Restriction map for
pFN21AB4854
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AclI AflIII AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
BbrPI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DraI Eco47III EcoRV
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NruI NspI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1507 GACGTC GACGT^C
AccI 3432 GTCGAC GT^[AC][GT]AC
AccIII 1294 TCCGGA T^CCGGA
AflII 1108 CTTAAG C^TTAAG
Alw44I 775 GTGCAC G^TGCAC
AocI 2749 CCTAAGG CC^T[ACGT]AGG
ApaI 2159 GGGCCC GGGCC^C
ApaLI 775 GTGCAC G^TGCAC
Asp700I 2815 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2155 CCTAGG C^CTAGG
BbeI 1795 GGCGCC GGCGC^C
BbvCI 1781 CCTCAGC CCTCAGC(-5/-2)
BcgI 3019 GCAGCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1015 GTATCC GTATCC(6/5)
BclI 2112 TGATCA T^GATCA
BfrI 1108 CTTAAG C^TTAAG
BglII 880 AGATCT A^GATCT
BlnI 2155 CCTAGG C^CTAGG
BseAI 1294 TCCGGA T^CCGGA
BspDI 322 ATCGAT AT^CGAT
BspEI 1294 TCCGGA T^CCGGA
BstAPI 3648 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3594 GGTCACC G^GT[ACGT]ACC
BstPI 3594 GGTCACC G^GT[ACGT]ACC
Bsu36I 2749 CCTAAGG CC^T[ACGT]AGG
ClaI 322 ATCGAT AT^CGAT
DraIII 47 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2749 CCTAAGG CC^T[ACGT]AGG
EcoO65I 3594 GGTCACC G^GT[ACGT]ACC
EcoRI 3559 GAATTC G^AATTC
FbaI 2112 TGATCA T^GATCA
HgaI 1834 GACGC GACGC(5/10)
HincII 3432 GTCGAC GT[CT]^[GA]AC
HindII 3432 GTCGAC GT[CT]^[GA]AC
KasI 1795 GGCGCC G^GCGCC
MroI 1294 TCCGGA T^CCGGA
MslI 3311 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1795 GGCGCC GG^CGCC
NotI 1825 GCGGCCGC GC^GGCCGC
PaeR7I 40 CTCGAG C^TCGAG
PflFI 2001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3435 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3109 GAGCTC GAGCT^C
SalI 3432 GTCGAC G^TCGAC
SapI 2522 GCTCTTC GCTCTTC(1/4)
SexAI 3620 ACCTGGT A^CC[AT]GGT
SfoI 1795 GGCGCC GGC^GCC
Tth111I 2001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 40 CTCGAG C^TCGAG
XmnI 2815 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 982 AAATTT [GA]^AATT[CT]
3559 GAATTC
AcyI 1507 GACGTC G[GA]^CG[CT]C
1795 GGCGCC
AhdI 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2038 GACCTGGAGTC
ApoI 982 AAATTT [GA]^AATT[CT]
3559 GAATTC
AspEI 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2038 GACCTGGAGTC
BalI 2534 TGGCCA TGG^CCA
3542 TGGCCA
BamHI 203 GGATCC G^GATCC
2330 GGATCC
BlpI 1113 GCTAAGC GC^T[ACGT]AGC
1962 GCTAAGC
Bpu1102I 1113 GCTAAGC GC^T[ACGT]AGC
1962 GCTAAGC
BsaHI 1507 GACGTC G[GA]^CG[CT]C
1795 GGCGCC
BsaWI 200 ACCGGA [AT]^CCGG[AT]
1294 TCCGGA
CelII 1113 GCTAAGC GC^T[ACGT]AGC
1962 GCTAAGC
DrdI 2001 GACAGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2316 GACCCTCCTGTC
Eam1105I 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2038 GACCTGGAGTC
EcoT22I 74 ATGCAT ATGCA^T
2654 ATGCAT
Hin1I 1507 GACGTC G[GA]^CG[CT]C
1795 GGCGCC
MluNI 2534 TGGCCA TGG^CCA
3542 TGGCCA
MscI 2534 TGGCCA TGG^CCA
3542 TGGCCA
NcoI 602 CCATGG C^CATGG
3065 CCATGG
NsiI 74 ATGCAT ATGCA^T
2654 ATGCAT
PflMI 1066 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3462 CCACCAGATGG
ScaI 1484 AGTACT AGT^ACT
3629 AGTACT
SmlI 40 CTCGAG C^T[CT][GA]AG
1108 CTTAAG
TfiI 343 GATTC G^A[AT]TC
3070 GAATC
Van91I 1066 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3462 CCACCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII