Aor51HI 128 AGCGCT AGC^GCT
AvaI 42 CCCGGG C^[CT]CG[GA]G
BalI 261 TGGCCA TGG^CCA
BbvCI 120 GCTGAGG CCTCAGC(-5/-2)
BcgI 200 GCATTGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 92 TGATCA T^GATCA
BfaI 650 CTAG C^TAG
BglI 235 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 470 CTGGAG CTGGAG(16/14)
BsaAI 493 TACGTA [CT]AC^GT[GA]
BseRI 250 GAGGAG GAGGAG(10/8)
BsgI 1016 CTGCAC GTGCAG(16/14)
BsiWI 495 CGTACG C^GTACG
BsmAI 1359 GAGAC GTCTC(1/5)
BsoBI 42 CCCGGG C^[CT]CG[GA]G
BsrBI 24 GAGCGG CCGCTC(-3/-3)
BstAPI 1018 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1240 GGTAACC G^GT[ACGT]ACC
BstPI 1240 GGTAACC G^GT[ACGT]ACC
CfoI 129 GCGC GCG^C
DsaI 245 CCATGG C^C[GA][CT]GG
EaeI 261 TGGCCA [CT]^GGCC[GA]
Eco47III 128 AGCGCT AGC^GCT
EcoO65I 1240 GGTAACC G^GT[ACGT]ACC
EcoRV 433 GATATC GAT^ATC
EcoT22I 256 ATGCAT ATGCA^T
FbaI 92 TGATCA T^GATCA
HaeII 128 AGCGCT [GA]GCGC^[CT]
HapII 43 CCGG C^CGG
HhaI 129 GCGC GCG^C
HinP1I 129 GCGC G^CGC
HindIII 632 AAGCTT A^AGCTT
HpaII 43 CCGG C^CGG
MaeI 650 CTAG C^TAG
MluNI 261 TGGCCA TGG^CCA
MscI 261 TGGCCA TGG^CCA
MspI 43 CCGG C^CGG
NcoI 245 CCATGG C^CATGG
NsiI 256 ATGCAT ATGCA^T
PstI 237 CTGCAG CTGCA^G
SbfI 236 CCTGCAGG CCTGCA^GG
SfcI 237 CTGCAG C^T[GA][CT]AG
SfiI 234 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 42 CCCGGG CCC^GGG
SnaBI 493 TACGTA TAC^GTA
Sse8387I 236 CCTGCAGG CCTGCA^GG
SspI 815 AATATT AAT^ATT
StuI 233 AGGCCT AGG^CCT
XmaCI 42 CCCGGG C^CCGGG
XmaI 42 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 415 AAATTT [GA]^AATT[CT]
685 GAATTT
AlwI 835 GATCC GGATC(4/5)
1087 GGATC
ApoI 415 AAATTT [GA]^AATT[CT]
685 GAATTT
BcnI 42 CCCGG CC^[GC]GG
43 CCGGG
BglII 402 AGATCT A^GATCT
981 AGATCT
BsiYI 42 CCCGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
714 CCGAGGCCAGG
BslI 42 CCCGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
714 CCGAGGCCAGG
BsrI 469 ACTGG ACTGG(1/-1)
264 CCAGT
BstF5I 150 CATCC GGATG(2/0)
1195 CATCC
Cfr13I 242 GGGCC G^G[ACGT]CC
274 GGCCC
FokI 150 CATCC GGATG(9/13)
1195 CATCC
HphI 1356 GGTGA GGTGA(8/7)
333 TCACC
MaeII 494 ACGT A^CGT
1211 ACGT
MslI 159 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
334 CACCGAGATG
NciI 42 CCCGG CC^[GC]GG
43 CCGGG
NlaIV 274 GGCCCC GG[ACGT]^[ACGT]CC
935 GGAACC
PleI 112 GAGTC GAGTC(4/5)
190 GAGTC
Sau96I 242 GGGCC G^G[ACGT]CC
274 GGCCC
Tsp45I 332 GTCAC ^GT[GC]AC
466 GTGAC
TspRI 265 CAGTG CA[GC]TG[ACGT][ACGT]^
768 CACTG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsmFI BsrDI BssKI BstNI BstYI
Cac8I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI HaeIII HinfI ItaI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XhoII