Restriction map for
pFN21AB0482
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BglII BlnI
BlpI Bpu1102I BsaAI BsgI BsiEI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
FspI HgaI KasI KpnI KspI
MflI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI SspBI SwaI XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1377 GACGTC GACGT^C
AccIII 562 TCCGGA T^CCGGA
AcyI 1377 GACGTC G[GA]^CG[CT]C
ApaI 620 GGGCCC GGGCC^C
AspI 257 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 698 TGGCCA TGG^CCA
BanI 871 GGTGCC G^G[CT][GA]CC
BglI 1501 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3025 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1377 GACGTC G[GA]^CG[CT]C
BsaI 1181 GAGACC GGTCTC(1/5)
BseAI 562 TCCGGA T^CCGGA
BsmI 2552 GCATTC GAATGC(1/-1)
BspEI 562 TCCGGA T^CCGGA
BstBI 1222 TTCGAA TT^CGAA
BstEII 1398 GGTGACC G^GT[ACGT]ACC
BstPI 1398 GGTGACC G^GT[ACGT]ACC
CfoI 911 GCGC GCG^C
DrdI 2394 GACCCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 698 TGGCCA [CT]^GGCC[GA]
EcoO65I 1398 GGTGACC G^GT[ACGT]ACC
FauI 2833 GCGGG CCCGC(4/6)
HaeII 910 AGCGCC [GA]GCGC^[CT]
HhaI 911 GCGC GCG^C
Hin1I 1377 GACGTC G[GA]^CG[CT]C
HinP1I 911 GCGC G^CGC
HincII 2979 GTTAAC GT[CT]^[GA]AC
HindII 2979 GTTAAC GT[CT]^[GA]AC
HpaI 2979 GTTAAC GTT^AAC
MamI 3025 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1809 CAATTG C^AATTG
MluNI 698 TGGCCA TGG^CCA
MroI 562 TCCGGA T^CCGGA
MscI 698 TGGCCA TGG^CCA
MspA1I 451 CAGCTG C[AC]G^C[GT]G
MunI 1809 CAATTG C^AATTG
NruI 102 TCGCGA TCG^CGA
NspV 1222 TTCGAA TT^CGAA
PflFI 257 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 548 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2714 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 451 CAGCTG CAG^CTG
SbfI 471 CCTGCAGG CCTGCA^GG
ScaI 2917 AGTACT AGT^ACT
SfuI 1222 TTCGAA TT^CGAA
SpeI 3060 ACTAGT A^CTAGT
Sse8387I 471 CCTGCAGG CCTGCA^GG
SspI 2046 AATATT AAT^ATT
Tth111I 257 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 548 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 103 CGCG CG^CG
442 CGCG
AflII 217 CTTAAG C^TTAAG
2890 CTTAAG
AlwI 2938 GATCC GGATC(4/5)
158 GGATC
AlwNI 404 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
2124 CAGCACCTG
AocI 849 CCTGAGG CC^T[ACGT]AGG
2067 CCTCAGG
BbvCI 2121 CCTCAGC CCTCAGC(-5/-2)
883 GCTGAGG
BclI 651 TGATCA T^GATCA
938 TGATCA
BcnI 1998 CCGGG CC^[GC]GG
2254 CCGGG
BfrI 217 CTTAAG C^TTAAG
2890 CTTAAG
BsaWI 562 TCCGGA [AT]^CCGG[AT]
1472 TCCGGT
BsmBI 610 CGTCTC CGTCTC(1/5)
1379 CGTCTC
BsmFI 2165 GTCCC GGGAC(10/14)
2716 GTCCC
BstUI 103 CGCG CG^CG
442 CGCG
Bsu36I 849 CCTGAGG CC^T[ACGT]AGG
2067 CCTCAGG
DraI 312 TTTAAA TTT^AAA
3055 TTTAAA
DraIII 439 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
840 CACTGAGTG
Eco81I 849 CCTGAGG CC^T[ACGT]AGG
2067 CCTCAGG
EcoNI 468 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
789 CCTGATAAAGG
EcoRI 754 GAATTC G^AATTC
1972 GAATTC
EcoRV 2026 GATATC GAT^ATC
2033 GATATC
EcoT14I 357 CCTTGG C^C[AT][AT]GG
1403 CCAAGG
FbaI 651 TGATCA T^GATCA
938 TGATCA
MvnI 103 CGCG CG^CG
442 CGCG
NciI 1998 CCGGG CC^[GC]GG
2254 CCGGG
SapI 478 GCTCTTC GCTCTTC(1/4)
2599 GCTCTTC
StuI 1576 AGGCCT AGG^CCT
2355 AGGCCT
StyI 357 CCTTGG C^C[AT][AT]GG
1403 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI