Restriction map for
pFN21AE1890
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccIII AcsI AcyI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BciVI BfrI BsaBI BsaHI
BsaI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 238 GTCTAC GT^[AC][GT]AC
AclI 258 AACGTT AA^CGTT
AflIII 941 ACATGT A^C[GA][CT]GT
AlwI 110 GGATC GGATC(4/5)
AlwNI 1221 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 914 AGCGCT AGC^GCT
AspHI 517 GAGCAC G[AT]GC[AT]^C
AspI 232 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 644 TGCGCA TGC^GCA
AvrII 825 CCTAGG C^CTAGG
BbsI 358 GAAGAC GAAGAC(2/6)
BcgI 480 GCAACCTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 860 TGATCA T^GATCA
BglI 845 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 665 AGATCT A^GATCT
BlnI 825 CCTAGG C^CTAGG
BlpI 409 GCTGAGC GC^T[ACGT]AGC
BmyI 517 GAGCAC G[AGT]GC[ACT]^C
BpmI 75 CTCCAG CTGGAG(16/14)
Bpu1102I 409 GCTGAGC GC^T[ACGT]AGC
BpuAI 358 GAAGAC GAAGAC(2/6)
BsgI 1218 GTGCAG GTGCAG(16/14)
BsiEI 1247 CGGCCG CG[GA][CT]^CG
BsiHKAI 517 GAGCAC G[AT]GC[AT]^C
BsmAI 1076 GAGAC GTCTC(1/5)
BsmI 214 GAATGC GAATGC(1/-1)
Bsp1286I 517 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 941 ACATGT A^CATGT
BsrDI 152 GCAATG GCAATG(2/0)
BsrFI 692 ACCGGC [GA]^CCGG[CT]
BstAPI 1025 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 665 AGATCT [GA]^GATC[CT]
CelII 409 GCTGAGC GC^T[ACGT]AGC
Cfr10I 692 ACCGGC [GA]^CCGG[CT]
EaeI 1247 CGGCCG [CT]^GGCC[GA]
EagI 1247 CGGCCG C^GGCCG
EarI 471 GAAGAG CTCTTC(1/4)
EclXI 1247 CGGCCG C^GGCCG
Eco47III 914 AGCGCT AGC^GCT
Eco52I 1247 CGGCCG C^GGCCG
FbaI 860 TGATCA T^GATCA
FspI 644 TGCGCA TGC^GCA
HaeII 914 AGCGCT [GA]GCGC^[CT]
Ksp632I 471 GAAGAG CTCTTC(1/4)
KspI 846 CCGCGG CCGC^GG
MaeIII 1178 GTTAC ^GT[ACGT]AC
MflI 665 AGATCT [GA]^GATC[CT]
NcoI 177 CCATGG C^CATGG
NotI 1246 GCGGCCGC GC^GGCCGC
PflFI 232 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 258 AACGTT AA^CGTT
PstI 933 CTGCAG CTGCA^G
SacII 846 CCGCGG CCGC^GG
SphI 1043 GCATGC GCATG^C
Tsp509I 460 AATT ^AATT
Tth111I 232 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 665 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BfaI 826 CTAG C^TAG
1106 CTAG
BsaAI 751 TACGTG [CT]AC^GT[GA]
802 TACGTG
BsaWI 521 ACCGGA [AT]^CCGG[AT]
1127 TCCGGT
BsmFI 938 GGGAC GGGAC(10/14)
775 GTCCC
BspMI 1181 ACCTGC ACCTGC(4/8)
1220 GCAGGT
BsrI 201 ACTGG ACTGG(1/-1)
269 ACTGG
BssSI 952 CACGAG CACGAG(-5/-1)
1095 CTCGTG
DsaI 177 CCATGG C^C[GA][CT]GG
846 CCGCGG
EcoNI 329 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
825 CCTAGGCGAGG
MaeI 826 CTAG C^TAG
1106 CTAG
MspA1I 78 CAGCGG C[AC]G^C[GT]G
846 CCGCGG
PpuMI 773 AGGTCCC [GA]G^G[AT]CC[CT]
833 AGGACCC
SfcI 443 CTACAG C^T[GA][CT]AG
933 CTGCAG
SmaI 656 CCCGGG CCC^GGG
878 CCCGGG
XmaCI 656 CCCGGG C^CCGGG
878 CCCGGG
XmaI 656 CCCGGG C^CCGGG
878 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BsaJI BseRI
BsiYI BslI BsoBI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
TspRI TthHB8I