Restriction map for
pFN21AE3689
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BciVI BfrI BglI
BglII BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI Hin1I KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Aor51HI 1472 AGCGCT AGC^GCT
AseI 1098 ATTAAT AT^TAAT
AsnI 1098 ATTAAT AT^TAAT
BalI 53 TGGCCA TGG^CCA
BamHI 114 GGATCC G^GATCC
BanII 244 GAGCTC G[GA]GC[CT]^C
BbvCI 1717 GCTGAGG CCTCAGC(-5/-2)
BclI 690 TGATCA T^GATCA
BpmI 1691 CTCCAG CTGGAG(16/14)
BsaAI 446 TACGTA [CT]AC^GT[GA]
BsaI 1592 GGTCTC GGTCTC(1/5)
BsaWI 117 TCCGGT [AT]^CCGG[AT]
BseRI 25 CTCCTC GAGGAG(10/8)
BsmAI 1593 GTCTC GTCTC(1/5)
BsrFI 1985 GCCGGT [GA]^CCGG[CT]
BstXI 101 CCACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 114 GGATCC [GA]^GATC[CT]
Cac8I 1833 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 1985 GCCGGT [GA]^CCGG[CT]
DsaI 932 CCATGG C^C[GA][CT]GG
EaeI 53 TGGCCA [CT]^GGCC[GA]
Eco47III 1472 AGCGCT AGC^GCT
FbaI 690 TGATCA T^GATCA
HgaI 581 GACGC GACGC(5/10)
HincII 1824 GTTAAC GT[CT]^[GA]AC
HindII 1824 GTTAAC GT[CT]^[GA]AC
HindIII 10 AAGCTT A^AGCTT
HpaI 1824 GTTAAC GTT^AAC
MaeII 447 ACGT A^CGT
MflI 114 GGATCC [GA]^GATC[CT]
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
MspA1I 106 CAGCTG C[AC]G^C[GT]G
NcoI 932 CCATGG C^CATGG
PshAI 379 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1098 ATTAAT AT^TAAT
PvuII 106 CAGCTG CAG^CTG
SacI 244 GAGCTC GAGCT^C
ScaI 541 AGTACT AGT^ACT
SfcI 1430 CTGTAG C^T[GA][CT]AG
SnaBI 446 TACGTA TAC^GTA
SspI 1742 AATATT AAT^ATT
SwaI 1548 ATTTAAAT ATTT^AAAT
XcmI 101 CCACCCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 114 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 244 GAGCTC G[AT]GC[AT]^C
598 GAGCAC
AvaI 187 CCCGAG C^[CT]CG[GA]G
961 CCCGAG
AvaII 39 GGTCC G^G[AT]CC
504 GGTCC
BcgI 1966 CGACATTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
274 GCATCCGAATCG
BcnI 162 CCGGG CC^[GC]GG
204 CCGGG
BlpI 126 GCTGAGC GC^T[ACGT]AGC
1475 GCTTAGC
BmyI 244 GAGCTC G[AGT]GC[ACT]^C
598 GAGCAC
Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC
1475 GCTTAGC
BsgI 752 CTGCAC GTGCAG(16/14)
1846 CTGCAC
BsiHKAI 244 GAGCTC G[AT]GC[AT]^C
598 GAGCAC
BsoBI 187 CCCGAG C^[CT]CG[GA]G
961 CCCGAG
Bsp1286I 244 GAGCTC G[AGT]GC[ACT]^C
598 GAGCAC
CelII 126 GCTGAGC GC^T[ACGT]AGC
1475 GCTTAGC
DraI 607 TTTAAA TTT^AAA
1549 TTTAAA
HaeII 123 GGCGCT [GA]GCGC^[CT]
1472 AGCGCT
MaeIII 227 GTGAC ^GT[ACGT]AC
1706 GTCAC
MwoI 622 GCTCATGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1976 GCCCTGTTTGC
NciI 162 CCGGG CC^[GC]GG
204 CCGGG
NdeI 1781 CATATG CA^TATG
2015 CATATG
PflMI 71 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
105 CCAGCTGGTGG
PleI 1612 GACTC GAGTC(4/5)
1562 GAGTC
SmlI 259 CTCAAG C^T[CT][GA]AG
1595 CTCAAG
Tsp45I 227 GTGAC ^GT[GC]AC
1706 GTCAC
Van91I 71 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
105 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BpuAI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MboI MboII MnlI MseI
MslI MspI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI